Fig. 3 in Functional characterization of an Indian sandalwood (Santalum album L.) dual-localized bifunctional nerolidol/linalool synthase gene involved in stress response

Fig. 3. Subcellular localization of SaNES/LIS in Arabidopsis mesophyll protoplasts. The full-length coding region of SaNES/LIS was fused to the YFP reporter gene in the vector pSAT6-EYFP-N1 to produce the pSAT6- SaNES/LIS construct. The fusion construct were then transferred into Arabidopsis protoplasts and analyzed by confocal laser scanning microscopy for YFP expression. The yellow columns show YFP fluorescence, the blue column shows chlorophyll auto-fluorescence, the merged column shows combined YFP fluorescence and chlorophyll auto-fluorescence, and the transmission column shows light-microscopy images of the intact protoplasts. (A) Control pSAT6-YFP construct. (B, C) pSAT6-SaNES/LIS (expressed in plastids and the cytosol, respectively). Arrows 1 and 2 indicate granular fluorescence and diffuse fluorescence, respectively in the plastid. Fluorescence was excited for YFP at 514 nm and for Chl at 543 nm. Scale bar = 10 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)