Fig. 3 in Bioengineering of an elastase inhibitor from Caesalpinia echinata (Brazil wood) seeds

Fig. 3. Determination of rCeEI-based peptides inhibitory constants (Ki) for HNE. The enzyme was pre-incubated with rCeEI-36 (A) or rCeEI-46 (B) in different concentrations. The residual activity of the enzymes was determined using the synthetic substrate MeO-Suc-Ala-Ala-Pro-Val-pNan. The release of the ρ-nitroaniline was followed at 405 nm. Ki values were determined by adjusting the experimental data to the equation for slow tight binding using a non-linear regression.