Fig. 5 in A previously undescribed phenylethanoid glycoside from Callicarpa kwangtungensis Chun acts as an agonist of the Na/K-ATPase signal transduction pathway

Fig. 5. Effect of callicarpanoside B on NKA-mediated kinase activation in LLC-PK1 cells. A, LLCPK1 cells were treated with 10, 100, and 250 nM callicarpanoside B for 10 min, and the cell lysate was collected for Weston blot analysis showed in the upper panel. The data are combined from 3 to 8 separate experiments and are presented as mean ± SEM, shown in the lower panel. B, LLCPK1 cells were treated with 100 nM callicarpanoside B for 2, 10, 30, and 120 min, and the cell lysate was collected for Weston blot analysis, showed in the upper panel. The data are combined from 3 to 8 separate experiments and are presented as mean ± SEM. C, LLCPK1 cells were treated with 10, 100, and 250 nM callicarpanoside B for 10 min, and the cell lysate was collected for Weston blot. Probed for p-Src and showed in the upper panel. The data are combined from indicated separate experiments and are presented as mean ± SEM, shown in the lower panel. D, LLCPK1 cells were treated with 10, 100 nM callicarpanoside B for 15 min, and the lysates were fractioned into Cytosolic (Cy) and particulate (Pa) fractions for Weston blot analysis and Pa/Cy ratios of PKCε, contents were compared. The upper panel represents Western blot. The data are combined and presented as mean ± SEM of 3 independent experiments, shown in the lower panel. Student t-test, *, P <0.05; **, P <0.01.