Fig. 3 in CYP71BL9, the missing link in costunolide synthesis of sunflower

Fig. 3. Two alternative pathways of eupatolide biosynthesis. Comparison of the extracts of transiently transformed N. benthamiana leaves carrying the vector combinations [p19/DXS + HaGAS1 + HaGAO + HaG8H], [p19/DXS + HaGAS1 + HaGAO + HaG8H + HaES] and [p19/DXS + HaGAS1 + HaGAO + HaG8H + CYP71BL9]; A, LC-MS chromatograms; B, UV and (+) MS spectrum of peak d; C, UV and (+) MS spectrum of peak h; D, EIC (C18H27NO5S) [M +H]+ = 370; E, EIC (C H N O S) [M+H]+ = 556; F, scheme, conversion of germacrene A acid to eupatolide by HaG8H and HaES; G, scheme, conversion of 25 37 3 9 germacrene A acid to eupatolide by HaG8H and CYP71BL9