Fig. 5 in Biosynthesis and tissue-specific partitioning of camphor and eugenol in Ocimum kilimandscharicum

Fig. 5. Integration of transcriptomics and metabolomics data for understanding eugenol biosynthesis in O. kilimandscharicum (Ok). (A) Pathway genes and intermediate metabolites in eugenol biosynthesis in O. kilimandscharicum. Transcripts for the genes marked in red were present in the Ok transcriptome. Metabolites marked in black were putatively identified; however, those marked in blue could not be detected on the LC-Orbitrap [phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H), p-coumarate 3-hydroxylase (C3H), caffeic acid O-methyltransferse (COMT), 4-coumarate:CoA ligase (4CL), cinnamoyl-CoA reductase (CCR), cinnamyl alcohol dehydrogenase (CAD), alcohol acyltransferase (AAT), eugenol synthase (EGS)]. (B) Heat map analysis showing putatively identified intermediates detected on the LC-Orbitrap across different tissues [anther + pistil (A + P)]. All values represent an average of three biological replicates. The color scale for foldchange values is shown. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)