Fig. 4 in CYP71BL9, the missing link in costunolide synthesis of sunflower

Fig. 4. The expression of candidate gene CYP71BL9 in combination with HaG8H in yeast. A, HPLC comparison of extracts from yeast strains [EPY300:Leu2d:GAS/ CR/GAO/HaG8H||Ura:CYP71Bl9] and control [EPY300:Leu2d:GAS/CR/GAO/HaG8H||Ura:empty] peak a: CYP71BL9 enzyme product, peak b: enzyme product of HaG8H and CYP71BL9 at 15.61 min; B, DAD-chromatogram, peak b purified, in comparison with costunolide standard; C UV–Vis spectrum of peak b and costunolide; D, (+) MS2 spectrum of peak b; E, (+) MS2 spectrum of costunolide; F, reaction scheme for the generation of costunolide with HaG8H and CYP71BL9 (not shown: 8βhydroxy-germacrene A acid, 11.50 min)