Fig. 2 in CYP71BL9, the missing link in costunolide synthesis of sunflower

Fig. 2. Comparison of the expression of LsCOS and CYP71BL9 in planta. Comparison of extracts from N. benthamiana leaves transiently transformed with the vectors [p19/DXS + HaGAS1 + HaGAO], [p19/DXS + HaGAS1 + HaGAO+ LsCOS] und [p19/DXS + HaGAS1 + HaGAO + CYP71BL9]; A, LC-MS chromatograms; B, UV and (+) MS spectrum von peak a; C, Conversion of germacrene A acid to costunolide by LsCOS and CYP71BL9 as well as addition reaction to costunolide-glutathione (peak b); D, UV and (+) MS spectrum of peak b; peak a: costunolide-cysteine, Peak b: costunolide-glutathione, (*) costunolide-glutathione shown in EIC, (**) Traces of free costunolide shown (peak c); BPC: base peak count, EIC: extracted ion chromatogram