Fig. 2 in Triterpene glycosides from Blighia welwitschii and evaluation of their antibody recognition capacity in multiple sclerosis

Fig. 2. Data distribution of IgM antibody responses to saponins 2, 6, 7, hederagenin monodesmoside and giganteaside J from B. welwitschii, in MS patients' sera, determined by ELISA. CSF114(Glc) and its unglucosylated analogue, CSF114, were tested as positive and negative controls, respectively. Glc oleanolate from O. obtusifolia, whose glycoside part is significantly different from B. welwitschii saponins, was tested as a reference compound. Data are reported as absorbance at 405 nm of sera diluted 1:100. Horizontal lines represent mean values with their SD. Mann-Witney U test showed that the CSF114(Glc) distribution significantly differs from this of CSF114 (p value <0.0001, two-tailed). Kruskal-Wallis test including multiple comparison of natural glycosides distributions showed statistical differences between compound 2 and 6 (p value <0.05), 2 and 7 (p value <0.0001), 2 and hederagenin monodesmoside (p value <0.01), Glc oleanolate and hederagenin monodesmoside (p value <0.05), Glc oleanolate and 7 (p value <0.0001) and Glc oleanolate and 6 (p value <0.05).