Fig. 6 in Terpenoids from the Chinese liverwort Heteroscyphus coalitus and their antivirulence activity against Candida albicans

Fig. 6. The inhibitory effect of the compound 13 on C. albicans morphological transition. A. C. albicans DSY654 were grown in RPMI 1640 medium with a series of concentrations of 13. After 6 h incubation at 37 ̊C, the images were obtained using a microscope (scale bars: 25 μm). B. The A549 cells were co-incubated with C. albicans DSY654-TDH3-GFP cells for 90 min. Finally, cells were observed using a fluorescence microscope (scale bars: 50 μm). C. The number of adherent cells was determined by counting the cells in the pictures. Bars represent means ± SD. *P <0.05, **P <0.01, **P <0.001. D. After incubation of 24 h, the biofilm was washed three times with PBS and photographed by a microscope (scale bars: 50 μm). E. The biofilm was detected with an XTT reduction assay. Bars represent means ± SD. *P <0.05, **P <0.01, **P <0.001. F. C. albicans DSY654 was treated with 4 μg/ml of 13 for 6 h at 37 ̊C. The transcriptional levels of genes related to hyphal formation and adhesion were determined by qPCR and showed as a fold change relative to the negative group. Bars represent means ± SD. *P <0.05, **P <0.01, **P <0.001.