Fig. 5 in Alterations in nepetalactone metabolism during polyethylene glycol (PEG)- induced dehydration stress in two Nepeta species

Fig. 5. The expression of selected transcription factors (COI1, JAZ3, MYC2, MYB and YABBY5) during PEG-induced dehydration stress in Nepeta rtanjensis (a) and N. argolica ssp. argolica (b) leaves at 1, 3 and 6 days of PEG treatment (DPT). Obtained values were normalized against NrGAPDH (a) and NaGAPDH (b) as the endogenous controls and presented relatively to their respective controls as calibrators. Results are presented as the means ± SD of three biological replicates. Asterisks (*) denote significant differences between PEG-treated and non-treated plants (unpaired t-test, *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001). Abbreviations: COI1- coronatine-insensitive 1, JAZ3-jasmonate ZIM-domain 3, MYC2- transcription factor Mouse-ear cress 2, MYB- transcription factor containing MYB-domain, YABBY5- YABBY5 transcription factor, GADPH- glyceraldehyde-3-phosphate dehydrogenase. Heatmaps of Pearson correlations of the absolute expression values for nepetalactone biosynthetic genes and transcription factors in N. rtanjensis (c) and N. argolica ssp. argolica (d). Correlation coefficients are shown in the lower triangle, while statistical significance (p <0.01) is marked with an asterisk at the appropriate positions in the upper triangle. Each square indicates the Pearson's correlation coefficient for a pair of genes, and the value of the correlation coefficient is represented by the intensity of yellow (positive correlations) or grey (negative correlations) in N. rtanjensis, or green (positive) and grey (negative) in N. argolica ssp. argolica, as indicated on the colour scale (for the interpretation of the references to colour in this Figure legend, the reader is referred to the web version of this article). The correlogram is ordered based on hierarchical cluster analysis (HCA) performed on a distance matrix (1- correlation matrix: minimal distance for absolutely positively correlated and maximal distance for absolutely negatively correlated gene expression). Cluster agglomeration was done using the single-linkage clustering method. The dendrograms are shown on heatmap sides. Abbreviations: GPPS- geranyl diphosphate synthase, GES- geraniol synthase, G8O- geraniol 8-oxidase, 8HGO- 8-hydroxygeraniol oxidoreductase, IS- iridoid synthase, NEPS- nepetalactol-related short-chain dehydrogenase, COI1- coronatine-insensitive 1, JAZ3-jasmonate ZIM-domain 3, MYC2- transcription factor Mouse-ear cress 2, MYBtranscription factor containing MYB-domain, YABBY5- YABBY5 transcription factor, DPT-days of PEG treatment. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)