Fig. 4 in Pilot study on the uptake and modification of harmaline in acceptor plants: An innovative approach to visualize the interspecific transfer of natural products

Fig. 4. Confocal laser-scanning microscopy of a barley leaf incubated with harmaline. Five days after application of the alkaloid, leaves were examined by confocal laser-scanning microscopy (λex = 364 nm). The fluorescence spectra had been recorded for different areas or cell types, respectively, which are displayed by coloured circles; red: xylem; green and blue mesophyll. The analogous spectra are presented in the corresponding colour. According to the wavelength of maximal emission, the fluorescence could be attributed to lignin (xylem), harmaline (cells in the proximity of the vascular bundles) and harmine (cells more distant from them). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)