Fig. 6 in Alterations in nepetalactone metabolism during polyethylene glycol (PEG)- induced dehydration stress in two Nepeta species

Fig. 6. The relative expressions of the nepetalactone biosynthesis candidate genes (a) and of transcription factors (b) in trichomes and abraded leaves of Nepeta rtanjensis were normalized against 18S rRNA gene as endogenous control, and expressed relative to abraded leaves as calibrators. Error bars indicate SD of three biological replicates, each analysed in technical triplicates. Asterisks (*) denote significant differences between trichomes and depleted leaves (paired t-test, *p ≤ 0.05; **p ≤ 0.001; ***p ≤ 0.0001). Light micrograph of detached leaf trichomes (c) showing individual peltate glandular trichomes (PGT) and non-glandular mechanical trichomes (MT), bar = 100 μm. The light microscope (Leica DM2000, Germany) was used for the analysis of detached trichomes from N. rtanjensis leaves and image capturing was acquired with Software Leica IM1000 (Leica, Germany). Abbreviations: GPPSgeranyl diphosphate synthase, GES- geraniol synthase, G8O- geraniol 8-oxidase, 8HGO- 8-hydroxygeraniol oxidoreductase, IS- iridoid synthase, NEPS- nepetalactol-related short-chain dehydrogenase, COI1- coronatine-insensitive 1, JAZ3- jasmonate ZIM-domain 3, MYC2- transcription factor Mouse-ear cress 2, MYB- transcription factor containing MYB-domain, YABBY5- YABBY5 transcription factor.