Fig. 3 in Alterations in nepetalactone metabolism during polyethylene glycol (PEG)- induced dehydration stress in two Nepeta species

Fig. 3. Proposed nepetalactone (NL) biosynthetic pathway in Nepeta rtanjensis (Nr) and N. argolica ssp. argolica (Na) constructed based on the partially characterized N. mussini pathway (Lichman et al., 2018) and known MIA pathway in Catharanthus roseus (Miettinen et al., 2014). Arrows represent characterized biosynthetic steps in N. mussinii (Nm) or C. roseus (Cr). Dashed arrows represent parts of the NL-biosynthetic pathway specific for N. rtanjensis and N. argolica ssp. argolica. Enzymes involved in N. rtanjensis NL-metabolism are marked bold yellow and the ones included in the pathway of N. argolica ssp. argolica are bold green. Question marks (?) indicate unknown enzymes. Extracted are UHPLC/SRM−MS2 chromatograms of cis,trans-NL, trans,cis-NL and dehydronepetalactone (DNL). Abbreviations: GPPgeranyl diphosphate, IPP- isopentenyl diphosphate, DMAPP- dimethylallyl diphosphate, GPPS- geranyl diphosphate synthase, GES- geraniol synthase, G8O- geraniol 8-oxidase, 8HGO- 8-hydroxygeraniol oxidoreductase, IS- iridoid synthase, NEPS- nepetalactol-related short-chain dehydrogenase. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)