Fig. 1 in Vitamin E in legume nodules: Occurrence and antioxidant function

Fig. 1. Identification and quantification of tocochromanols in legume nodules. (A) HPLC chromatograms (left) and LC-MS/MS identification (right) of α-tocopherol in nodules of soybean, alfalfa and pea plants. Precursor and product ion m/z used for LC-MS/MS identification was 430/163.1) α-tocopherol, 2) β-tocopherol, 3) unknown, 4) unknown. (B) Quantification of tocochromanols in leaves, roots and nodules of three studied legumes (soybean, alfalfa and pea plants). Data represent the mean ± SE of n = 10. Although peaks 3 and 4 found in pea nodules co-eluted with α- and γ-tocotrienol, respectively, in the HPLC chromatogram, they could not be confirmed by LC-MS/MS. BHT, butylated hydroxytoluene.