Fig. 5 in Function of ceramide synthases on growth, ganoderic acid biosynthesis and sphingolipid homeostasis in Ganoderma lucidum

Fig. 5. Sphingolipid profiling of the lag-silenced, WT and SiControl strains. The relative amounts of Cer (A), GlcCer (B), IPC (C), MIPC (D) and M (IP)2C (E) in each strain. Comparison of the sphingolipid intensity ratios between the WT and lag-silenced strains. Each sphingolipid (Cer/GlcCer/IPC/ MIPC/M(IP)2C) intensity ratio was calculated as the percentage of the corresponding total sphingolipid detected in the WT or mutant. The sphingolipid species are indicated as the number of carbon atoms: the unsaturated bond number of the fatty acid: hydroxyl number. For example, Cer 32:0:2 = Cer (d18:0/14:0); Cer 36:0:3 = Cer (t18:0/18:0 or d18:0/18:0 (2-OH)); Cer 36:0:4 = Cer(t18:0/18:0 (2-OH)); Cer 38:1:2 = Cer (d18:0/20:1 or d18:1/ 20:0) and Cer 42:2:2 = (d18:0/24:2, d18:2/24:0 or d18:1/24:1). There are three independent biological replicates in each column. Error bars represent standard deviations, and asterisks show significant differences from control (WT and Sicontrol) strains according to Student's t-test (*P <0.05, **P <0.01, n = 3).