Fig. 5. Compound 2 induced apoptosis through a mitochondria-dependent pathway. A in Bufadienolides from the whole plants of Helleborus foetidus and their cytotoxicity

Fig. 5. Compound 2 induced apoptosis through a mitochondria-dependent pathway. A, Mitochondrial membrane morphology of HL-60 cells treated with 2. HL-60 cells were stained with MitoCapture™ reagent after treatment with either 0.5 μM of 2 or 33 μM of cisplatin for 10 h and observed under a fluorescence microscope. B, Release of cytochrome c from mitochondria of HL-60 cells treated with 2. HL-60 cells were treated with either 0.5 μM of 2 or 33 μM of cisplatin for 6 h, and the release of cytochrome c into the cytoplasm was evaluated by Western blot analysis.