Fig. 13 in Glucosinolate structural diversity, identification, chemical synthesis and metabolism in plants

Fig. 13. The usefulness of the COSY spectrum for establishing connectivity, and an example of NMR of an intact glucosinolate (GSL). (A) The 1H NMR spectrum of 3-(hydroxymethyl)methylpentyl GSL (141) in D2O. A major impurity of acetate from the ion exchange isolation is seen. (B) The corresponding COSY spectrum. Signals along the diagonal correspond to the 1D spectrum. Whenever protons are found at the same or neighboring carbons, a "cross peak" can be seen with the x-coordinate of the one signal and the y-coordinate of the other. Original data by the authors (CEO and NA, Sections 6.1 and 6.2).