Fig. 7 in Identification, characterization, and antifungal activity of cysteine peptidases from Calotropis procera latex

Fig. 7. Detection of ROS in spores of F. oxysporum after treatment with latex peptidases (CpCP1, CpCP2 and CpCP3) or inhibited with iodoacetamide (CpCP 1-IAA, CpCP 2-IAA and CpCP 3-IAA). Uptake of DAB was identified by the presence of a reddish-brown precipite. Samples (50 μg/mL) were incubated with spores for 30 min at 27 ̊C, pH 7.0. Control: 50 mM sodium phosphate buffer (pH 7.0). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)