Fig. 3 in Identification, characterization, and antifungal activity of cysteine peptidases from Calotropis procera latex

Fig. 3. Effect of pH, temperature and substrate concentration on the proteolytic activity of CpCP1, CpCP2 and CpCP3. In (A) and (B), the assays were performed using 1 mM BApNA as substrate. In B, the proteolytic activity was assayed at pH 7.0. In (C), the assays were performed using different concentrations of BApNA at pH 7.0, 35 ̊C, and 100 μg of each peptidase. Km and Vmax were determined from a nonlinear curve fit of the untransformed data using GraphPad Prism 7. The numbers used to define the kinetic parameters were best-fit estimate ± SE.