Fig. 4 in Catalysis by allene oxide synthases (CYP74A and CYP74C): Alterations by the Phe/Leu mutation at the SRS-1 region

Fig. 4. GC-MS analyses of products (Me/TMS) of 9(S)-HPOT conversions by recombinant WT ZmAOS1 (А), PpAOS2 F93L (B), and ZmAOS1 F95L (C). 2a and 2b, threo and erythro isomers of 9,10-dihydroxy-12,15-octadecadienoic acid; 5, 9,10-epoxy-11-hydroxy-12,15-octadecadienoic acid; 11a and 11b, threo and erythro isomers of 9,10-epoxy-13-hydroxy-11,15-octadecadienoic acid; 13, 9-hydroxynonanoic acid. The structural formulae of products are presented in Fig. 2. 9-HOT, (9S,10E,12Z,15Z)-9-hydroxy-10,12,15-octadecatrienoic acid.