Raw diffraction images of [NiFe]-hydrogenase maturation factor HypD from Aquifex aeolicus (C360S mutant)

<p>HypD is one of the maturation factors of [NiFe]-hydrogenase and can form a complex with other maturation factors&nbsp;(Muraki&nbsp;<em>et al.</em>, 2019).&nbsp;</p> <p>We used datasets to investigate the protocol of detecting polymorphs using Hierarchical clustering (Acta D., submitted). All diffraction datasets were collected at BL45XU, SPring-8, using an automated data collection system&nbsp;<em>ZOO</em>. From six crystals, datasets were collected from each one using a continuous helical scan scheme for 360&ordm; oscillation with the following experimental parameters; Beam size: 20 &micro;m &times; 20 &micro;m, Wavelength: 1.0000 &Aring;, Total dose/crystal: 10 MGy, Detector: EIGER X 9M (DECTRIS Co. Ltd.). All crystals belonged to space group P212121 with unit cell parameters roughly corresponding to a=60.0, b=62.6, c=97.6 &Aring;.</p>