Fig. 4 in Analysis of Water-Soluble Proteins by Two-Dimensional Electrophoresis in the Encystment Process of Colpoda cucullus Nag-1 and Cytoskeletal Dynamics

Fig. 4. NIH image analyses of CBB-stained α-tubulin spots of gels (closed circles) separated by 2-D PAGE of water-insoluble fraction (So- game et al. 2014b), CBB-stained β-tubulin fragment spots of gels (p19 in Fig. 2, open circles) contained in water-soluble fraction, and total α-tubulin immunoblotting bands (open squares) (Fig. 3) of C. cucullus Nag-1. Ordinate indicates integrated optical density (IOD) reflecting relative protein amounts. Abscissa indicates the time lapse after onset of encystment induction.