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Genotyping of European Toxoplasma gondii strains by a new high-resolution next-generation sequencing-based method

<p>The data set comprises 164&nbsp;FASTQ&nbsp;files generated with an Ion AmpliSeq-based genotyping method for <em>Toxoplasma gondii </em>and<em>&nbsp;</em>a BED file used for the design of the Ion AmpliSeq primer panel. The FASTA&nbsp;file named as "AmpliSeq-ME49-Reference" was used as a reference for mapping and data analysis of the FASTQ files. The GZ&nbsp;file named as "Tgondii_IonAmpliSeq_Results_SNPs_VCF" is a VCF file, which contains&nbsp;all SNPs identified within the 164 FASTQ files relative to the AmpliSeq-ME49-Reference. The VCF&nbsp;file was converted into a FASTA file named as "Tgondii_IonAmpliSeq_Results_SNPs", which also contains the&nbsp;SNPs identified within the 164 FASTQ files relative to the AmpliSeq-ME49-Reference.</p> <p>The work is published in the European Journal of Clinical Microbiology &amp; Infectious Diseases with the title "Genotyping of European <em>Toxoplasma gondii</em> strains by a new high‑resolution next‑generation sequencing‑based method";&nbsp;https://doi.org/10.1007/s10096-023-04721-7</p>

ShareScore

44/100

Overall dataset sharing score

Score breakdown

These five areas show where the dataset supports — or may limit — practical reuse.

Stewardship
12
Harmonization
4
Access
12
Reuse readiness
8
Engagement
8

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