RNA-seq based analysis of gene expression in thyroids of wild-type and Keap1 knockdown mice after exposure to excess iodide.

<p>C57BL/6J Keap1flox/flox mice were developed by Prof. Masayuki Yamamoto (DOI: 10.1016/j.bbrc.2005.10.185). These mice express lower levels of Keap1 because of the loxP site insertions (DOI: 10.1128/MCB.01591-09)&nbsp;and are designated as Keap1KD. 3-4 months old WT and Keap1KD mice fed a standard diet&nbsp; (KLIBA NAFAG 3242, Switzerland) containing 1.5 mg/kg sodium iodine were given normal tap water with or without 0.05% sodium iodide&nbsp; (Sigma, St Louis, MO, USA) for 7 days. Hence, the following groups of mice were included:</p> <p>WT mice on control water (n=8)-designated as wt in the file</p> <p>WT mice exposed to excess iodide (WT-IOD, n=6)- designated as wti in the file</p> <p>Keap1KD mice on control water (n=8)- designated as kp&nbsp;in the file</p> <p>&nbsp;and Keap1KD mice exposed to excess iodide (Keap1KD-IOD, n=6)- designated as kpI in the file.&nbsp;</p> <p>&nbsp;Mice were maintained in the animal facility of the Department of Physiology at&nbsp; the&nbsp; University&nbsp; of&nbsp; Lausanne&nbsp; in temperature-,&nbsp; light-,&nbsp; and&nbsp; humidity-controlled&nbsp; rooms&nbsp; with&nbsp; a 12-hour light/dark cycle. All animal procedures were in accordance with Swiss legislature and the study was approved by the Canton of Vaud SCAV.</p> <p>RNA from individual mouse thyroids was prepared as previously described (DOI: 10.1007/978-1-4939-3756-1_25)&nbsp;and was submitted to Alithea Genomics (Switzerland). The bulk RNA barcoding and sequencing (BRB-seq) libraries were generated and sequenced as described previously (DOI: 10.1186/s13059-019-1671-x) to a depth of approximately 1.2 million raw reads per sample.</p> <p>This research was funded by the Swiss National Science Foundation Research Grants 310030_212558,&nbsp; IZCOZ0_205415 and IZCOZ0_177070</p> <p></p> <p></p>