rCRUX Generated Kelly Metazoan 16S Reference Database

<p>rCRUX generated reference database&nbsp;using NCBI nt blast database downloaded in December 2022.</p> <p>Primer Name:&nbsp; Kelly Metazoan 16S<br> Gene:&nbsp; &nbsp;16S<br> Length of Target:&nbsp; &nbsp; 114-160<br> get_seeds_local() minimum length:&nbsp; &nbsp; 80<br> get_seeds_local() maximum length:&nbsp; &nbsp; 195<br> blast_seeds() minimum length:&nbsp; &nbsp; 50<br> blast_seeds() maximum length:&nbsp; &nbsp; 168<br> max_to_blast:&nbsp;&nbsp;100<br> Forward Sequence (5&#39;-3&#39;):&nbsp; &nbsp;AGTTACYYTAGGGATAACAGCG<br> Reverse Sequence (5&#39;-3&#39;):&nbsp; &nbsp; CCGGTCTGAACTCAGATCAYGT<br> Reference:&nbsp; &nbsp; Kelly, R. P., O&rsquo;Donnell, J. L., Lowell, N. C., Shelton, A. O., Samhouri, J. F., Hennessey, S. M., ... &amp; Williams, G. D. (2016). Genetic signatures of ecological diversity along an urbanization gradient. PeerJ, 4, e2444.&nbsp;https://doi.org/10.7717/peerj.2444</p> <p>We chose default rCRUX parameters for&nbsp;<em>get_blast_seeds</em>() of percent coverage of 70, percent identity of 70, evalue 3e+7, and max number of blast alignments = &#39;100000000&#39; and for&nbsp;<em>blast_seeds</em>() of coverage of 70, percent identity of 70, evalue 3e+7, rank of genus, and max number of blast alignments = &#39;10000000&#39;. &nbsp;</p>