rCRUX Generated MiSebastes Reference Database

<p>rCRUX generated reference database&nbsp;using NCBI nt blast database downloaded in December 2022.</p> <p>Primer Name:&nbsp; MiSebastes<br> Gene:&nbsp; &nbsp;CytB<br> Length of Target:&nbsp; &nbsp; 153<br> get_seeds_local() minimum length:&nbsp; &nbsp; 107<br> get_seeds_local() maximum length:&nbsp; &nbsp; 199<br> blast_seeds() minimum length:&nbsp; &nbsp; 71<br> blast_seeds() maximum length:&nbsp; &nbsp; 163<br> max_to_blast:&nbsp; 100<br> Forward Sequence (5&#39;-3&#39;):&nbsp; &nbsp;AAGCTCATTCAAGTGCTT<br> Reverse Sequence (5&#39;-3&#39;):&nbsp; &nbsp; GACCACTTACACAATTCT<br> Reference:&nbsp; &nbsp;Min, M. A., Barber, P. H., &amp; Gold, Z. (2021). MiSebastes: An eDNA metabarcoding primer set for rockfishes (genus Sebastes). Conservation Genetics Resources, 13(4), 447-456.&nbsp;https://doi.org/10.1007/s12686-021-01219-2</p> <p>We chose default rCRUX parameters for&nbsp;<em>get_blast_seeds</em>() of percent coverage of 70, percent identity of 70, evalue 3e+7, and max number of blast alignments = &#39;100000000&#39; and for&nbsp;<em>blast_seeds</em>() of coverage of 70, percent identity of 70, evalue 3e+7, rank of genus, and max number of blast alignments = &#39;10000000&#39;. &nbsp;</p>