An Intravenous Pancreatic Cancer Therapeutic: Characterization of CRISPR/Cas9n-modified Clostridium novyi-Non Toxic Supplementary Information Histology Slides Part 4

<p>H&amp;E and Gram stained whole slide scans for blinded cohorts from&nbsp;publication titled &lsquo;An Intravenous Pancreatic Cancer Therapeutic: Characterization of CRISPR/Cas9n-modified <em>Clostridium novyi-</em>Non Toxic&rsquo; published in PLOS One 2023.</p> <p>Excerpt from methods section of publication detailing acquisition of this data: &quot;Formalin-fixed tissue samples were processed for paraffin embedding by the means of dehydration, clearing and paraffin infiltration (Lynx II Tissue Processor). The paraffin embedded tissue samples were sectioned using a Leica Rotatory Microtome RM2125 RTS at 5um thickness. Designated tissue sections were&nbsp;subsequently de-paraffined and stained with H&amp;E and gram staining as per standard histology protocols (Leica Autostainer XL). Whole slide scanning (WSI) was performed by a Panoramic 250 whole slide scanner at 20x magnification (3D Histech) using a Carl-Zeiss Plan-Apochromat 20x / NA 0.8 objective.&quot;</p> <p>Please note when downloading the image files that the folder containing all of the .data files <strong>cannot</strong> contain the corresponding .mxrs file. The .mxrs and the folder containing the .data files <strong>must</strong> have identical names and be at the same file level (as uploaded). Files of this type can be opened by the open source software FIJI or QuPath, among others.</p> <p>Cohorts have been uploaded with viewers blinded to treatment groups to allow for unbiased review should it be desired, with a cohort key uploaded separately (doi: ##). Files are grouped so that all major organs from a single mouse are in the same compressed folder.</p>