Multi-colour SMLM images of untreated and drug-treated Escherichia coli (LB, exponential phase)

<p>This dataset and CARE model is part of the publication &quot;<strong>Transertion and cell geometry organize the <em>Escherichia coli</em> nucleoid during rapid growth</strong>&quot;.</p> <p>It contains all SMLM images that were used for the publication, as well as the single-cell regions of interest for analyses.</p> <p>Cells were grown to exponential phase in LB Lennox and antibiotics were added for 0-60 min. Cultures were then chemically fixed, permeabilised and imaged for the nucleoid (JF₆₄₆-Hoechst) and membranes (Nile Red) using PAINT. The strain (NO34) expresses a MreB^sw-sfGFP fusion protein from the native chromosomal locus. It was a kind gift from Zemer Gitai (<a href="http://doi:10.1016/j.bpj.2016.07.017">Ouzounov et al., 2016</a>).</p> <p>More information can be found in the publication.</p>