Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
1,952
datasets available to search
ShareScore release 0.9.0
Dataset results
1,952 results for “actionality”
Drp1 controls Complex II assembly and skeletal muscle metabolism by Sdhaf2 action on mitochondria
<p>The Dynamin-related GTPase, Drp1 (encoded by <em>Dnm1l</em>) plays a central role in mitochondrial fission and is requisite for numerous cellular processes however its role in muscle metabolism remains unclear. Herein, we show that among human tissues, the highest number of gene correlations with <em>DNM1L </em> are in skeletal muscle. Knockdown of Drp1 (Drp1-KD) promoted mitochondrial hyperfusion in the muscle of male mice. Reduced fatty acid oxidation and impaired insulin action along with increased muscle succinate was observed in Drp1-KD muscle. Muscle Drp1-KD reduced Complex II assembly and activity as a consequence of diminished mitochondrial translocation of succinate dehydrogenase assembly factor 2 (Sdhaf2). Restoration of Sdhaf2 normalized Complex II activity, lipid oxidation, and insulin action in Drp1-KD myocytes. Drp1 is critical in maintaining mitochondrial Complex II assembly, lipid oxidation, and insulin sensitivity, suggesting a mechanistic link between mitochondrial morphology and skeletal muscle metabolism, which is clinically relevant in combatting metabolic-related diseases.</p>
Carbon action MULTA Finnish carbon sequestration experimental field dataset 2023
<p>This dataset includes the fifth and final year observations of the Finnish Carbon Action carbon sequestration experiment. In the experiment c.a. 100 farms test carbon farming methods on a test field and an adjacent control plot. Of these 20 farms were chosen for continued sampling 2019-2023 combining field observations and soil sampling. This data will be coupled with satellite imagery, field sensors and carbon cycle modeling to forecast carbon sequestration. Subsequent sampling will track changes in soil carbon stock and soil health.</p> <p>This dataset is an update of the final monitoring year and includes summaries of the previous year measurements. The full measurements for previous years can be found in: https://zenodo.org/communities/carbonaction/ .</p> <p>The 2023 update includes data on:</p> <ul> <li>physical quality: soil structure (VESS), bulk density, porosity, water holding capacity, infiltration rate</li> <li>biological properties: earthworm counts, above ground biomass, plant cover (%), chlorophyll, microbial activity, root biomass</li> <li>chemical properties: soil nutrients, change in nutrient pools over time</li> </ul> <p> </p>
Carbon action MULTA Finnish carbon sequestration experimental field dataset 2021
<p>This dataset includes the third year observations of the Finnish Carbon Action carbon sequestration experiment. In the experiment c.a. 100 farms test carbon farming methods on a test field and an adjacent control plot. Of these 20 farms were chosen for continued sampling 2020-2024 combining field observations and soil sampling. This data will be coupled with satellite imagery, field sensors and carbon cycle modeling to forecast carbon sequestration. Subsequent sampling will track changes in soil carbon stock and soil productivity.</p> <p>The dataset will be updated annually, updates are published as separate datasets in https://zenodo.org/communities/carbonaction/ . The 2021 update includes data on:</p> <ul> <li>physical quality: soil structure (VESS), bulk density, porosity, water holding capacity, infiltration rate, aggregate stability</li> <li>biological properties: earthworm counts, above ground biomass, plant cover (%), chorophyll, microbial activity Solvita CO2 burst</li> <li>chemical properties: change in soil nutrients 2019 to 2021, change in OM </li> </ul> <p>This updated version includes the change in soil nutrients and updated soil infiltration rates as the soil texture was determined more accurately. In addition the biomass calculation was updated to include the row spacing of certain crops (12,5 cm). </p>
Carbon action MULTA Finnish carbon sequestration experimental field dataset 2020
<p>This dataset includes the second year observations of the Finnish Carbon Action carbon sequestration experiment. In the experiment c.a. 100 farms test carbon farming methods on a test field and an adjacent control plot. Of these 20 farms were chosen for continued sampling 2020-2024 combining field observations and soil sampling. This data will be coupled with satellite imagery, field sensors and carbon cycle modeling to forecast carbon sequestration. Subsequent sampling will track changes in soil carbon stock and soil productivity.</p> <p>The dataset will be updated annually, updates are published as separate datasets in https://zenodo.org/communities/carbonaction/ . The 2020 update includes data on:</p> <ul> <li>physical quality: soil structure (VESS), bulk density, porosity, water holding capacity, infiltration rate</li> <li>biological properties: earthworm counts, above ground biomass, plant cover (%), Solvita CO2 burst microbial activity</li> </ul> <p>Compared to the previous release, the biomass amounts were updated to take into account the row spacing (12,5 cm). </p>
Carbon action MULTA Finnish carbon sequestration experimental field dataset 2022
<p>This dataset includes the third year observations of the Finnish Carbon Action carbon sequestration experiment. In the experiment c.a. 100 farms test carbon farming methods on a test field and an adjacent control plot. Of these 20 farms were chosen for continued sampling 2020-2024 combining field observations and soil sampling. This data will be coupled with satellite imagery, field sensors and carbon cycle modeling to forecast carbon sequestration. Subsequent sampling will track changes in soil carbon stock and soil productivity.</p> <p>The dataset will be updated annually, updates are published as separate datasets in https://zenodo.org/communities/carbonaction/ . The 2022 update includes data on:</p> <ul> <li>physical quality: soil structure (VESS), bulk density, porosity, water holding capacity, infiltration rate</li> <li>biological properties: earthworm counts, above ground biomass, plant cover (%), chlorophyll, microbial activity (Solvita)</li> <li>chemical properties: soil nitrogen pools</li> </ul> <p>Compared to the previous version, the updated version has a corrected calculation for biomass, to take into account row spacing (12,5 cm). </p> <p>Site photos and updated water infiltration rates will be published in an updated release in winter 2024.</p>
Replication Package for "Beyond Words: On Large Language Models Actionability in Mission-Critical Risk Analysis"
<h1>Replication Package for the Paper: “Beyond Words: On Large Language Models Actionability in Mission-Critical Risk Analysis”</h1> <p>This replication package includes the raw data, questionnaire answers, and a Python notebook needed for reproducing the results detailed in the paper titled “Beyond Words: On Large Language Models Actionability in Mission-Critical Risk Analysis.”</p> <h2><a></a>Repository Structure</h2> <ol> <li><strong>Scenarios:</strong> Contains an Excel file encompassing all 141 scenarios collected (in Italian).</li> <li><strong>Training and Validation Messages:</strong> Includes the jsonl files necessary for fine-tuning the model.</li> <li><strong>Testing Messages and Ground Truth:</strong> Contains the messages utilized for testing the models.</li> <li><strong>Results:</strong> Contains Excel files with the responses from the 2 human experts and the 5 model as well as the review of the 3 human reviewer.</li> <li><strong>Tables:</strong> Contains the full Wilcoxon Test Results for H01 and H02 as well as the raw RQs results.</li> </ol> <h2><a></a>Replication Process</h2> <p>To replicate the results of our study, open the provided Python Notebook in Google Colab and follow the instructions to seamlessly reproduce the results.</p> <h1><a></a>Instructions for Use</h1> <p>To utilize this replicability package, refer to the steps outlined in the notebook file.</p> <h1><a></a>Remarks</h1> <p>If you encounter any issues or have any questions, please reach out to the authors of the paper. We will be glad to assist you!</p>
Data for "Willingness of rural and urban citizens to undertake pollinator conservation actions across three contrasting European countries"
<p>Data for: "Willingness of rural and urban citizens to undertake pollinator conservation actions across three contrasting European countries" by Costanza Geppert, Cristiano Franceschinis, Thijs P.M. Fijen, David Kleijn, Jeroen Scheper, Ingolf Steffan-Dewenter, Mara Thiene, Lorenzo Marini (2024) <em>People and Nature</em>. This dataset was obtained by administering an online questionnaire in Germany, Italy, and the Netherlands.</p>
Efficient globin production during terminal erythropoiesis depends on the synergistic action of TENT5C poly(A) polymerase and LARP4/5
<p><span><span>Red blood cell development is a unique process </span><span>where</span> <span>reduced</span> <span>transcriptome and proteome complexity</span> <span>facilitate</span><span>s</span><span> vast</span> <span>hemoglobin</span><span> production</span><span>. Here, we describe the cooperative role of cytoplasmic poly(A) polymerase </span><span>TENT5C </span><span>and the poly(A) tail-protecting </span><span>LARP4</span><span>/5 RNA-binding proteins in ensuring proper </span><span>hemoglobin production</span><span>. </span><span>TENT5C </span><span>catalytic mutant </span><span>knock-in </span><span>mice</span><span> display microcytic hypochromic anemia </span><span>resembling</span><span> constitutive knockout</span><span>. </span><span>TENT5C </span><span>counteracts gradual globin mRNA </span><span>deadenylation</span> <span>during</span><span> erythropoiesis. </span><span>In the late stages, TENT5C</span><span> dysfunction leads to globin poly(A) tail shortening and a </span><span>drastic reduction of mRNA</span> <span>level</span><span>s</span> <span>in reticulocytes. </span><span>P</span><span>roteomic experiments revealed </span><span>transient </span><span>but</span><span> specific</span><span> association of TENT5C with </span><span>LARP4</span><span>/5</span><span>. </span><span>Indeed</span><span>,</span><span> LARP</span><span>4/</span><span>5</span> <span>depletion </span><span>leads to downregulation and poly(A) tail shortening of globin mRNAs</span><span>.</span><span> Furthermore</span><span>,</span> <span>lack of TENT5C catalytic activity </span><span>is accompanied by </span><span>compensatory </span><span>upregulation </span><span>of </span><span>LARP4</span><span>/</span><span>5. </span><span>F</span><span>i</span><span>nally</span><span>, the</span><span> importance of</span><span> precise regulation of globin poly(A) tails </span><span>by </span><span>deadenylation</span><span> and re-adenylation </span><span>is highlighted by the destabilization of TENT5C </span><span>by</span><span> CCR4-</span><span>NOT </span><span>deadenylase</span><span> complex-associated </span><span>E3 </span><span>ubiquitin ligase </span><span>C</span><span>NOT4.</span></span><span> <br><br>This repository contains supplementary data accompanying related publication. The uploaded files contain following data:<br><br></span></p> <div> <p><span><strong><span>Supplementary </span><span>Data </span></strong><span><strong>1</strong> </span></span><span><span>DRS of E14.5 fetal livers, </span><span>poly(A) </span><span>profiling </span><span>stat</span><span>istics.</span></span><span> </span></p> </div> <div> <p><span><strong><span>Supplementary </span><span>Data </span><span>2</span></strong> </span><span><span>(A) All </span><span>identified</span><span> proteins </span><span>in the</span> <span>TurboID</span><span> assay </span><span>(B) Overlap between 3 replicates (C) Statistically significant GO terms for overlapping proteins with average specificity > 0.</span></span><span> </span></p> </div> <div> <p><span><strong><span>Supplementary </span><span>Data</span> <span>3</span></strong> </span><span><span>(A) Overview of used input and method details used in </span><span>degronopedia</span><span> analysis (B) Identified degron motifs (C) Degron conservation (D) Evolutionary conservation of TENT5C aa sequence scored by </span><span>ConSurf</span><span> (The table shows the residue variety in % for each position in the query sequence, each column shows the % for that amino acid found in position in the MSA)</span><span>.</span></span><span> </span></p> </div> <div> <p><span><strong><span>Supplementary </span><span>Data </span></strong><span><strong>4</strong> </span></span><span><span>Key resources table</span><span>.</span></span><span> </span></p> </div> <div> <p><span><span><strong>Supplementary Data 5</strong> </span></span><span><span>Oligonucleotides </span><span>used</span><span> in the study</span><span>.</span></span><span> </span></p> </div> <div> <p><strong><span><span>Supplementary Resource</span><span> 1</span> </span></strong><span><span>TurboID</span><span> assay</span></span> <span><span>MS/MS raw data</span><span>, each file in the</span><span> archive</span><span> corresponds to a </span><span>biological replicate of either WT (control) or </span></span><span><span>Tent5c-EGFP</span><span>.</span></span><span> </span></p> </div> <p><span> </span></p>
Data about upper limb kinematics to test the efficacy of Action Observation Treatment
<p>The dataset contains the upper-limb kinematics collected in 40 participants before and after a short-term immobilization of the right arm. Three reach-to-grasp movements were required, targeting an object placed a) frontally at the level of the shoulders (A_low), b) frontally above the head (A_high), or c) laterally at the level of the shoulders (L_low). These movements were intended to test different degrees of freedom of the shoulder joint.</p> <p>Half of the participants underwent a virtual-reality action observation treatment (see Rizzolatti et al, Neuroscience and Biobehabioral Reviews, 2021), while the control group underwent a non-motor VR stimulation.</p>
Outcome of the 3 Impact Workshops held by the ReMO COST Action between 21st and 28th January 2021
<p>These documents are the result of a series of three online workshops held by the Researcher Mental Health (ReMO) COST Action that focussed on the specific impact priorities of each of ReMO's three Working Groups:</p> <ul> <li> <p>Working Group 1: System level - the ERA working environment</p> </li> <li> <p>Working Group 2: Institutional level - Wellbeing Practices in Research Institutions</p> </li> <li> <p>Working Group 3: Local actors - Promoting researcher well-being on a practical level </p> </li> </ul> <p>These three-hours long impact workshops were based on the outcomes of a previous process to perform <a href="https://zenodo.org/record/5643588">SWOT analyses</a> and followed the guidelines and methodology that the ReMO community adapted from the "Impact+ Exercise” that was originally developed for European Commissions’ Erasmus plus projects (<a href="https://www.erasmusplus.org.uk/impact-and-evaluation">https://www.erasmusplus.org.uk/impact-and-evaluation</a>). In these workshops the following procedure was executed:</p> <ul> <li> <p>First, participants established their impact targets for the ReMO network: the <strong>network </strong>(itself), the <strong>researcher</strong>, <strong>stakeholders</strong>, and the research <strong>system</strong>. These targets were derived from the methodological guidelines of the “Impact+ Exercise”, but participants tailored them to the context of ReMO. </p> </li> <li> <p>Participants, randomly assigned to small discussion rounds for brainstorming, identified the most important issues and topics when it comes to researcher mental health and well-being. They also associated those issues and topics with the impact targets.</p> </li> <li> <p>Outcomes were discussed in a plenary session with duplications and similar items (issues and topics) being merged.</p> </li> <li> <p>Participants went through a voting procedure. Every participant could distribute three votes across all topics and issues identified by all participants. Votes were counted and topics were ranked based on the number of votes they received.</p> </li> <li> <p>Participants discussed the ranking and generated an action plan for the ReMO Action, which also served as a guide for research and intervention development to improve researcher mental health.</p> </li> <li> <p>As a result, the three Impact matrices for the three working groups were finalized and synthesized to a project level Impact matrix. </p> </li> </ul> <p>The process aimed to distill a number of key themes and actions, which are critical for developing a model of doctoral education and more general research culture embracing researcher mental health as a key priority. This process led to the publication of the <a href="https://zenodo.org/record/5559806">Researcher Mental Health and Well-being Manifesto</a>.</p> <p>The audience’s responses were anonymous, and they were recorded in a padlet, whose contents are available here.</p>
Outcome of the 3 Manifesto Development Workshops held by the ReMO COST Action between 20th and 26th April 2021
<p>These documents are the result of a series of three online workshops held between 20th and 26th April 2021 by the Researcher Mental Health (ReMO) COST Action. The 2-hour long workshops focussed on engaging the ReMO community through developing recommendations for the <a href="https://zenodo.org/record/5559806">Researcher Mental Health and Well-being Manifesto</a> based on the specific impact priorities of each of ReMO's three Working Groups:</p> <ul> <li> <p>Working Group 1: System level - the ERA working environment</p> </li> <li> <p>Working Group 2: Institutional level - Wellbeing Practices in Research Institutions</p> </li> <li> <p>Working Group 3: Local actors - Promoting researcher well-being on a practical level </p> </li> </ul> <p>These workshops were based on the outcomes of a previous process to perform <a href="https://zenodo.org/record/5643588">SWOT analyses</a> and <a href="https://zenodo.org/record/5643362">Impact analyses</a>.The following procedure was executed:</p> <ul> <li> <p>First, the working group chairs introduced the focus of each working group.</p> </li> <li> <p>Participants were randomly assigned to small parallel discussion groups. In four discussion rounds, participants identified actions to prioritise within the Manifesto and the agenda of the Working Group over project lifetime and populated the matrix of the padlet. </p> </li> <li> <p>Subsequently, through a plenary discussion the ideas recorded in the matrix were discussed, duplications and similar items were merged. </p> </li> <li> <p>As a result, the three Manifesto matrices for the three working groups were finalized and synthesized to a project level Manifesto matrix.</p> </li> </ul> <p>The process aimed to distill a number of key themes and actions, which are critical for developing a model of doctoral education and more general research culture embracing researcher mental health as a key priority. This process led to the publication of the <a href="https://zenodo.org/record/5559806">Researcher Mental Health and Well-being Manifesto</a>.</p> <p>The audience’s responses were anonymous, and they were recorded in a padlet, whose contents are available here.</p>
Mechanism of Praziquantel action at a parasitic flatworm ion channel
<p>Data supporting Park <em>et al.</em> [Mechanism of praziquantel action at a parasitic flatworm ion channel]</p> <p> </p>
Pulsation-driven mass loss observed in action from ground and space
<p>It is believed that pulsation plays a substantial role in the mass loss mechanism operating in Be stars, acting on top of rotation to lift material off the photosphere to feed the circumstellar disks. The causal relation between the combination of different non-radial pulsation modes and mass ejection is already well established. The next step forward will be given when the ejecta, which are the building blocks of the disks, are studied to determine their mass and angular content. To address this question, one must combine observations that probe the photospheric changes (photometry and spectroscopy of photospheric lines) with observations that track the ejecta proper (spectroscopy of emission lines). We are analyzing TESS data for what is by far the largest sample of Be stars observed from space to study their pulsational properties and circumstellar variability. This effort is aided by years of ground-based photometry, archival spectroscopy, and, most importantly, a campaign that is acquiring time-series Echelle spectra simultaneous with TESS observations. With the contemporaneous spectra, we are measuring changes in the photosphere and the evolution of the circumstellar environment at the same time, and establishing links between stellar and circumstellar variability. We find dramatic changes in the spectral features that probe the very inner regions of the disk on timescales of hours and days, which are correlated with signals seen by TESS when stars are actively ejecting material. Studying the star-disk interface in this way is of fundamental importance in understanding the elusive mass ejection mechanism of Be stars.</p>
Dynamic targeting enables domain-general inhibitory control over action and thought by the prefrontal cortex (data & code)
<p><strong>Data and code for:</strong></p> <p>Apšvalka, D., Ferreira, C. S., Schmitz, T. W., Rowe, J. B., & Anderson, M. C. (2022). Dynamic targeting enables domain-general inhibitory control over action and thought by the prefrontal cortex. <em>Nature Communications, </em> <strong>13, </strong>274<em>.</em> <a href="https://doi.org/10.1038/s41467-021-27926-w"> https://doi.org/10.1038/s41467-021-27926-w</a></p> <blockquote> <p>Over the last two decades, inhibitory control has featured prominently in accounts of how humans and other organisms regulate their behaviour and thought. Previous work on how the brain stops actions and thoughts, however, has emphasised distinct prefrontal regions supporting these functions, suggesting domain-specific mechanisms. Here we show that stopping actions and thoughts recruits common regions in the right dorsolateral and ventrolateral prefrontal cortex to suppress diverse content, via dynamic targeting. Within each region, classifiers trained to distinguish action-stopping from action-execution also identify when people are suppressing their thoughts (and vice versa). Effective connectivity analysis reveals that both prefrontal regions contribute to action and thought stopping by targeting the motor cortex or the hippocampus, depending on the goal, to suppress their task-specific activity. These findings support the existence of a domain-general system that underlies inhibitory control and establish Dynamic Targeting as a mechanism enabling this ability.</p> </blockquote>
Building consensus for ambitious climate action through the World Climate Simulation
<p>Sociopolitical values are an important driver of climate change beliefs, attitudes, and policy preferences. People with 'individualist-hierarchical' values favor individual freedom, competition, and clearly defined social hierarchies, while communitarian-egalitarians value interdependence and equality across gender, age, heritage, and ethnicity. In the US, individualist-hierarchs generally perceive less risk from climate change and express lower support for actions to mitigate it than communitarian-egalitarians. Exposure to scientific information does little to change these views. Here, we ask if a widely-used experiential simulation, World Climate, can help overcome these barriers. World Climate combines an engaging role-play with an interactive computer model of the climate system. We examine pre- and post-World Climate survey responses from 2,080 participants in the US and use a general linear mixed model approach to analyze interactions among participants' sociopolitical values and gains in climate change knowledge, affect, and intent to take action. As expected, prior to the simulation, participants holding individualist-hierarchical values had lower levels of climate change knowledge, felt less urgency, and expressed lower intent to act than those holding communitarian-egalitarian values. However, individualist-hierarchs made significantly larger gains across all constructs, particularly urgency, than communitarian-egalitarians. Participants' sociopolitical values also shifted: those with individualistic-hierarchical values before the simulation showed a substantial, statistically significant shift toward a communitarian-egalitarian worldview. Simulation-based experiences like World Climate may help reduce polarization and build consensus towards science-based climate action.</p>
Project files provided as supporting information to the manuscript "Coarse-grained Mori-Zwanzig dynamics in a time-non-local stationary-action framework"
<p><strong>Project files provided as supporting information to the manuscript "Coarse-grained Mori-Zwanzig dynamics in a time-non-local stationary-action framework"</strong></p> <p><br> GLE Optimization: Optimizator of GLE parameters. Uses Matlab</p> <p>MD_GLE: CG GLE and LE simulator. Uses Matlab</p> <p>Water_simulation: Folders for atomistic water system simulation with GROMACS. It requires to be run on Linux with GROMACS <br> and VOTCA packages installed.</p>
Porting the WAVEWATCH III Wave Action Source Terms to GPU - WaveWatchIII configuration files
<p>This dataset contains the mesh and model configuration information for a WaveWatchIII run using a global ustructured grid.</p> <ul> <li>global_refined_59K.msh <ul> <li>Unstructured mesh file in gmsh format. An unstructured mesh of 1 degree global resolution and 0.25 degree in regions with depth less than 4km e.g. 1 degree at the equator and 0.25 degree at the coastal regions </li> </ul> </li> <li>global_refined_228K.msh <ul> <li>Unstructured mesh file in gmsh format. An unstructured mesh of 0.5 degree global resolution and 0.125 degree in regions with depth less than 4km.</li> </ul> </li> <li>ww3_grid.inp <ul> <li>The input file for the ww3_grid pre-processing program. This file contains many of the model configuration settings.</li> </ul> </li> <li>ww3_shel.inp <ul> <li>Input file for the ww3_shel program.</li> </ul> </li> </ul>
Motor performance in violin bowing: Effects of attentional focus on acoustical, physiological and physical parameters of a sound-producing action
<p>Violin bowing is a specialised sound-producing action, which may be affected by psychological performance techniques. In sport, attentional focus impacts motor performance, but limited evidence for this exists in music. We investigated the effects of attentional focus on acoustical, physiological, and physical parameters of violin bowing in experienced and novice violinists. Attentional focus significantly affected spectral centroid, bow contact point consistency, shoulder muscle activity, and novices’ violin sway. Performance was most improved when focusing on tactile sensations through the bow (somatic focus), compared to sound (external focus) or arm movement (internal focus). Implications for motor performance theory and pedagogy are discussed.</p>
Effects of Attentional Focus on Motor Performance and Physiology in a Slow-Motion Violin Bow-Control Task: Evidence for the Constrained Action Hypothesis in Bowed String Technique
<p>The constrained action hypothesis states that focusing attention on action outcomes rather than body movement improves motor performance. Dexterity of motor control is key to successful music performance, making this a highly relevant topic to music education. We investigated effects of focus of attention (FOA) on motor skill performance and EMG muscle activity in a violin bowing task among experienced and novice upper strings players. Following a pedagogically informed exercise, participants attempted to produce single oscillations of the string at a time under three FOA: internal (on arm movement), external (on sound produced), and somatic (on string resistance). Experienced players’ number of bow slips was significantly reduced under somatic focus relative to internal, although number of successful oscillations was not affected. Triceps electromyographic activity was also significantly lower in somatic compared to internal foci for both expertise groups, consistent with physiological understandings of FOA effects. Participants’ reported thoughts during the experiment provided insight into whether aspects of constrained action may be evident in performers’ conscious thinking. These results provide novel support for the constrained action hypothesis in violin bow control, suggesting a somatic FOA as a promising performance-enhancing strategy for bowed string technique.</p>
Data for: Actions of Parathyroid hormone ligand analogs in humanized PTH1R knock-in mice
<p>Rodent models are commonly used to evaluate parathyroid hormone (PTH) and PTH-related protein (PTHrP) ligands and analogs for their pharmacologic activities and potential therapeutic utility towards diseases of bone and mineral ion metabolism. Divergence, however, in the amino acid sequences of rodent and human PTH receptors (rat and mouse PTH1Rs are 91% identical to the human PTH1R) can lead to differences in receptor-binding and signaling potencies for such ligands when assessed on rodent versus human PTH1Rs, as shown by cell-based assays in vitro. This introduces an element of uncertainty in the accuracy of rodent models for performing such pre-clinical evaluations. To overcome this potential uncertainty, we used a homologous recombination-based knock-in (KI) approach to generate a mouse (in host strain C57Bl/6N) in which cDNA encoding the human PTH1R replaces a segment (Exon 4) of the murine PTH1R gene such that the human and not the mouse PTH1R protein is expressed. Expression is directed by the endogenous mouse promoter and hence occurs in all biologically relevant cells and tissues and at appropriate levels. The resulting homozygous hPTH1R-KI (humanized) mice were healthy over at least ten generations and showed functional responses to injected PTH analog peptides that are consistent with a fully functional human PTH1R in target bone and kidney cells. The initial evaluation of these mice and their potential utility for predicting behavior of PTH analogs in humans is reported here. </p>
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.