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7,849 results for “Conservancy”
Data supplementing Lichtenberg et al. (2023) Differential effects of soil conservation practices on arthropods and crop yields. Journal of Applied Entomology
<p>This dataset contains data and scripts that supplement the publication</p> <p>Lichtenberg et al. Differential effects of soil conservation practices on arthropods and crop yields. J. Appl. Entomol. 147: 931-940. <a href="https://doi.org/10.1111/jen.13188">https://doi.org/10.1111/jen.13188</a></p> <p>Please cite the above article if you use any of the included data or code.</p> <p>Files are described in README.md.</p>
Genomic diversity and population structure of teosintes (Zea spp.) and its conservation implications
<p>The wild species of the genus <em>Zea</em> commonly named teosintes, comprise nine different taxa, distributed from northern Mexico to Costa Rica. Although this genus of plants has been extensively studied from a morphological, ecogeographical and genetic point of view, most contributions have been limited to the study of a few populations and taxa. To understand the great variability that exists between and within teosinte species, it is necessary to include the vast majority of known populations. In this context, the objective of this work was to evaluate the diversity and genomic structure of 276 teosinte populations. Molecular analyzes were performed with 3,604 plants and with data from 33,929 SNPs. The levels of genetic diversity by taxonomic group show a marked difference between species, races and sections, where the highest values of genomic diversity were found in ssp. <em>parviglumis</em> and ssp. <em>mexicana</em>. The lower values were obtained for the <em>Luxuriantes</em> section as well as ssp. <em>huehuetenagensis</em> of the section <em>Zea</em>. The results of the structure show that there is a great genetic differentiation in all the taxonomic groups considered. For ssp. <em>parviglumis</em> and <em>mexicana</em>, which are the taxa with the largest number of populations, a marked genomic differentiation was found that is consistent with their geographic distribution patterns. These results showed a loss of diversity in several teosinte populations, making a strong case for further collection, and ex situ and in situ conservation. Also, this study highlights the importance of integrating genomic diversity and structure for the applications of conservation and management.</p>
Identifying Mozambique's most critical areas for plant conservation: An evaluation of protected areas and important plant areas
<p>Successful protected area networks must represent biodiversity across taxonomic groups. However, too often plant species are overlooked in conservation planning, and the resulting protected areas may, as a result, fail to encompass the most important sites for plant diversity. The Mozambique Tropical Important Plant Areas project sought to promote the conservation of Mozambique's flora through the identification of Important Plant Areas (IPAs). Here, we use the Weighted Endemism including Global Endangerment (WEGE) index to identify the richest areas for rare and endemic plants in Mozambique and subsequently evaluate how well represented these hotspots are within the current protected area and IPA networks. We also examine the congruence between IPA and protected areas to identify opportunities for strengthening the conservation of plants in Mozambique. We found that high WEGE scores, representing areas rich in endemic/near‐endemic and threatened species, predict the presence of IPAs in Mozambique, but do not predict the presence of protected areas. We also find that there is limited overlap between IPAs and protected areas in Mozambique. We demonstrate how IPAs could be an important tool for ensuring priority sites for plant diversity are included within protected area network expansions, particularly following the adoption of the "30 by 30" target agreed within the post‐2020 Convention on Biological Diversity framework, with great potential for this method to be replicated elsewhere in the global tropics.</p>
Data from: Conservation genomics of the threatened Trispot Darter (Etheostoma trisella)
<p>The southeastern United States is a hotspot of biodiversity, but aquatic habitats are fragmented by anthropogenic activities such as hydrologic alteration. Small-stream-inhabiting fish can suffer population declines, loss of genetic diversity, and migration impediment from riverine impoundment. The Trispot Darter (<em>Etheostoma trisella</em>) is a small, freshwater fish endemic to the southeastern United States. <em>E. trisella </em>was previously believed to be extinct and has now been listed as threatened under the U.S. Endangered Species Act, due to destruction of its limited habitat and a restricted range. We used mitochondrial DNA (NADH dehydrogenase subunit 2 gene), seven microsatellite loci, and 9732 single nucleotide polymorphisms (SNPs) to evaluate population structure and diversity in <em>E. trisella</em>. Mitochondrial data provided evidence of historical connectivity between populations, with haplotype sharing across populations and weak support for population structure. Microsatellite and SNP data, however, indicate that populations have more recently become isolated. Furthermore, we detect three distinct management units (i.e., genetic groups) which reflect isolated geographic localities (i.e., Little Canoe Creek, Ballplay Creek, and a system including the Conasauga and Coosawattee rivers). We also detected a recent bottleneck event in the Ballplay Creek population as well as a low effective population size. Tests of isolation by distance further suggest that populations are structured by riverine isolation rather than geographic distance. A better understanding about the distribution, abundance, and habitat occupancy of all E. trisella populations will be important for informing future decisions for conservation of the species.</p>
Kenai Mountains to Sea: Using Thermal Infrared Imagery to Implement Long-Term Salmon Conservation - thermal imagery data set
<p>Cook Inletkeeper contracted NV5 Geospatial (formerly, Quantum Spatial Incorporated) to collect thermal infrared (TIR) during the summer of 2020 along four streams on the Kenai Peninsula in southern Alaska: Beaver Creek, Crooked Creek, Funny River, and Moose River under a project name “Kenai Rivers”. All streams were contracted to be flown in the summer of 2020 and during the afternoon hours in order to maximize the thermal contrast between the river’s water and the banks. The survey extends for a total length of 59.1 km miles of the Kenai Rivers. The Data were collected to aid the Cook Inletkeeper team to identify the spatial variability in surface temperatures as well as thermal influence of point sources, tributaries, and surface springs. The data will also be used to identify high-value habitats for the salmonids population within the four streams.</p> <p>Note: These data and related items of information have not been formally disseminated by NOAA and do not represent any agency determination, view, or policy.</p> <p>Funding for this project came, in part, from the Alaska Sustainable Salmon Fund (AKSSF Project #53003).</p>
Data from: Single-cell analysis identifies conserved features of immune dysfunction in simulated microgravity and spaceflight
<p>3-dimensional super-resolution microscopy volumes of human PBMCs recorded on a Zeiss LSM980 Airyscan2 laser scanning confocal microscope.</p> <p>Sample preparation and image capture:</p> <p>Live PBMCs were stained with 60 nM MitoTracker Red-CMX-Ros (ThermoFisher, Waltham, MA) either in 6-well plates or in the microgravity chambers for the last 2 hr of the microgravity simulation. At the end of the microgravity simulation cells were immediately fixed by 1:1 mixing the cell suspensions with 2× concentrated fixative (10% Sucrose (w/v) 120 mM KCl, 1% (w/v) glutaraldehyde, 8% (w/v) PFA pH 7.4) and incubated for 15 minutes at room temperature followed by 15 minutes on ice. Fixed cells were washed and stored in PBS until further staining for up to a week at 4 °C. 1 million fixed cells were resuspended in 1 mL of permeabilization solution (0.1% TritonX-100 in PBS) for 5 minutes. After twice washing in PBS, pellets were resuspended in 0.5 mL 1% BSA PBS containing Phalloidin-iFluor-488 (cat# ab176753, Abcam plc., Cambridge, UK) at the manufacturer’s recommended dilution, and were incubated for 90 minutes with gentle agitation. After washing in PBS, cells were stained with Hoechst 33342 (1 µg/mL in PBS) for 10 minutes. The fixed-stained cells were immobilized at 3 × 10<sup>5</sup> cells per well density in glass-bottom 96-well microplates (Greiner Bio-One, Monroe, NC), which were pre-coated with polyethyleneimine (1:15,000 (w/v)) for 16 hours in a 37 °C incubator, and washed twice with PBS. Microplates with the cell suspensions were centrifuged in a swing plate rotor centrifuge (Eppendorf 5810 R) at 400 × <em>g</em> and for 10 min and then fixed on the surface by adding an equal volume of 8% (w/v) PFA for 5 min. Finally, the fixative was replaced with 100 µL of antifade reagent (Vector Prolong Gold (ThermoFisher)). Samples were imaged immediately after this procedure on a Zeiss LSM980 Airyscan2 laser scanning confocal microscope (Carl Zeiss Microscopy, White Plains, NY). Single PBMCs were manually selected for recording based on low-resolution preview scans showing only nuclei. All singlet cells were selected in a small neighborhood to avoid biases. In each microscopy session, 24-40 cells were selected for recording in one well for each condition. This was performed in an interleaved manner, capturing 6-8 cells at a time, and then moving to the next well and then repeating this multiple times using the Experiment Designer module for automation. Super-resolution volumes of (358 × 358 × 70 pixels, 0.035 × 0.035 × 0.13 µm/voxel resolution) were recorded in the above-determined positions using Definite Focus autofocusing. A Plan-Apochromat 63 × 1.40 Oil lens, Airyscan2 SR (super-resolution) mode with optimal sampling and frame switching between 3 fluorescence channels to minimize spectral cross-bleed were used. MitoTracker Red, iFluor488, and Hoechs33342 were excited with 561, 488, and 405 nm solid-state lasers, respectively, using the optimal emission filter for each channel. 3D Airyscan2 processing was performed with standard filtering settings.</p> <p>File naming:</p> <p>Four zip files were deposited named as <Donor#id>.zip, where id goes from 1 to 4.</p> <p>Each zip file contains the following Zeiss Microscopy format image files: <Condition>_<Donor#id>_<Stain#batch>_<Cell>.czi</p> <p><Condition>:</p> <ul> <li>1G – Control culturing in 6-well plates for 25h</li> <li>uG – simulated microgravity culturing for 25h in NASA Rotating Wall Vessels</li> <li>1G+TLR – as above, with TLR 7/8 agonist (1 μM R848)</li> <li>uG+TLR– as above, with TLR 7/8 agonist (1 μM R848)</li> <li>1G+CyD– as above, with cytochalasin D</li> <li>uG+CyD– as above, with cytochalasin D</li> <li>1G+Q – as above, with quercetin 50µM</li> <li>uG+Q – as above, with quercetin 50µM</li> </ul> <p><Donor#id>: 1-4 indicates biological replicates</p> <p><Stain#batch>: 1-2 indicates experimental replicates of phalloidin staining and imaging session</p> <p><Cell>: arbitrary number to distinguish images within the same condition/donor/stain set.</p> <p>See image analysis pipelines used with these data at: https://github.com/gerencserlab/Superresolution-actin-and-mitochondria-analysis</p>
The N-terminal executioner domains of NLR immune receptors are structurally and functionally conserved across major plant lineages: Extended Data
<p>Raw data and supporting files for an updated version of the manuscript now entitled "The N-terminal executioner domains of NLR immune receptors are structurally and functionally conserved across major plant lineages".</p> <p>Related to an original version of the bioRxiv preprint: https://www.biorxiv.org/content/10.1101/2022.10.19.512840v1 </p>
Figure 1 in A review of amphidromous freshwater fishes of the Chocó biogeographical region (Colombia and Ecuador): diversity, ecology, fisheries and conservation
Figure 1. – Map of the Chocó Biogeographical Region in the Pacific of Colombia and Ecuador.
Breast-Conserving Surgery and Radiation Therapy in Patients With Multiple Ipsilateral Breast Cancer
ClinicalTrials.gov study NCT01556243. IPD Sharing: Not stated. Countries: 1. Publications: 4.
Chalazion Conservative Treatment Trial
ClinicalTrials.gov study NCT01230593. IPD Sharing: Not stated. Countries: 2. Publications: 1.
A Randomized Controlled Trial of a Conservative Fluid Balance Strategy for Patients With Sepsis and Cardiopulmonary Dysfunction (BALANCE Study)
ClinicalTrials.gov study NCT02159079. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Conservative Versus Surgical Treatment of Umbilical Pilonidal Disease
ClinicalTrials.gov study NCT01662765. IPD Sharing: NO. Countries: 1. Publications: 1.
Comparison of New Suture Anchor Technique for Bony Mallet Injury Versus Conservative Treatment
ClinicalTrials.gov study NCT03228849. IPD Sharing: NO. Countries: 1. Publications: 29.
The Conservative vs. Liberal Approach to Fluid Therapy of Septic Shock in Intensive Care Trial
ClinicalTrials.gov study NCT03668236. IPD Sharing: YES. Countries: 8. Publications: 8.
Conservative Treatment of Patients With Temporomandibular Disorders
ClinicalTrials.gov study NCT01021306. IPD Sharing: YES. Countries: 1. Publications: 5.
Early Treatment Versus Delayed Conservative Treatment of the Patent Ductus Arteriosus
ClinicalTrials.gov study NCT01958320. IPD Sharing: YES. Countries: 4. Publications: 3.
Integrating Conservative Kidney Management Options and Advance Care Planning Into a Pre-Dialysis Educational Program
ClinicalTrials.gov study NCT03229811. IPD Sharing: NO. Countries: 1. Publications: 1.
Enhancing Conservative Treatment for Urge Incontinence
ClinicalTrials.gov study NCT00223821. IPD Sharing: Not stated. Countries: 1. Publications: 2.
A Study to Assess Safety and Immunogenicity of Conserved Mosaic HIV-1 Vaccines
ClinicalTrials.gov study NCT04586673. IPD Sharing: NO. Countries: 1. Publications: 1.
Impact of Radiation Therapy on Breast Conservation in DCIS
ClinicalTrials.gov study NCT02248662. IPD Sharing: NO. Countries: 1. Publications: 13.
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.