Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
4,356
datasets available to search
ShareScore release 0.9.0
Dataset results
4,356 results for “RED”
Figure 10 in Taxonomy of Oncaeidae (Copepoda, Poecilostomatoida) from the Red Sea. V. Three species of Spinoncaea gen. nov. (ivlevi-group), with notes on zoogeographical distribution
Figure 10. Spinoncaea humesi sp. nov., female (Red Sea). (A) P1, posterior; (B) P2, posterior, intercoxal sclerite not shown; (C) P3, posterior; (D) P4, posterior, reduced proximal seta on second endopod segment arrowed.
Within- and transgenerational stress legacy effects of ocean acidification on red abalone (Haliotis rufescens) growth and survival
<p>Understanding the mechanisms by which individual organisms respond and populations adapt to global climate change is a critical challenge. The role of plasticity and acclimation, within and across generations, may be essential given the pace of change. We investigated plasticity across generations and life stages in response to ocean acidification (OA), which poses a growing threat to both wild populations and the sustainable aquaculture of shellfish. Most studies of OA on shellfish focus on acute effects, and less is known regarding the longer-term carryover effects that may manifest within or across generations. We assessed these longer-term effects in red abalone (<em>Haliotis</em> <em>rufescens</em>) using a multi-generational split-brood experiment. We spawned adults raised in ambient conditions to create offspring that we then exposed to high pCO<sub>2</sub> (1,180 μatm; simulating OA) or low pCO<sub>2</sub> (450 μatm; control or ambient conditions) during the first three months of life. We then allowed these animals to reach maturity in ambient common garden conditions for four years before returning the adults into high or low pCO<sub>2</sub> treatments for 11 months and measuring growth and reproductive potential. Early-life exposure to OA in the F1 generation decreased adult growth rate, even after 5 years, especially when abalone were re-exposed to OA as adults. Adult, but not early-life exposure, to OA negatively impacted fecundity. We then exposed the F2 offspring to high or low pCO<sub>2</sub> treatments for the first three months of life in a fully factorial, split-brood design. We found negative transgenerational effects of parental OA exposure on survival and growth of F2 offspring, in addition to significant direct effects of OA on F2 survival. These results show that the negative impacts of OA can last within and across generations, but that buffering against OA conditions at critical life-history windows can mitigate these effects.</p>
Pacing of primary murine cardiomyocytes expressing human TRPV1 using infra-red laser
<h2>Abstract</h2> <p>The expression of human TRPV1 in cardiomyocytes allowed us to induce action potentials (APs) by pulse irradiation with infra-red (IR) diod laser. Mice cardiomyocytes were transformed by AAV-based vectors bearing construction of TRPV1 with mRuby (for expression detection). We selected cells having their own intrisic AP generation and then set them up for measuring membrane potential in a current-clamp mode. Cells were irradiated using 2 or 7 Hz laser pulses. Laser trigger pulses were recorded along with cell potential. In the dataset <em><strong>05-12-22.zip</strong></em>, there are some records where electrical stimulation were used alone or together with IR pulses. Experimental conditions are summarized in the file <em><strong>experiment-descriptions.tsv</strong></em>.</p> <h2>Primary cell production and transformation</h2> <p>Experiments were carried out using C57Bl/6J mice (The Jackson Laboratory, #000664, RRID: IMSR_JAX:000664). The mixed mouse primary neonatal cardiomyocyte cell culture was obtained using a neonatal heart dissociation kit (Miltenyi Biotec, 130-098-373) according to the manufacturer’s instructions. The cells were cultured in DMEM/F12, 1:1 mixture (BioloT, 1.3.7.2.) supplemented with 10% FBS, penicillin 100 U/ml /streptomycin 100 mg/ml, and L-glutamine 0.365 g/l. The culture was seeded on 10mm coverslips coated with 10 mg/ml gelatin diluted in PBS and maintained at 37℃ in 5% CO2. For transient expression of the hTRPV1 channel, a reporter protein, and a fluorescent Ca2+ sensor GCaMP6s, we used AAV-based vectors with the encoded genes above. We used AAV-DJ serotype at a MOI of 12,000 VG/cells for cTnT_hTRPV1(sh)_P2A_mRuby based viruses, and a MOI of 2,500 VG/cells for cTnT_GCaMP6s ones. The cells were infected on the next day after plating, and the transgene expression peak was observed on the third day after the infection.</p> <h2>Distant heating system</h2> <p>The system was equipped with a fiber coupled laser diode (LD) 4PN-117 (SemiNex) as a powerful heating laser, providing radiation at a wavelength of 1375 nm with an average power of up to 4.3 W through a multimode fiber with a core diameter of 105 μm and 0.22NA. The LD was mounted onto a TEC-controlled plate “264 TEC HP LaserMount” (A.I.), which was operated by TEC driver TECSource 5305 (A.I.); current stabilization and control for LD were performed with LD driver LaserSource 4320 (A.I.). The laser was controlled via the TTL output from the HEKA EPC-10 amplifier. Different laser intensities, and pulse widths were used. Laser power, <em><strong>P</strong></em>, can be computed from trigger voltage, <em><strong>U</strong></em>, by an equation: <em><strong>P [W] = -0.28 + 1.42 * U [V]</strong></em></p> <h2>Electrophysiology of single cardiac cells </h2> <p>Patch electrodes were pulled from hard borosilicate capillary glass (Sutter Instruments flaming/brown micropipette puller) and filled with an intracellular solution consisting of (in mM) K-gluconate, 100; KCl, 40; HEPES, 10; NaCl, 8; MgATP, 4; MgGTP, 0.3; phosphocreatine, 10 (pH 7.3 with KOH). Cells were identified visually using IR-video microscopy using a Hamamatsu ORCA-Flash4.0 V3 Digital sCMOS camera (Hamamatsu Photonics) expression of wild type or mutant TRPV1 was confirmed by the presence of red flourescent protein. Coverslips were placed in a recording chamber continuously perfused with heated Tyrode's solution. Whole-cell recordings were taken at 32°C in current-clamp mode using a HEKA EPC-10 amplifier (List Elektronik) with a sampling rate of 100 μs. Steady state current was injected to achieve a membrane potential of approximately −60 to −90 mV. For experiments using the IR laser the optic fiber was placed near the cell, and light from a green laser diode was shone onto the cell to check correct positioning. </p>
Generation of Random laser from Dye-Derived Red-Emitting Carbon Dots
<p>Carbon dots are carbon-based nanoparticles renowned for their intense light-emitting capabilities covering the whole visible light range. Here, we overcome these problems by solvothermally synthesizing carbon dots starting from Neutral Red, a common red-emitting dye, as a molecular precursor. The obtained nanoparticles are highly luminescent in the red region, with a quantum yield comparable to that of the starting dye. Most importantly, the nanoparticle carbogenic matrix protects the Neutral Red molecules from photobleaching under ultraviolet excitation while preventing aggregation-induced quenching, thus allowing solid-state emission inside PVA. Finally, the dye-based carbon dots demonstrate stable and efficient random lasing emission in the red region.</p>
Reproductive state alters vocal characteristics of female North American red squirrels (Tamiasciurus hudsonicus)
<p>Female advertisement of reproductive state and receptivity has the potential to play a large role in the mating systems of many taxa, but investigations of this phenomenon are underrepresented in the literature. North American red squirrels (<em>Tamiasciurus hudsonicus</em>) are highly territorial and engage in scramble competition mating, with males converging from spatially disparate territories to engage in mating chases. Given the narrow estrus window exhibited in this species, the ubiquitous use of vocalizations to advertise territory ownership, and the high synchronicity of males arriving from distant territories, we hypothesized that female vocalizations contain cues relating to their estrous state. To test this hypothesis, we examined the spectral and temporal properties of female territorial rattle vocalizations collected from females of known reproductive condition over 3 years. While we found no distinct changes associated with estrus specifically, we did identify significant changes in the spectral characteristics of rattles relating to both female body mass and reproductive state relative to parturition. To the best of our knowledge, this is the first evidence of changes in vocal characteristics associated with late pregnancy in a non-human mammal.</p>
Figure 1 in Taxonomic review of the "posteli-species group" of goatfishes (genus Parupeneus, Mullidae), with description of a new species from the northern Red Sea
Figure 1. – Relationships among eight morphometric characters including SL and total number of gill rakers in the five posteli-group species and three populations of Parupeneus posteli.
Figure 3. – A-E in Taxonomic review of the "posteli-species group" of goatfishes (genus Parupeneus, Mullidae), with description of a new species from the northern Red Sea
Figure 3. – A-E: Parupeneus posteli; A: SAIAB 83959, 88 mm SL, Baissac Bank, southern Mascarene Plateau (O. Alvheim and D. Tweddle); B: SAIAB 83959, 90 mm SL, same locality details (O. Alvheim and D. Tweddle); C: SAIAB 83898, 105 mm SL, Soudan Bank, southern Mascarene Plateau (O. Alvheim and D. Tweddle); D: large-sized fish (SL unknown), Réunion, Mascarene Islands (P. Guézé); E: MNHN 1965-0056, HT, 150 mm SL, same locality details (MNHN); F: Parupeneus sinai n. sp., MNHN 1967-0557, HT, 83 mm SL, northern Red Sea, right side shown, image reversed (F. Uiblein). Scale bars = 20 mm.
Figure 2. – A in Taxonomic review of the "posteli-species group" of goatfishes (genus Parupeneus, Mullidae), with description of a new species from the northern Red Sea
Figure 2. – A: Parupeneus chrysonemus BPBM 34775, 129 mm SL, Midway Atoll, Hawaiian Islands (J.E. Randall); B: P. chrysonemus BPBM 39141, 140 mm SL, Oahu, Hawaiian Islands (J.E. Randall); C: P. louise, large-sized fish (SL unknown), Rurutu, Austral Islands, French Polynesia (A. Stein); D: P. moffiti, HT, 230 mm SL, Guam, Mariana Islands (R.F. Myers). Scale bars = 20 mm.
Red vision in animals is broadly associated with lighting environment but not types of visual task
<p>Red sensitivity is the exception rather than the norm in most animal groups. Among species with a long wavelength sensitive (LWS) photoreceptor, peak wavelength sensitivity (λ<sub>max</sub>) varies substantially and it is unclear whether this variation can be explained by visual tuning to the light environment or to visual tasks such as signalling or foraging. Here, we examine long wavelength sensitivity across a broad range of taxa showing diversity in LWS photoreceptor λ<sub>max</sub>: insects, crustaceans, arachnids, amphibians, reptiles, fish, sharks and rays. We identified 161 species with a LWS photoreceptor (λ<sub>max</sub> ≥ 550 nm). We found evidence supporting visual tuning to the light environment: terrestrial species had longer λ<sub>max</sub> than aquatic species, and of these, species from turbid shallow waters had longer λmax than those from clear or deep waters. Of the terrestrial species, diurnal species had longer λ<sub>max</sub> than nocturnal species, but we did not detect any differences across terrestrial habitats (closed, intermediate or open). We found no association with proxies for visual tasks such as having red morphological features or utilising flowers or coral reefs. These results support the emerging consensus that, in general, visual systems are adapted to broad range of tasks, rather than tuned to certain tasks.</p>
Fig. 5. Trypanoxyuris kotudoi n in Pinworms of the red howler monkey (Alouatta seniculus) in Colombia: Gathering the pieces of the pinworm-primate puzzle
Fig. 5. Trypanoxyuris kotudoi n. sp. (A) Male full body, lateral view. (B) Male cephalic end, apical view. (C) Male posterior end, ventral view; (D) Male posterior end, lateral view, (E) Female full body, lateral view; (F) Female cephalic end, apical view; (G) Female cross section showing lateral alae; (H) Egg.
Fig. 6 in Pinworms of the red howler monkey (Alouatta seniculus) in Colombia: Gathering the pieces of the pinworm-primate puzzle
Fig. 6. SEM of buccal structures of males of Trypanoxyuris species found in howler monkeys (A) Trypanoxyuris seunimii n. sp. (B) T. keumimae n. sp. (C) T. kotudoi n. sp. (D) T. minutus from Alouatta seniculus. (E) T. pigrae. (F) T. minutus from Mesoamerican howler monkeys. R: right ventral lip; L: left ventral lip. White arrow pointing at the sharp protuberances formed as a result of the notches in the lips.
Fig. 4 in Pinworms of the red howler monkey (Alouatta seniculus) in Colombia: Gathering the pieces of the pinworm-primate puzzle
Fig. 4. SEM of buccal structures of females of Trypanoxyuris species found in howler monkeys (A) Trypanoxyuris seunimii n. sp. (B) T. keumimae n. sp. (C) T. kotudoi n. sp. (D) T. minutus from Alouatta seniculus. (E) T. pigrae. (F) T. minutus from Mesoamerican howler monkeys. R: right ventral lip; L: left ventral lip. White arrow pointing at the lateral indentations. Black arrow pointing at the square-shaped edge.
Fig. 3. Trypanoxyuris keumimae n in Pinworms of the red howler monkey (Alouatta seniculus) in Colombia: Gathering the pieces of the pinworm-primate puzzle
Fig. 3. Trypanoxyuris keumimae n. sp. (A) Female full body, lateral view. (B) Female cephalic end, apical view. (C) Male cephalic end, apical view, (D) Male full body, lateral view; (E) Male posterior end, lateral view, (F) Male posterior end, ventral view; (G) Female cross section showing lateral alae (H) Egg.
Fig. 2. Trypanoxyuris seunimii n in Pinworms of the red howler monkey (Alouatta seniculus) in Colombia: Gathering the pieces of the pinworm-primate puzzle
Fig. 2. Trypanoxyuris seunimii n. sp. (A) Female full body, lateral view. (B) Female cephalic end, apical view. (C) Male cephalic end, apical view, (D) Female cross section showing lateral ala; (E) Egg; (F) Male full body, lateral view; (G) Male posterior end, ventral view; (H) Male posterior end, lateral view.
Data from: Among-individual behavioural variation in the ornamental red cherry shrimp, Neocaridina heteropoda
<p><span>Personality variation, defined as among-individual differences in behaviour that are repeatable across time and context, has been reported across animal taxa. From an evolutionary perspective, characterising the amount and structure of this variation is useful since differences among-individuals are the raw material for adaptive behavioural evolution. However, it is becoming apparent that behavioural variation among-individuals also has implications for more applied areas of evolution and ecology – from invasion biology, to ecotoxicology, and selective breeding in captive systems. Here, we investigate the structure of personality variation in the red cherry shrimp, <em>Neocaridina heteropoda</em>, a popular ornamental species that is readily kept and bred under lab conditions and is emerging as a decapod crustacean model across these fields, but for which basic biological, ecological, and behavioural data is limited. Using two assays and a repeated measures approach, we quantify behaviours putatively indicative of shy-bold variation and test for sexual dimorphism and/or size-dependent behaviours (as predicted by some state-dependent models of personality). We find moderate to high behavioural repeatabilities across traits. Although strong individual level correlations across behaviours are consistent with a major personality axis underlying these observed traits, the multivariate structure of personality variation does not fully match a priori expectations of a shy-bold axis. This may reflect our ecological naivety with respect to what really constitutes bolder, more risk prone, behaviour in this species. We find no evidence for sexual dimorphism and only weak support size-dependent behaviour. Our study contributes to the growing literature describing behavioural variation in aquatic invertebrates. Furthermore, it lays a foundation for further studies harnessing the potential of this emerging model system. In particular, this existing behavioural variation could be functionally linked to life-history traits and invasive success, and serve as target of artificial selection or bioassays. It thus holds significant promise in applied research across ecotoxicology, aquaculture, and invasion biology.</span></p>
Red-footed and masked boobies stable isotope data
<p>Animals that co-occur in a region (sympatry) may share the same environment (syntopy), and niche differentiation is expected among closely related species competing for resources. The masked booby (<em>Sula dactylatra</em>) and smaller congeneric red-footed booby (<em>Sula sula</em>) share breeding grounds. In addition to the inter-specific size difference, females of both species are also larger than the respective males (reversed sexual size dimorphism). Although both boobies consume similar prey, sometimes in mixed-species flocks, each species and sex may specialize in terms of their diet or foraging habitats. We examined inter- and intra-specific differences in isotopic values (δ<sup>13</sup>C and δ<sup>15</sup>N) in these pelagically feeding booby species during the incubation period at Clarion Island, Mexico, to quantify the degrees of inter- and intra-specific niche partitioning throughout the annual cycle. During incubation, both species preyed mainly on flyingfish and squid, but masked boobies had heavier food loads than red-footed boobies. There was no overlap in isotopic niches between masked and red-footed boobies during breeding (determined from whole blood), but there was a slight overlap during the non-breeding period (determined from body feathers). Female masked boobies had a higher trophic position than conspecific males during breeding; however, no such pattern was detected in red-footed boobies. These results provide evidence of inter- and intra-specific niche partitioning in these tropical seabird species, particularly during the breeding period and in the more dimorphic species. Our results suggest that these closely related species use different strategies to cope with the same tropical marine environment.</p>
FIGURE 5 in Differential expression of HPG-axis genes in autotetraploids derived from red crucian carp Carassius auratus red var., × blunt snout bream Megalobrama amblycephala,
FIGURE 5 Mean (+SD) relative expression of gnrh2, fshb, lhb, fshr and lhr messenger (m)RNA in (a) the breeding season () 2n, and () 4n and (b) the non-breeding season in Carassius auratus red var. () 2n, and () 4n. (RCC,) and autotetraploid C. auratus red var. ♀ × Megalobrama amblycephala ♂ (4nRR,). T, gene detected in the testis; O, gene detected in the ovary. *, significant difference between RCC and 4nRR (P <0.05)
FIGURE 4 Deduced amino-acid sequences for the Gnrh2 in Differential expression of HPG-axis genes in autotetraploids derived from red crucian carp Carassius auratus red var., × blunt snout bream Megalobrama amblycephala,
FIGURE 4 Deduced amino-acid sequences for the Gnrh2 () and Lhr () genes in Carassius auratus red var. (RCC) and autotetraploid C. auratus red var. ♀ × Megalobrama amblycephala ♂ (4nRR)
FIGURE 2 in Differential expression of HPG-axis genes in autotetraploids derived from red crucian carp Carassius auratus red var., × blunt snout bream Megalobrama amblycephala,
FIGURE 2 (a) The mature eggs (scale bar = 100 μm) and (b) mature sperm (scale bar = 10 μm) of autotetraploid Carrasius auratus red var. ♀ x Megalobrama amblycephala ♂ (4nRR)
FIGURE 3 in Differential expression of HPG-axis genes in autotetraploids derived from red crucian carp Carassius auratus red var., × blunt snout bream Megalobrama amblycephala,
FIGURE 3 Reverse-transcription (RT)-PCR analysis of the expression of (a) gnrh2, (b) fshb, (c) lhb, (d) fshr and (e) lhr messenger (m)RNA in various tissues of autotetraploid Carrasius auratus red var. ♀ x Megalobrama amblycephala ♂ (4nRR). The upper strip of each panel (a)–(e) shows the positive control of actin gene while the lower strip of each panel shows the RT-PCR amplification of the target gene
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.