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Fig. 1 in The Morphology and Temperature-dependent Development of Mylabris phalerata Pallas (Coleoptera: Meloidae)
Fig. 1. Developmental stages of Mylabris phalerata. A: Egg (2.4 mm); B: L1 (2.6 mm); C: L2 (5.1 mm); D: L3 (13.7 mm); E: L4 (22.4 mm); F: L5 (29.2 mm); G: Pupa (30.1 mm); H: Adult (31.2 mm).
Fig. 4 in Sperm morphology and some aspects of acrosomal complex development in four species of Heteronemertea (Pilidiophora, Nemertea)
Fig. 4. Ultrastructural aspects of the acrosomal complex development in Kulikovia alborostrata (A) Primary spermatocyte (sc) (B) Fragment of primary spermatocyte with Golgi body (Gb) located near a pair of centrioles (c). Arrows indicate small proacrosomal vesicles (C) Dividing spermatocyte (D) Higher magnification of proacrosomal vesicles (arrows) (E) Spermatid with single large rounded acrosomal vesicle (av) (F) Spermatids with transformed acrosomal vesicles in posterior and anterior positions. Abbreviations: chr, chromatin; m, mitochondrion; n, nucleus; pnf, posterior nuclear fossa. Scale bars: A, F = 2 μm, B, C, E = 1 μm, D = 0.5 μm.
Fig. 1 in Sperm morphology and some aspects of acrosomal complex development in four species of Heteronemertea (Pilidiophora, Nemertea)
Fig. 1. Fine organization of spermatozoon in Micrura bella (A–C) SEM (D–J) TEM (A) General view of spermatozoon (B) Sperm head with visible acrosomal region (a), slightly curveted nucleus (n) and midpiece (mp) with mitochondria rounded or slightly elongated along anterior/posterior axis. Flagellum (f) is posteriorly oriented (C) Longitudinal section along sperm head that consists of acrosomal complex (ac), nucleus, midpiece containing mitochondria, and centriolar complex (cc) (D) Longitudinal section along acrosomal complex containing large electron-dense vesicle in apical position (dv), thin layer of electron-dense material surrounding the electron-dense vesicle (arrows), small vesicles with moderate electron density (mv) located beneath plasma membrane and lower than the region of large vesicle; flocculent subacrosomal material of moderate electron density (asterisk) fills the central region immediately under the electron-dense vesicle (E) Higher magnification of large acrosomal vesicle in longitudinal projection. Its basal part forms invagination (F) Transverse section across acrosomal complex at the level of small vesicles. The vesicles are located peripherally forming a ring, often open. Central part is filled by subacrosomal material (asterisk) (G) Transverse section across acrosomal complex at the level of electron-dense vesicle. The vesicle is surrounded by electron-dense ring (arrow) (H) Longitudinal section along midpiece with centriolar complex represented by proximal (pc) and distal centrioles (dc) and mitochondria. The basal part of the nucleus forms posterior nuclear fossa (pnf) and invaginations hosting mitochondria (arrow) at sites of their contact with the nucleus (I) Transverse section across midpiece containing five mitochondria (J) Pericentriolar complex of distal centriole formed by satellite fibers (sf) (K) Transverse section through flagellum. Scale bars: A = 5 μm, B, C =2 μm, D-G =0.2 μm, H-J = 0.5 μm, K = 0.1 μm.
FIGURE 3. Begonia nigritarum. A. habit. B. inflorescence. C. staminate flower. D. pistillate flower. E. developing capsule. F. leaf, abaxial view. G. rhizome. Scale bars for A in Begonia ×dinglensis, a natural hybrid of Philippine Begonia section Baryandra, as evidenced by morphological, phylogenetic and cytological data
FIGURE 3. Begonia nigritarum. A. habit. B. inflorescence. C. staminate flower. D. pistillate flower. E. developing capsule. F. leaf, abaxial view. G. rhizome. Scale bars for A is 10 cm, B and G are 1 cm, C–E are 0.5 cm, F is 3 cm. [All photos from Ching-I Peng 23858 (HAST).]
FIGURE 2. Begonia camiguinensis. A. habit. B. inflorescence. C. staminate flower. D. pistillate flower. E. developing capsule. F. leaf, abaxial view. G. rhizome. Scale bars for A in Begonia ×dinglensis, a natural hybrid of Philippine Begonia section Baryandra, as evidenced by morphological, phylogenetic and cytological data
FIGURE 2. Begonia camiguinensis. A. habit. B. inflorescence. C. staminate flower. D. pistillate flower. E. developing capsule. F. leaf, abaxial view. G. rhizome. Scale bars for A is 10 cm, B and G are 1 cm, C–E are 0.5 cm, F is 3 cm. [All photos from Ching-I Peng 23853 (HAST).]
FIGURE 10 in A new species of Echinoderes (Kinorhyncha: Cyclorhagida: Echinoderidae) from the Argentinean continental shelf with notes on its postembryonic development and on subcuticular morphological characters unreported for Kinorhyncha
FIGURE 10. Echinoderes goku sp. nov., last juvenile stage moulting to female, ZMB 12267, dorsal view (A, B) and optical section (C). A. Segments 7–10. B, C. Segments 10 and 11. Abbreviations: co, sublateral, subcuticular conical structure opening on segment 10; fe, refers to female character; juv, refers to character of last juvenile stage; las, lateral accessory spine; lts, lateral terminal spine; mds, middorsal spine; ps, postmarginal spicula; re, cuticular reinforcement; s, segment; slt, sublateral tube. Digits after abbreviations indicate the corresponding segment number. Arrowheads in A mark papillate sensory spots. Scale bar in A 50 µm. Scale bar in B 30 µm, valid for B and C.
FIGURE 7 in A new species of Echinoderes (Kinorhyncha: Cyclorhagida: Echinoderidae) from the Argentinean continental shelf with notes on its postembryonic development and on subcuticular morphological characters unreported for Kinorhyncha
FIGURE 7. Echinoderes goku sp. nov., female, ZMB 12271 (A–E) and ZMB 12349 (F), right side (A–D) and dorsal view (E, F), SEM. A. Segments 1–3. B. Segments 4–6. C. Segments 7–8. D. Segments 9–11. E. Segments 10 and 11. F. Fringe of sublateral, subcuticular conical structure opening on segment 10. Abbreviations: co, fringe of sublateral, subcuticular conical structure opening on segment 10; gc1, type-1 gland cell outlet; hr, trunk cuticular hair; lat, lateral accessory tube; ltas, lateral accessory terminal spine; lts, lateral terminal spine; lvs, lateroventral spine; mds, middorsal spine; pf, primary pectinate fringe; s, segment; sdt, subdorsal tube (lost on segment 2); sf, secondary pectinate fringe; slt, sublateral to midlateral tube; te, tergal extension. Digits after abbreviations indicate the corresponding segment number. Arrowheads mark type-1 sensory spots. Scale bar in A–E 10 μm. Scale bar in F 5 µm.
FIGURE 6 in A new species of Echinoderes (Kinorhyncha: Cyclorhagida: Echinoderidae) from the Argentinean continental shelf with notes on its postembryonic development and on subcuticular morphological characters unreported for Kinorhyncha
FIGURE 6. Echinoderes goku sp. nov., female, ZMB 12271 (A, C) and ZMB 12272 (B), in laterodorsal (A), ventral (B), and dorsal-right (C) view, SEM. A. Habitus. B. Mouth cone. White arrow points to bifurcated tip of posterior spinose process. C. Two neighbouring introvert sector. Abbreviations: asp, anterior spinose process; in, introvert; ltas, lateral terminal accessory spine; lts, lateral terminal spine; lvs, lateroventral spine; lvt, lateroventral tube; mc, mouth cone; mds, middorsal spine; oos, outer oral style; psc, primary spinoscalid; psp, posterior spinose process; s, segment; sc, spinoscalid; slt, sublateral tube; tr, trichoscalid. Digits after abbreviations indicate the corresponding segment (A) or introvert ring number (C). Scale bar in A 100 μm. Scale bar in B and C 10 μm.
FIGURE 5 in A new species of Echinoderes (Kinorhyncha: Cyclorhagida: Echinoderidae) from the Argentinean continental shelf with notes on its postembryonic development and on subcuticular morphological characters unreported for Kinorhyncha
FIGURE 5. Female specimens of species of Echinoderes, dorsal view (A, B, H, I, L–N), lateral view (C, K), and optical section (D–G, J), DIC. A–E. Echinoderes goku sp. nov. A, B. paratype ZMB 12335, segments 1–5 (A) and 6–9 (B). C. Paratype ZMB 12344, segments 2–7. D, E. Paratypes ZMB 12342 (D) and ZMB 12264 (E), segments 10 and 11 (D) and 10 (E). F–N. Segments 10 and 11. F, G. Echinoderes capitatus, ZMB 11913 (F) and 11921 (G). H, I. Echinoderes meteorensis, paratypes ZMB 11571b (H) and ZMB 11572b (I). J. Echinoderes unispinosus, paratype ZMB 11589b. K, L. Echinoderes apex, holotype ZMB 11561, paratype ZMB 11562a. M. Echinoderes ferrugineus, ZMB 11929. N. Echinoderes pilosus, paralectotype SMNH 9256; black arrow marks cuticular sheath around tube. Abbreviations: co, female-specific sublateral, subcuticular conical structure; gc1, type-1 gland cell outlet; ldt, laterodorsal tube; ltas, lateral accessory terminal spine; lts, lateral terminal spine; lvs, lateroventral spine; lvt, lateroventral tube; mds, middorsal spine; re, cuticular reinforcement; s, segment; sdt, subdorsal tube; slt, sublateral tube; sph, laterodorsal subcuticular spherical structure; vlt, ventrolateral tube. Digits after abbreviations indicate the corresponding segment number. Arrowheads mark type-1 sensory spots. White arrows point to type-1 gland cell outlets. Scale bar in B 50 μm, valid for A–C. Scale bar in D 20 µm, valid for D–M. Scale bar in N 20 µm.
FIGURE 2 in A new species of Echinoderes (Kinorhyncha: Cyclorhagida: Echinoderidae) from the Argentinean continental shelf with notes on its postembryonic development and on subcuticular morphological characters unreported for Kinorhyncha
FIGURE 2. Line art illustrations of Echinoderes goku sp. nov., A, B, male holotype (MACN-In 43755), C, D, female paratype (MACN-In 43756b). A. Dorsal view. B. Ventral view. C, D. Segments 10 and 11, dorsal (C) and ventral view (D). Placids illustrated as appearing in SEM. Sublateral structures often hidden by the neighbouring body cuticle in the more or less dorsoventral mount and often only illustrated for one side. Abbreviations: co, female-specific, subcuticular conical structure; hr, cuticular hair; lat, lateral accessory tube; ltas, lateral terminal accessory spine; lts, lateral terminal spine; lvs, lateroventral spine; lvt, lateroventral tube; mdgc, middorsal gland cell outlet; mds, middorsal spine; pa, pachycycli; pe, penile spine; pl, placid; pvp, paraventral patch of cuticular hairs; sdt, subdorsal tube; slt, sublateral to midlateral tube; te, tergal extension; vlp, ventrolateral to paraventral patch of cuticular hairs; vlt, ventrolateral tube; vmss, ventromedial sensory spot. Digits after abbreviations indicate the corresponding segment number. Scale bar: 100 μm.
FIGURE 3 in A new species of Echinoderes (Kinorhyncha: Cyclorhagida: Echinoderidae) from the Argentinean continental shelf with notes on its postembryonic development and on subcuticular morphological characters unreported for Kinorhyncha
FIGURE 3. Echinoderes goku sp. nov., male paratype ZMB 12334 (A, C), juvenile non-type ZMB 12347 (B, D), and male holotype MACN-In 43755 (E–J), in ventral (A), lateral (B, D), and dorsal view (C, E–J), DIC. A–D. Habitus of specimens infected with fungus before (A, B) and after treatment with formaldehyde for two weeks (C, D). E. Segments 1–5. F, J. Segments 6–9 at different focal levels. G–I. Segments 10 (G), 10 and 11 (H), and 11 (I) at different focal levels. Abbreviations: fu, fungus; hr, trunk cuticular hair; mds, middorsal spine; pa, pachycycli; pl, placid; ppf, primary pectinate fringe; s, segment; sdt, subdorsal tube; slt, sublateral tube. Digits after abbreviations indicate the corresponding segment number. Arrowheads mark type-1 sensory spots. Arrows point to type-1 gland cell outlets. Scale bar in D 100 μm, valid for A–D. Scale bar in F 50 µm, valid for E–J.
FIGURE 9 in A new species of Echinoderes (Kinorhyncha: Cyclorhagida: Echinoderidae) from the Argentinean continental shelf with notes on its postembryonic development and on subcuticular morphological characters unreported for Kinorhyncha
FIGURE 9. Echinoderes goku sp. nov., last juvenile stage moulting to female, ZMB 12267, ventral (B–E), optical section (A, F), and dorsal side (G, H). A. Habitus. B. Segments 1–4. C, D. Segments 5–8 at different focal levels showing aspects of the juvenile (C) and adult (D) cuticle. E. Segments 8–11. F. Segments 9–11. G. Segments 1–4. H. Segments 4–7. Abbreviations: bs, button-like structures; fe, refers to female character; juv, refers to character of last juvenile stage; lat, lateral accessory tube; ltas, lateral accessory terminal spine; lts, lateral terminal spine; lvs, lateroventral spine; lvt, lateroventral tube; mds, middorsal spine; mv, midventral articulation of sternal plates; mvpl, midventral placid; ps, postmarginal spicula; s, segment; vlt, ventrolateral spine. Digits after abbreviations indicate the corresponding segment number. Scale bar in A 100 µm. Scale bar in F 50 µm, valid for B–H.
FIGURE 8 in A new species of Echinoderes (Kinorhyncha: Cyclorhagida: Echinoderidae) from the Argentinean continental shelf with notes on its postembryonic development and on subcuticular morphological characters unreported for Kinorhyncha
FIGURE 8. Echinoderes goku sp. nov., male (ZMB 12350, A) and female (ZMB 12271, B–F), right side (A) and ventral view (B–F), SEM. A. Segment 11 with penile spines. B. Segments 10–11. C. Segments 1–3. D. Segments 4–6. E. Segments 7 and 8. F. Segment 9–11. Abbreviations: dv, attachment site of dorsoventral muscle; lat, lateral accessory tube; ltas, lateral accessory terminal spine; lts, lateral terminal spine; lvs, lateroventral spine; peb, broad penile spine; pet, tubular penile spine; pf, primary pectinate fringe; pvp, paraventral patch of short cuticular hairs; s, segment; sf, secondary pectinate fringe; te, tergal extension; vlt, ventrolateral tube. Digits after abbreviations indicate the corresponding segment number. Arrowheads mark type-1 sensory spots. Scale bar in A–F 10 μm.
FIGURE 4 in A new species of Echinoderes (Kinorhyncha: Cyclorhagida: Echinoderidae) from the Argentinean continental shelf with notes on its postembryonic development and on subcuticular morphological characters unreported for Kinorhyncha
FIGURE 4. Echinoderes goku sp. nov., male holotype MACN-In 43755 (A–C), male paratype ZMB 12318 (D, F–H) and female paratypes ZMB 12337 (E), ZMB 12265 (I, K), and MACN-In 43756b (J), ventral view (A–E, I, K), dorsal view (F–H), and optical section (J), extended focus image from four images (D, K), DIC. A. Segments 1–6. B. Segments 7–10. C. Segments 9–11. D. Segments 1–4. E. Segment 10. F–H. Segments 10 and 11 at different focal levels. I, J. Segments 8–11. K. Mouth cone with outer oral styles. Abbreviations: bs, button-like structures; lat, lateral accessory tube; ltas, lateral accessory terminal spine; lts, lateral terminal spine; lvs, lateroventral spine; lvt, lateroventral tube; mvpl, midventral placid; oos, outer oral style; ov, ovarium with egg; pa, pachycycli; peb, broad penile spine; pet, tubular penile spine; pvp, paraventral patch of short cuticular hairs; re, cuticular reinforcement; s, segment; slt, sublateral tube; te, tergal extension; vlt, ventrolateral tube. Digits after abbreviations indicate the corresponding segment number. Arrowheads mark type-1 sensory spots. Arrows point to type-1 gland cell outlets. Scale bar in B 50 μm, valid for A–D, I, J. Scale bar in G 50 µm, valid for F, G. Scale bar in H 20 µm. Scale bar in K 30 µm, valid for E, K.
Granule ripples in the Kumtagh Desert, China: morphological and sedimentary characteristics, and development processes
<p>1. Observation of composition and morphology</p> <p>First, we selected typical granule ripples of different sizes and development stages at each observation site, and sampled the surface particles (to a depth of 1 cm) on the ridges of 85 granule ripples by scraping the surface with a steel ruler. The sample weight averaged 428 g (with values ranging between 249 and 697 g). We determined the grain-size distribution using a set of 15 standard sieves with a mesh size ranging from 0.0565 mm to 20 mm. We used the parameters proposed by Folk and Ward (1957) to calculate the grain size characteristics: the cumulative grain size of the 20 percentile (P20), and the average grain diameter, sorting coefficient, skewness, and kurtosis. Here, P20 represents the particle size corresponding to the cumulative distribution rate of 20% in the cumulative particle-size distribution diagram.</p> <p>Second, we defined ripple morphological characteristics (the wavelength, ripple height, ripple index, and symmetry coefficient) for 142 granule ripples by measuring their wavelength, ripple height, and ridge position. The ripple index (<em>Z</em>) equaled the ratio of the wavelength to the ripple height. The symmetry coefficient equaled the ratio of the horizontal windward slope length to the horizontal leeward slope length.</p> <p>Wavelength represents the horizontal distance between two crests, and the ripple height is the perpendicular height from the trough to the crest.</p> <p>We also excavated vertical profiles of five typical granule ripples with different sizes, and measured the stratification thickness for one ripple with a wavelength of 5 m, for a vertical profile whose depth was 60 cm. Based on the different grain size characteristics, we identified five strata and therefore obtained five sediment samples with different depths, and analyzed the vertical distribution of particle sizes in the sediment.</p> <p>We installed HOBO U30 field anemometers (Onset Computers, Bourne, MA, USA) at a height of 2 m and installed an eight-direction sand trap in the two areas in January 2011 to obtain the wind speed, wind direction, and the single-width sediment transport for a full year. The sensor type of wind speed and direction was S-WSET-A. The collection frequency for the wind speed and direction was 10 minutes, and the resolution of the wind speed and direction were 0.38 m/s and 1.4°, respectively. It is worth mentioning that Onset anemometers have a 5-degree window where they cannot measure wind direction, between 355 and 0 degrees. Unlike sand ripples, granule ripples are not particularly sensitive to wind direction, and these wind direction data only provide a regional main direction for the research, so a 5-degree window has little impact. The collection frequency of the sediment transport rate was 1 month, and the resolution was 10 g. The width and height of each collecting port of the sand trap were 2 cm and 1.2 m, respectively.</p> <p>2. Wind tunnel experiment to detect the gravel threshold velocity</p> <p>We performed a wind tunnel simulation experiment to test the impact threshold velocity of the gravels. We used the high-speed wind tunnel at the High Speed Railway Construction Technology National Engineering Laboratory of Central South University in Changsha, Hunan Province in the validation test. We used surface sediments from granule ripples collected at our study area, and screened these sediments through 12 sieves with a mesh size ranging from 1.6 to 31.5 mm. We used the sieved material to create 10 different test surfaces with an average particle size ranging from 1.8 to 25.8 mm, which we subsequently bombarded with saltating particles from an upwind source. The upwind supply rate for the impacting particles was 1.2 kg/min, and their particle size ranged from 0.8 to 1.0 mm, with an average size of 0.9 mm, in all tests. The impacting particles were also obtained from the surface sediments of the granule ripples and were obtained by screening. The gravel bed surface was 80 cm long (parallel to the long axis of the wind tunnel), 40 cm wide, and 3 cm deep with its upper surface level with the wind tunnel floor. To increase the roughness of the surface, we glued a layer of coarse sand with a mean particle size of 0.9 mm that was 4.2 m long by 40 cm wide to the floor of the wind tunnel immediately upwind of the gravel bed.</p> <p>We observed the particles with a high-speed camera in the wind tunnel to determine the type of motion (vibration, roll, obvious roll, saltation, obvious saltation). The wind velocities were measured using a pitot tube at 10 heights (6, 7.5, 13, 21, 41, 83, 123, 161, 220, and 242 mm above the gravel bed).</p> <p>3. Measurement of the granule ripple migration rate</p> <p>We chose a 25-m<sup>2</sup> area at the yardang site, and monitored the migration rate of some smaller typical granule ripples from 11 October 2011 to 15 October 2012 using six marking pins, at an east–west spacing of 5 m between pins. The average wavelength of these granule ripples was 1.6 m and the average height was 0.09 m. The mean grain size in the ridge was 2.08 mm, and the maximum grain size was 6.5 mm. We also chose 11 larger granule ripples (5 to 9 m in wavelength, 30 to 50 cm in height, granule size in the ridge of 6 to 10 mm) that were continuously distributed at the yardang site, and measured their migration rate over an 8-year period from 2011 to 2019 by using marking pins. Based on the migration rate, we applied the equation developed by Jerolmack et al. (2006) to calculate the sediment creep flux (<em>q</em><sub>c</sub>) at the ridge of the granule ripples:</p> <p><em>q</em><em><sub>c</sub></em> = (1-<em>p</em>) <em>ρ</em><em>cH </em>/ 2</p> <p>where<em> p</em> is the porosity of the ripple sediment (30%), ρ is the particle density (2650 kg m<sup>-3</sup>), <em>c</em> is the ripple migration rate (m/year), and <em>H</em> is the ripple height (m).</p>
FIGURE 18. Amblyseius eharai, Hypostome. A in Morphological changes and ontogenetic development of Amblyseius eharai Amitai & Swirski (Acari: Phytoseiidae)
FIGURE 18. Amblyseius eharai, Hypostome. A. Larva; B. Protonymph; C. Deutonymph; D. Female; E. Male; F. Spermatheca
Supplementary material 3 from: Vinasco AFM, Carrejo NSG (2016) Morphology and development rate of the immature stages of Glyphidops (Oncopsia) flavifrons (Bigot, 1886) (Diptera, Neriidae) under natural conditions. ZooKeys 603: 141-159. https://doi.org/10.3897/zookeys.603.7355
Egg hatching of Glyphidops (Oncopsia) flavifrons : Explanation note: Moments before the hatching, the larva is observed moving the body and the head, rubbing its mandibles against the inner wall of the egg and finally thrusting the corium. The larva emerges from the anterior part of the egg by using one of the two longitudinally lateral hatching lines.
Supplementary material 2 from: Vinasco AFM, Carrejo NSG (2016) Morphology and development rate of the immature stages of Glyphidops (Oncopsia) flavifrons (Bigot, 1886) (Diptera, Neriidae) under natural conditions. ZooKeys 603: 141-159. https://doi.org/10.3897/zookeys.603.7355
Mating and oviposition process of Glyphidops (Oncopsia) flavifrons : Explanation note: The body of each egg is buried in the substrate and the filament is spread over the surface. As the female began laying eggs (reaching packages up to 20 eggs, one egg at a time), it was observed that numerous filaments were emerging and radiating from the same point. Along this process, the male remains close to the female and the mating happen continuously between laid eggs.
Supplementary material 1 from: Vinasco AFM, Carrejo NSG (2016) Morphology and development rate of the immature stages of Glyphidops (Oncopsia) flavifrons (Bigot, 1886) (Diptera, Neriidae) under natural conditions. ZooKeys 603: 141-159. https://doi.org/10.3897/zookeys.603.7355
Cluster analysis of mandibular area and hypopharyngeal sclerite : Explanation note: Cluster analysis of mandibular area and hypopharyngeal sclerite length across time supports the concept of three larval instar (similarity indexes between 95% and 100%). (a) body length of the larva, (b) mandibular area and (c) length of hypopharyngeal sclerite. Each color represents a larval instar (Ln).
Fig. 8 in Early development of Monoplex pilearis and Monoplex parthenopeus (Gastropoda: Cymatiidae): biology and morphology
Fig. 8 PCA analysis of M. pilearis and M. parthenopeus outlines. As given by DiaOutline, PC 1 and PC 2 shown. M. pilearis data space is shown in green and M. parthenopeus data space in red
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Allen Brain Atlas
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