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Fig. 5 in Identification and functional characterization of two acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) genes from forage sorghum (Sorghum bicolor) embryo

Fig. 5. Overexpression of SbDGAT1 genes and its variants in yeast TAG mutant H1246. (A) Schematic representation of the N-terminal region of SbDGAT1 genes. Red arrow indicates the position of truncation in SbDGAT1-1. (B) TAG mutant H1246 strain transformed with SbDGAT1 full-length and truncated variants restores the TAG biosynthesis capability compared with control as visualized in TLC. The spots of triacylglycerol (TAG), diacylglycerol (DAG) and free fatty acids (FFA) are separated in TLC plate (C) Total TAG content of yeast H1246 cells expressed with full-length (SbDGAT1-1 and SbDGAT1-2) and truncated variants (SbDGAT1-1(39-515) and SbDGAT1-1(89-515)). Wild type yeast cells (BY4741) were used as positive control. (D) FA profile of TAG products isolated from yeast H1246 strain expressed with full-length and truncated variants of SbDGAT1 genes. Saturated FA abbreviations are as follows: 10:0, Capric acid; 12:0, Lauric acid; 14:0, Myristic acid; 15:0, Pentadecylic acid; 16:0, Palmitic acid; 18:0, Stearic acid. Mono-unsaturated FA abbreviations are as follows: 14:1, Myristoleic acid; 16:1, Palmitoleic acid; 18:1, Oleic acid. The error bars represent the SD of three biological replicates. Asterisks indicates significant differences according to student t-test results where * = p <0.05, ** = p <0.01. H1246 transformed with the empty pYES2 vector served as negative control and wild type yeast (BY4741) served as a positive control. FA, fatty acid; DW, dry weight. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 3 in Identification and functional characterization of two acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) genes from forage sorghum (Sorghum bicolor) embryo

Fig. 3. DISOPRED plot representing the intrinsically disordered regions in (A) SbDGAT1-1 (B) SbDGAT1-2 protein.

Fig. 6 in Identification and functional characterization of two acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) genes from forage sorghum (Sorghum bicolor) embryo

Fig. 6. LB observation of yeast H1246 transformants with SbDGAT1 variants using confocal microscopy. H1246 mutant strains (transformed with the empty pYES2 vector) and SbDGAT1 variants were observed under confocal microscope. Wild type yeast cells (BY4741) were used as positive control and H1246 as negative control. BODIPY493/503 dyes were used to visualize the yeast LBs.

Fig. 2 in Identification and functional characterization of two acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) genes from forage sorghum (Sorghum bicolor) embryo

Fig. 2. Sequence alignment of SbDGAT1-1 (XP_002439419) and SbDGAT1-2 (XP_021304830) deduced amino acid sequences with biochemically characterized AtDGAT1 (CAB45373). Putative N-terminal region is highlighted with blue colour. Conserved sequence domains are boxed, the acyl-CoA binding site (Jako et al., 2001; Ayme et al., 2015), active site (Jako et al., 2001), typical SnRK1 protein kinase binding motif (Zou et al., 1999; Xu et al., 2008), thiolase acyl-enzyme intermediate binding motif (Zou et al., 1999; Xu et al., 2008), FA binding protein signature (Guo et al., 2017), DAG-binding site (Guo et al., 2017) and ER retrieval motif (Aznar-Moreno et al., 2015). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 1. Phylogenetic relationship between SbDGAT1-1 and SbDGAT1-2 with other DGAT1 and DGAT2 in Identification and functional characterization of two acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) genes from forage sorghum (Sorghum bicolor) embryo

Fig. 1. Phylogenetic relationship between SbDGAT1-1 and SbDGAT1-2 with other DGAT1 and DGAT2 protein sequences. Phylogenetic tree was constructed using maximum likelihood method (Tamura et al., 2011; Hall, 2013) in Molecular Evolutionary Genetics Analysis (MEGA) 7.0 tool (Kumar et al., 2016). Bootstrap values are mentioned in the branches. GenBank accession numbers are indicated with species names of the DGAT proteins.

The Efficacy of Jobelyn (Sorghum Bicolor Extract)in the Treatment of Sickle Cell Anemia

ClinicalTrials.gov study NCT01703104. IPD Sharing: Not stated. Countries: 1. Publications: 12.

Quality of Life Study for Sickle Cell Patients Treated With Jobelyn (Sorghum Bicolor Extract)

ClinicalTrials.gov study NCT01704794. IPD Sharing: Not stated. Countries: 1. Publications: 12.

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Metabolic Availability of Lysine From Sorghum in Adult Men

ClinicalTrials.gov study NCT03411005. IPD Sharing: NO. Countries: 1. Publications: 1.

The Influence of Different Food Componente on Zinc Absorption in Young Adults Consuming Zinc Fortified Porridges Prepared From Maize, Beans and Sorghum: a Series of Six Similar Randomized, Single-blin

ClinicalTrials.gov study NCT01210794. IPD Sharing: Not stated. Countries: 1. Publications: 1.

Antipsychotic Effects of Sorghum Bicolor (JOBELYN) in the Treatment of Schizophrenia

ClinicalTrials.gov study NCT02240173. IPD Sharing: Not stated. Countries: 1. Publications: 8.