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325
datasets available to search
ShareScore release 0.9.0
Dataset results
325 results for “PURIFICATION”
Purification of pancreatic endocrine subsets from mouse islets
GEO Series GSE149488. Mus musculus. 9 samples. Type: Expression profiling by high throughput sequencing.
Bio NSD3 PWWP1 Expression and Purification Protocol
<p>Bio NSD3 PWWP1 Expression and Purification Protocol</p>
Recombinant human ACE2 protein production and purification
<p>Recombinant human ACE2 protein production and purification</p>
BIO NSD2 PWWP1 Expression and Purification Protocol
<p>Biotinylated NSD2 PWWP1 Expression and Purification Protocol</p>
20200520_FBXW7_349-707_NHis_Purification Protocol
<p>FBXW7_349-707_NHis_Expression and Purification Protocol</p>
Data from: Optimized application of the secreted Nano-luciferase reporter system using an affinity purification strategy
Secreted Nano-luciferase (secNluc) is a newly engineered secreted luciferase that possesses advantages of high structural stability, long half-life, and glow-type kinetics together with high light emission intensity, and thus would become one of the most valuable tools for bioluminescence assays. However, like other secreted luciferases, secNluc has to mix with the components in the conditioned medium surrounding test cells, or in the biological samples such as blood or urine after being secreted. These components may interfere with secNluc-catalyzed bioluminescence reactions and thus limit the application of the secNluc reporter system. In this study, we first examined the effects of three factors, pH, serum and residual reagents, on secNluc-catalyzed bioluminescence reactions, finding that these factors could interfere with bioluminescence reactions and result in background signal. To resolve these problems, we applied a simple affinity purification strategy in which secNluc was fused with a FLAG-tag, and anti-FLAG magnetic beads were used to catch and transfer the fusion protein to PBST, an optimal buffer for secNluc-catalyzed bioluminescence reactions that was identified in this study. The results indicated that this strategy could not only negate the interferences from serum or residual reagents and enhance the stability of light emission but also greatly increase signal intensity through enzyme enrichment. This strategy may contribute to biomedical studies that utilize secNluc and other secreted luciferases, especially those requiring superior sensitivity, low background noise and high reproducibility.
Blood Purification With Seraph 100 Microbind Affinity Blood Filter for Treatment of Severe COVID19 Observational Study
ClinicalTrials.gov study NCT04606498. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
Continuous Blood Purification for Regulation of Early Inflammatory Response In Severe Acute Pancreatitis
ClinicalTrials.gov study NCT01998334. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Safety and Effectiveness of Mesenchymal Stem Cells in Blood Purification for the Treatment of Liver Failure
ClinicalTrials.gov study NCT06904755. IPD Sharing: NO. Countries: 1. Publications: 0.
Clearance of Vasoactive Metabolites With Blood Purification
ClinicalTrials.gov study NCT06109142. IPD Sharing: NO. Countries: 1. Publications: 0.
Extracorporeal Blood Purification Using Oxiris for Septic Shock (EXPLORE Study)
ClinicalTrials.gov study NCT07334327. IPD Sharing: NO. Countries: 0. Publications: 0.
Blood Purification for the Treatment of Pathogen Associated Shock
ClinicalTrials.gov study NCT05011656. IPD Sharing: NO. Countries: 1. Publications: 0.
Blood Purification in Patients With Septic Shock
ClinicalTrials.gov study NCT04957316. IPD Sharing: NO. Countries: 1. Publications: 0.
Comparison of RCA and RACD in Extra-renal Purification by SLED
ClinicalTrials.gov study NCT04968587. IPD Sharing: NO. Countries: 1. Publications: 0.
Safety and Efficiency of Combined Extracorporeal Blood Purification in Neurosurgical ICU. Prospective RCT
ClinicalTrials.gov study NCT04152174. IPD Sharing: NO. Countries: 1. Publications: 0.
Blood Purification in Septic Children
ClinicalTrials.gov study NCT05595239. IPD Sharing: Not stated. Countries: 1. Publications: 0.
The Role of Blood Purification by Hemoadsorption as Adjunctive Treatment in Children With Septic Shock
ClinicalTrials.gov study NCT05401695. IPD Sharing: NO. Countries: 1. Publications: 0.
Impact of APP-assisted Patient Management on the Quality Control of Blood Purification Therapy
ClinicalTrials.gov study NCT07291154. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
Experimentation of Formaldehyde Purification by Plants in the Anatomy and Cytology Department
ClinicalTrials.gov study NCT07264504. IPD Sharing: NO. Countries: 1. Publications: 0.
Feasibility Trial of Extracorporeal Iron Purification in Patients With Myelodysplastic Syndrome or Myelofibrosis
ClinicalTrials.gov study NCT06781099. IPD Sharing: Not stated. Countries: 1. Publications: 0.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.