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302 results for “entomopathogen”
Figure 1 from: Hsu S-Y, Xu Y-C, Lin Y-C, Chuang W-Y, Lin S-R, Stadler M, Tangthirasunun N, Cheewangkoon R, AL-Shwaiman HA, Elgorban AM, Ariyawansa HA (2024) Hidden diversity of Pestalotiopsis and Neopestalotiopsis (Amphisphaeriales, Sporocadaceae) species allied with the stromata of entomopathogenic fungi in Taiwan. MycoKeys 101: 275-312. https://doi.org/10.3897/mycokeys.101.113090
Figure 1 The habitat of Pestalotiopsis and Neopestalotiopsis species, situated on the stromata of entomopathogenic fungi. Specimens AOphiocordyceps sp. NTUPPMH 18-160 BBeauveria sp. NTUPPMH 18-161 CTolypocladium sp. NTUPPMH 21-055 DOphiocordyceps sp. NTUPPMH 21-054 EOphiocordyceps sp. NTUPPMH 21-053 FOphiocordyceps sp. NTUPPMH 18-164 G, H the section of conidioma of Ophiocordyceps sp. showing the location of conidia of pestalotiopsis-like fungi (red arrow). Scale bars: 100 μm (G); 20 μm (H).
Figure 6 from: Hsu S-Y, Xu Y-C, Lin Y-C, Chuang W-Y, Lin S-R, Stadler M, Tangthirasunun N, Cheewangkoon R, AL-Shwaiman HA, Elgorban AM, Ariyawansa HA (2024) Hidden diversity of Pestalotiopsis and Neopestalotiopsis (Amphisphaeriales, Sporocadaceae) species allied with the stromata of entomopathogenic fungi in Taiwan. MycoKeys 101: 275-312. https://doi.org/10.3897/mycokeys.101.113090
Figure 6 The morphology of Pestalotiopsis manyueyuananiA the original habitat of conidia of Pestalotiopsis manyueyuanani; stroma of Ophiocordyceps sp. B top view (left) and bottom view (right) of the colony on potato dextrose agar (PDA) after incubation for seven days C formation of conidiomata on carnation leaf D, E conidiogenous cells and immature conidia F–K conidia L–N germinated conidia. Scale bars: 250 μm (C); 10 μm (D–K); 20 μm (L–N).
Figure 9 from: Hsu S-Y, Xu Y-C, Lin Y-C, Chuang W-Y, Lin S-R, Stadler M, Tangthirasunun N, Cheewangkoon R, AL-Shwaiman HA, Elgorban AM, Ariyawansa HA (2024) Hidden diversity of Pestalotiopsis and Neopestalotiopsis (Amphisphaeriales, Sporocadaceae) species allied with the stromata of entomopathogenic fungi in Taiwan. MycoKeys 101: 275-312. https://doi.org/10.3897/mycokeys.101.113090
Figure 9 Neopestalotiopsis haikouensis (NTUPPMCC 18-163 = CD04) A the original habitat of Neopestalotiopsis haikouensis; the stroma of Ophiocordyceps sp. B top view (left) and bottom view (right) of the colony on potato dextrose agar (PDA) after incubation for seven days C conidiomata on carnation leaf D, E conidiogenous cells and immature conidia F–I conidia. Scale bars: 250 μm (C); 20 μm (D–I).
Figure 11 from: Hsu S-Y, Xu Y-C, Lin Y-C, Chuang W-Y, Lin S-R, Stadler M, Tangthirasunun N, Cheewangkoon R, AL-Shwaiman HA, Elgorban AM, Ariyawansa HA (2024) Hidden diversity of Pestalotiopsis and Neopestalotiopsis (Amphisphaeriales, Sporocadaceae) species allied with the stromata of entomopathogenic fungi in Taiwan. MycoKeys 101: 275-312. https://doi.org/10.3897/mycokeys.101.113090
Figure 11 Temperature effect on mycelial growth according to the comparison of colonial growth (mm) of different species at each temperature, based on the mean values presented in Table S11. Colours represent different taxa: NTUPPMCC 18-160, Pestalotiopsis trachycarpicola; NTUPPMCC 18-161, Neopestalotiopsis sp.; NTUPPMCC 18-162, Pestalotiopsis hispanica; NTUPPMCC 18-163, Neopestalotiopsis haikouensis; NTUPPMCC 18-165, Pestalotiopsis manyueyuanani.; NTUPPMCC 18-166, Neopestalotiopsis camelliae-oleiferae; NTUPPMCC 21-054, Pestalotiopsis chamaeropis; NTUPPMCC 21-056, Pestalotiopsis formosana.
Figure 2 from: Fan Q, Yang T, Li H, Wang X-M, Liao H-F, Shen P-H, Yang Z-L, Zeng W-B, Wang Y-B (2024) Molecular phylogeny and morphology reveal two new entomopathogenic species of Ophiocordyceps (Ophiocordycipitaceae, Hypocreales) parasitic on termites from China. MycoKeys 103: 1-24. https://doi.org/10.3897/mycokeys.103.116153
Figure 2 Ophiocordyceps longistipesA, B stromata of fungus arising from termites C fertile part D perithecia E colony on PDA (obverse and reverse) F, G ascospores H, I asci J–L conidiogenous cells and conidia. Scale bars: 1 cm (A, B, E); 2 mm (C); 100 µm (D); 20 µm (F–L).
Figure 1 from: Fan Q, Yang T, Li H, Wang X-M, Liao H-F, Shen P-H, Yang Z-L, Zeng W-B, Wang Y-B (2024) Molecular phylogeny and morphology reveal two new entomopathogenic species of Ophiocordyceps (Ophiocordycipitaceae, Hypocreales) parasitic on termites from China. MycoKeys 103: 1-24. https://doi.org/10.3897/mycokeys.103.116153
Figure 1 Phylogenetic tree based on the combined dataset of nrSSU, nrLSU, tef-1α, rpb1, rpb2, and ITS showing the relationship of two new species on termites from China with other Ophiocordyceps species. Values at the nodes before and after the backslash are BI posterior probabilities (BI-PP greater than 0.60) and ML bootstrap proportions (ML-BP greater than 70%), respectively. New species described in this paper are shown in bold red.
Figure 3 from: Fan Q, Yang T, Li H, Wang X-M, Liao H-F, Shen P-H, Yang Z-L, Zeng W-B, Wang Y-B (2024) Molecular phylogeny and morphology reveal two new entomopathogenic species of Ophiocordyceps (Ophiocordycipitaceae, Hypocreales) parasitic on termites from China. MycoKeys 103: 1-24. https://doi.org/10.3897/mycokeys.103.116153
Figure 3 Ophiocordyceps globiperitheciataA stromata of fungus arising from termites B sterile tip and fertile part C fertile part D perithecia E–G asci H–J ascospores. Scale bars: 1 cm (A); 2 mm (B); 500 µm (C); 50 µm (D); 20 µm (E–J).
Data from: Physiological and transcriptional immune responses of a non-model arthropod to infection with different entomopathogenic groups
<p>Insect immune responses to multiple pathogen groups including viruses, bacteria, fungi, and entomopathogenic nematodes have traditionally been documented in model insects such as <em>Drosophila melanogaster</em>, or medically important insects such as <em>Aedes aegypti</em>. Despite their potential importance in understanding the efficacy of pathogens as biological control agents, these responses are infrequently studied in agriculturally important pests. Additionally, studies often neglect to investigate responses against different pathogen groups, and typically focus on only a single time point during infection. As such, a robust understanding of immune system responses over the time of infection is often lacking. This study was conducted to understand how 3<sup>rd</sup> instar larvae of the major insect pest <em>Helicoverpa zea</em> responded over time to infection by four different pathogenic groups: viruses, bacteria, fungi, and entomopathogenic nematodes. Physiological immune responses were assessed at 4-, 24-, and 48-hours post-infection by measuring hemolymph phenoloxidase concentrations, hemolymph prophenoloxidase concentrations, hemocyte counts, and encapsulation ability. Transcriptional immune responses were measured at 24-, 48-, and 72-hours post-infection by quantifying the expression of <em>PPO2</em>, <em>Argonaute-2</em>, <em>JNK</em>, <em>Dorsal</em>, and <em>Relish</em>. This gene set covers the major known immune pathways: phenoloxidase cascade, siRNA, JNK pathway, Toll pathway, and IMD pathway. Our results indicate <em>H. zea</em> has an extreme immune response to <em>Bacillus thuringiensis</em> bacteria, a mild response to <em>Helicoverpa armigera</em> nucleopolyhedrovirus, and no detectable response to either the fungus <em>Beauveria bassiana</em> or <em>Steinernema carpocapsae </em>nematodes.</p>
Figures 4-9 from: Keller S, Hülsewig T (2018) Amended description and new combination for Entomophthora nebriae Raunkiaer, (1893), a little known entomopathogenic fungus attacking the ground beetle Nebria brevicollis (Fabricius, 1792). Alpine Entomology 2: 1-5. https://doi.org/10.3897/alpento.2.22136
Figures 4-9 4. Rhizoid with dark and sparsely branched ending together with resting spores (LPAO). 5. Cystidium with numerous nuclei (LPAO). 6. Dense layer of conidiophores with developing primary conidia (LPAO). 7. Primary conidia with prominent central vacuole (LPCB). 8. Young resting spores with nuclei (LPAO). 9. Mature resting spores with thick walls and one to several vacuoles depending on the stage of maturation (LPAO).
Figures 1-3 from: Keller S, Hülsewig T (2018) Amended description and new combination for Entomophthora nebriae Raunkiaer, (1893), a little known entomopathogenic fungus attacking the ground beetle Nebria brevicollis (Fabricius, 1792). Alpine Entomology 2: 1-5. https://doi.org/10.3897/alpento.2.22136
Figures 1-3 1. Nebria brevicollis at an early stage of fungus sporulation. At this stage the beetle is still able to move it's legs and antennae (nat. length of the beetle about 13 mm). 2. Beetle with fully sporulating fungus showing the extremely swollen abdomen. The strong cystidia are clearly visible at the edge of the fungus mass. 3. At the end of the sporulating period the mycelium turns yellowish (Photos: T. Hülsewig).
Figure 6 in A comparative study on the virulence of entomopathogenic fungi against Trogoderma granarium (Everts) (Coleoptera: Dermestidae) in stored grains rice
Figure 6. Mortality (%) of T. granarium grubs s exposed to Beauveria bassiana at different concentrations. Different letters above the bars represent the significant difference at P=0.05.
Figure 3 from: Sharma L, Oliveira I, Torres L, Marques G (2018) Entomopathogenic fungi in Portuguese vineyards soils: suggesting a 'Galleria-Tenebrio-bait method' as bait-insects Galleria and Tenebrio significantly underestimate the respective recoveries of Metarhizium (robertsii) and Beauveria (bassiana). MycoKeys 38: 1-23. https://doi.org/10.3897/mycokeys.38.26970
Figure 3 Principal component analysis (PCA) and hierarchical clustering of the observations based on the fungal isolations. aPC1 vs. PC2. bPC1 vs. PC3. cPC2 vs. PC3. d PCA 3D plot e Hierarchical clustering dendrogram to access the ecological proximities of obtained fungi based on their respective isolation profiles. Software R 4.3.2 was used to obtain the PCA plots and the hierarchical clustering. There was no fungal isolation from hedgerows from the farm Granja when bait-insect T.molitor was used and hence, it could not be included in any of the analysis which relies on proportions, i.e. PCA plots, hierarchical clustering. To reduce any bias, the authors also discarded the soil samples (N=1) which yielded the fungal isolations, when G.mellonella was used, from the hedgerows of the farm Granja. The blue balls represent relatively more frequent EPF, i.e. Beauveriabassiana, Beauveriapseudobassiana, Clonostachysroseaf.rosea, Cordycepscicadae, Purpureocilliumlilacinum and Metarhiziumrobertsii. The red balls represent other fungi such as Cordyceps sp., Lecanicilliumaphanocladii, Lecanicilliumdimorphum, Metarhiziumguizhouense and Purpureocilliumlavendulum. Hierarchical clustering based dendrogram classified isolated EPF into two clusters, i.e. rarely occurring EPF (cluster 1) and relatively more frequent EPF (cluster 2). Abbreviations used are: Beauveriabassiana (B.b), Beauveriapseudobassiana (B.p), Cordycepscicadae (C.c), Cordyceps sp. (C.sp), Lecanicilliumaphanocladii (L.a), Lecanicilliumdimorphum (L.d), Metarhiziumguizhouense (M.g), Purpureocilliumlavendulum (P.la), Purpureocilliumlilacinum (P.l), Clonostachysroseaf.rosea (C.rr) and Metarhiziumrobertsii (M.r).
Figure 2 from: Sharma L, Oliveira I, Torres L, Marques G (2018) Entomopathogenic fungi in Portuguese vineyards soils: suggesting a 'Galleria-Tenebrio-bait method' as bait-insects Galleria and Tenebrio significantly underestimate the respective recoveries of Metarhizium (robertsii) and Beauveria (bassiana). MycoKeys 38: 1-23. https://doi.org/10.3897/mycokeys.38.26970
Figure 2 Effect of insect baiting and habitat-type on the isolation of the entomopathogenic fungi. a Occurrence (% of soil samples ± SE) of entomopathogenic fungi when different bait-insects were incorporated b Occurrence (% of soil samples ± SE) of entomopathogenic fungi when soils were collected from different habitat-types. Bars with asterisk (*) show significant isolations, i.e. (P<0.05).
Figure 1 from: Sharma L, Oliveira I, Torres L, Marques G (2018) Entomopathogenic fungi in Portuguese vineyards soils: suggesting a 'Galleria-Tenebrio-bait method' as bait-insects Galleria and Tenebrio significantly underestimate the respective recoveries of Metarhizium (robertsii) and Beauveria (bassiana). MycoKeys 38: 1-23. https://doi.org/10.3897/mycokeys.38.26970
Figure 1 Geographic coordinates and altitudes of the farms and details of the soil sampling strategy adopted. a Details of the six farms of the Douro Wine Region, Portugal, which were considered in this study b Details of the soil sampling strategy from vineyards and adjacent hedgerows.
Supplementary material 1 from: Sharma L, Oliveira I, Torres L, Marques G (2018) Entomopathogenic fungi in Portuguese vineyards soils: suggesting a 'Galleria-Tenebrio-bait method' as bait-insects Galleria and Tenebrio significantly underestimate the respective recoveries of Metarhizium (robertsii) and Beauveria (bassiana). MycoKeys 38: 1-23. https://doi.org/10.3897/mycokeys.38.26970
Supplementary tables :
Figure 1 from: Xiao Y-P, Hongsanan S , Hyde KD, Brooks S, Xie N, Long F-Y, Wen T-C (2019) Two new entomopathogenic species of Ophiocordyceps in Thailand. MycoKeys 47: 53-74. https://doi.org/10.3897/mycokeys.47.29898
Figure 1 - Phylogram of Ophiocordyceps globiceps and O. sporangifera generated from maximum likelihood (RAxML) analysis of ITS, SSU, LSU, RPB1 and TEF1α sequence data. Tolypocladium inflatum and T. ophioglossoides were used as outgroup taxon. Maximum likelihood bootstrap values greater than 75% and Bayesian posterior probabilities over 0.90 were indicated above the nodes. The new species are indicated in red.
Figure 4 from: Xiao Y-P, Hongsanan S , Hyde KD, Brooks S, Xie N, Long F-Y, Wen T-C (2019) Two new entomopathogenic species of Ophiocordyceps in Thailand. MycoKeys 47: 53-74. https://doi.org/10.3897/mycokeys.47.29898
Figure 4 - Ophiocordyceps sporangifera (culture) MFLUCC 18–0492. a Upper side of the culture b Reverse side of the culture c, d Synnemata growing on PDA medium e, g Synnemata f Mycelium h–j Phialides k Conidia l–n Conidia form mucilaginous spheres. Scale bars: 1 cm (a, b), 5000 µm (c), 1000 µm (d), 500 µm (e), 100 µm (f, g), 50 µm (h–j), 10 µm (k–n).
Figure 3 from: Xiao Y-P, Hongsanan S , Hyde KD, Brooks S, Xie N, Long F-Y, Wen T-C (2019) Two new entomopathogenic species of Ophiocordyceps in Thailand. MycoKeys 47: 53-74. https://doi.org/10.3897/mycokeys.47.29898
Figure 3 - Ophiocordyceps sporangifera (holotype MFLU 18–0658). a Habitat b Synnemata on host surface c Host d, e Synnemata f Fertile head of primary synnema g Sporangium h Secondary synnema i Sporangium j, k, q Part of secondary synnema l Phialides m Conidia bound by deliquescing mucilaginous material n–p Conidia. Scale bars: 1 cm (c, d), 1000 µm (e), 200 µm (f, h, q), 100 µm (g, i), 50 µm (j), 20 µm (k, l), 10 µm (m–p).
Figure 2 from: Xiao Y-P, Hongsanan S , Hyde KD, Brooks S, Xie N, Long F-Y, Wen T-C (2019) Two new entomopathogenic species of Ophiocordyceps in Thailand. MycoKeys 47: 53-74. https://doi.org/10.3897/mycokeys.47.29898
Figure 2 - Ophiocordyceps globiceps (holotype MFLU 18–0661). a Habitat b Ascostroma emerging from infected fly c Host d Fertile head of ascostroma e Vertical section of the stroma f Section of ascomata g Peridium h, i Asci k Apical cap of asci l, q Part of ascospore m, n Secondary ascospores o Upper side of the culture p Reverse side of the culture. Scale bars: 1000 µm (b–d), 500 µm (e, f), 100 µm (h, i), 20 µm (g), 10 µm (k, l), 5 µm (m, n, q), 5 cm (o, p).
Supplementary material 1 from: Bustamante DE, Oliva M, Leiva S, Mendoza JE, Bobadilla L, Angulo G, Calderon MS (2019) Phylogeny and species delimitations in the entomopathogenic genus Beauveria (Hypocreales, Ascomycota), including the description of B. peruviensis sp. nov. MycoKeys 58: 47-68. https://doi.org/10.3897/mycokeys.58.35764
: Data type: molecular data
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