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restrictedSep 2022View details →

dataset related to the article:"N-Acetylcysteine Regenerates In Vivo Mercaptoalbumin"

This record contains raw data related to the article: "N-Acetylcysteine Regenerates In Vivo Mercaptoalbumin" Abstract: Human serum albumin (HSA) represents the most abundant plasma protein, with relevant antioxidant activity due to the presence of the sulfhydryl group on cysteine at position 34 (Cys34), the latter being one of the major target sites for redox-dependent modifications leading to the formation of mixed disulfide linkages with low molecular weight thiols. Thiolated forms of HSA (Thio-HSA) may be useful as markers of an unbalanced redox state and as a potential therapeutic target. Indeed, we have previously reported that albumin Cys34 can be regenerated in vitro by N-Acetylcysteine (NAC) through a thiol-disulfide breaking mechanism, with a full recovery of the HSA antioxidant and antiplatelet activities. With this pilot study, we aimed to assess the ability of NAC to regenerate native mercaptoalbumin (HSA-SH) and the plasma antioxidant capacity in subjects with redox unbalance, after oral and intravenous administration. A placebo-controlled crossover study, single-blinded, was performed on six hypertensive subjects, randomized into two groups, on a one-to-one basis with NAC (600 mg/die, os) or a placebo. Albumin isoforms, HSA-SH, Thio-HSA, and glutathione levels were evaluated by means of mass spectrometry. The plasma antioxidant activity was assessed by a fluorimetric assay. NAC, orally administered, significantly decreased the Thio-HSA levels in comparison with the pre-treatment conditions (T0), reaching the maximal effect after 60 min (−24.7 ± 8%). The Thio-HSA reduction was accompanied by a concomitant increase in the native HSA-SH levels (+6.4 ± 2%). After intravenous administration of NAC, a significant decrease of the Thio-HSA with respect to the pre-treatment conditions (T0) was observed, with a maximal effect after 30 min (−68.9 ± 10.6% vs. T0) and remaining significant even after 6 h. Conversely, no effect on the albumin isoforms was detected with either the orally or the intravenously administered placebo treatments. Furthermore, the total antioxidant activity of the plasma significantly increased after NAC infusion with respect to the placebo (p = 0.0089). Interestingly, we did not observe any difference in terms of total GSH corrected for hemoglobin, ruling out any effect of NAC on the intracellular glutathione and supporting its role as a disulfide-breaking agent. This case study confirms the in vitro experiments and demonstrates for the first time that NAC is able to regenerate mercaptoalbumin in vivo, allowing us to hypothesize that the recovery of Cys34 content can modulate in vivo oxidative stress and, hopefully, have an effect in oxidative-based diseases.

restrictedDec 2022View details →

Dataset related to article "Transversal hepatectomies: Classification and intention-to-treat validation of new parenchyma-sparing procedures for deep-located hepatic tumors"

This record contains raw data related to article “Transversal hepatectomies: Classification and intention-to-treat validation of new parenchyma-sparing procedures for deep-located hepatic tumors"   Background: Deep-located liver tumors involving hepatic veins at the caval confluence or main Glisso- nean pedicles generally require a major hepatectomy. An intraoperative ultrasound guidance policy opened a possibility to opt for parenchyma-sparing procedures as alternatives to major hepatectomy, called transversal hepatectomies. We ought to standardize the procedure and analyze the surgical outcome, oncological suitability, and salvageability. Methods: This is a retrospective cohort study. All consecutive patients undergoing hepatectomies for liver tumors between January 2005 and August 2020 were reviewed. Transversal hepatectomies were classified as follows: upper transversal hepatectomy: resection of the posterosuperior segments along with at least 1 hepatic vein and preservation of the anteroinferior ones; roller coaster hepatectomy: transversal hepatectomy with tumor vessel detachment from at least 2 hepatic veins; and lower transversal hepatectomy: amputation of the distal portion of at least 1 hepatic vein with tumor vessel detachment from first/second-order Glissonean pedicles. Morbidity, mortality, local recurrences, and salvageability in cases of relapse were considered. Results: A total of 61 transversal hepatectomies were performed: 40 (66%) upper transversal hepatec- tomies, 19 (31%) roller coaster hepatectomies, and 2 (3%) lower transversal hepatectomies. The median preserved liver volume was 67% (range 41e86). Mortality was 0, and major morbidity was 6%. Local recurrence occurred in 7 (11%) patients. Ten out of 34 (29%) patients with liver-only recurrence received redo surgery. Conclusion: Transversal hepatectomies offer a new parenchyma-sparing perspective for the manage- ment of complex tumor presentation, which would otherwise demand major tissue removal or even unresectability. Safety, adequate local control, and salvageability are further pillars of this approach herein systematized.

restrictedJun 2021View details →

Dataset related to article "In-depth genetic and molecular characterization of Diaphanous Related Formin 2 (DIAPH2) and its role in the inner ear"

This record contains data (Table S1 to S4, Figure S1 to S12, File S1) related to article “In-depth genetic and molecular characterization of Diaphanous Related Formin 2 (DIAPH2) and its role in the inner ear” .  Specifically, the record contains the following supplementary information to the article: Table S1. Coding sequence coverage of known autosomal recessive and X-linked NSHL-causing genes in exome data. Table S2. Prioritized variants shared between affected siblings III1 and III3. Table S3. CNVs shared between affected siblings III1 and III3. Table S4. Primers used for genetic screening of candidate genes/variants. Figure S1. Analysis of auditory brainstem evoked potentials in proband III3. Figure S2. Expression of mouse Diaph2 mRNA in P4 organ of Corti by RT-PCR.  Figure S3. Diaph2 expression in E14.5 and E16.5 wild-type mouse cochlea. Figure S4. Evaluation of Diaph2 expression in whole-mount mouse cochleas. Figure S5. Diaph2 expression in P7 and P14 wild-type mouse cochlea. Figure S6. In-silico analysis of the impact of c.868A>G variant on DIAPH2 pre-mRNA splicing. Figure S7. In-vitro analysis of the impact of c.868A>G variant on DIAPH2 pre-mRNA splicing. Figure S8. In-vitro characterization of the effect of the c.868A>G variation on splicing using a DIAPH2minigene spanning exons 6 to 9. Figure S9. Analysis of DIAPH2 exon 8 splicing in blood from NSHL3 family subjects. Figure S10. In-vivo analysis of Diaph2 exon 8 splicing in the mouse cochlea. Figure S11. Pathogenicity prediction of the p.I290V missense variant with 8 commonly used software. Figure S12. DIAPH2 immunolocalization studies in basal conditions. File S1. Supplementary Methods

Dataset related to article: "Time-dependent effect of GLP-1 receptor agonists on cardiovascular benefits: a real-world study"

This record contains data related to article "Time-dependent effect of GLP-1 receptor agonists on cardiovascular benefits: a real-world study" Background: Glucagon-like peptide-1 receptor agonists (GLP-1 RAs) have shown cardiovascular benefits in cardiovascular outcome trials in type 2 diabetes mellitus. However, the most convincing evidence was obtained in subjects with established cardiovascular (CV) disease. We analyzed the determinants of GLP-1 RA-mediated CV protection in a real-world population of persons with type 2 diabetes with and without a history of CV events with long-term follow-up.Methods: Retrospective cohort study of 550 individuals with type 2 diabetes (395 in primary CV prevention, 155 in secondary CV prevention), followed at a single center after the first prescription of a GLP-1 RA between 2009 and 2019. CV and metabolic outcomes were assessed.Results: Median duration of follow-up was 5.0 years (0.25-10.8) in primary prevention and 3.6 years (0-10.3) in secondary prevention, with a median duration of treatment of 3.2 years (0-10.8) and 2.5 years (0-10.3) respectively. In the multivariable Cox regression model considering GLP-1 RA treatment as a time-dependent covariate, in the primary prevention group, changes in BMI and glycated hemoglobin did not have an impact on MACE risk, while age at the time of GLP-1 initiation (HR 1.08, 95% CI 1.03-1.14, p = 0.001) and GLP-1 RA cessation by time (HR 3.40, 95% CI 1.82-6.32, p < 0.001) increased the risk of MACE. Regarding the secondary prevention group, only GLP-1 RA cessation by time (HR 2.71, 95% CI 1.46-5.01, p = 0.002) increased the risk of MACE. With respect to those who withdrew treatment, subjects who continued the GLP-1 RA had significantly greater weight loss and lower glycated hemoglobin levels during follow-up.Conclusions: In this real-world type 2 diabetes population, discontinuation of GLP-1 RA treatment was associated to a higher risk of major cardiovascular events, in both subjects with and without a history of CV events.

Dataset for the article "Cerebrospinal fluid/serum albumin quotient (Q-Alb) is not increased in Alzheimer's disease compared to neurological disease controls: a retrospective study on 276 patients"

This is the anonymized dataset for the article entitled "Cerebrospinal fluid/serum albumin quotient (Q-Alb) is not increased in Alzheimer's disease compared to neurological disease controls: a retrospective study on 276 patients".

Dataset for the article "Progressive motor neuron syndromes with single CNS lesions and CSF oligoclonal bands: never forget solitary sclerosis!"

This is the dataset for the article "Progressive motor neuron syndromes with single CNS lesions and CSF oligoclonal bands: never forget solitary sclerosis!"

Dataset for the article "Serum neurofilament light chain levels in Covid-19 patients without major neurological manifestations"

This is the dataset for the article "Serum neurofilament light chain levels in Covid-19 patients without major neurological manifestations".

Dataset pertaining to the article "Serum levels of glial fibrillary acidic protein in patients with amyotrophic lateral sclerosis"

This is the dataset pertaining to the article "Serum levels of glial fibrillary acidic protein in patients with amyotrophic lateral sclerosis".

"Dataset related to article "Long-term sequential digital dermoscopy of low-risk patients may not improve early diagnosis of melanoma compared to pe-riodical handheld dermoscopy"

This record contains data related to article "Long-term sequential digital dermoscopy of low-risk patients may not improve early diagnosis of melanoma compared to pe-riodical handheld dermoscopy"

data set related to article Callous-Unemotional Traits and Intelligence in Children with Externalizing Behavioral Problems

This record contains raw data related to article Callous-Unemotional Traits and Intelligence in Children with Externalizing Behavioral Problems.  Per la richiesta di accesso al file contattare il Dottore Pietro Muratori all'indirizzo email: <a href="mailto:pietro.muratori@fsm.unipi.it">pietro.muratori@fsm.unipi.it</a>

data set related to article Parenting practices and dysregulation profile in a sample of Italian children

This record contains raw data related to article Parenting practices and dysregulation profile in a sample of Italian children Per la richiesta di accesso al file contattare il Dr. Pietro Muratori all'indirizzo email pietro.muratori@fsm.unipi.it

dat scan IMAGES RELATED TO ARTICLE "Cerebrospinal fluid neuropathological biomarkers in beta-propeller protein-associated neurodegeneration, with complicated parkinsonian phenotype."

DAT SCAN IMAGES

Dataset related to article"THE SPTLC1 P.S331 MUTATION BRIDGES SENSORY NEUROPATHY AND MOTOR NEURON DISEASE AND HAS IMPLICATIONS FOR TREATMENT"

cmt gene panel data of SPTLC1 case

data set related to article Early Sensory Profile in Autism Spectrum Disorders Predicts Emotional and Behavioral Issues

This record contains raw data related to article Early Sensory Profile in Autism Spectrum Disorders Predicts Emotional and Behavioral Issues Per la richiesta di accesso al file contattare il Dr. Antonio Narzisi all'indirizzo antonio.narzisi@fsm.unipi.it

data set related to article Sensory Profiles in School-Aged Children with Autism Spectrum Disorder: A Descriptive Study Using the Sensory Processing Measure-2 (SPM-2)

This record contains raw data related to article Sensory Profiles in School-Aged Children with Autism Spectrum Disorder: A Descriptive Study Using the Sensory Processing Measure-2 (SPM-2) Per la richiesta di accesso al file contattare il Dr. Antonio Narzisi all'indirizzo email antonio.narzisi@fsm.unipi.it

DATASET RELATE TO ARTICLE "The Role of Bypass Surgery for the Management of Complex Intracranial Aneurysms in the Anterior Circulation in the Flow-Diverter Era: A Single-Center Series"

Datasheet with anonymized clinical data of patients involved in the study at title

data set related to article Lung ultrasound in young children with neurological impairment: A proposed integrative clinical tool for deaeration-detection related to feeding

This record contains raw data related to article Lung ultrasound in young children with neurological impairment: A proposed integrative clinical tool for deaeration-detection related to feeding Per la richiesta di accesso al file contattare la Dr.ssa Simona Fiori all'indirizzo email simona.fiori@fsm.unipi.it

data set related to article Reading Behaviors through Patterns of Finger-Tracking in Italian Children with Autism Spectrum Disorder

This record contains raw data related to article Reading Behaviors through Patterns of Finger-Tracking in Italian Children with Autism Spectrum Disorder Per la richiesta di accesso al file contattare il Dr. Antonio Narzisi all'indirizzo email antonio.narzisi@fsm.unipi.it

Dataset related to article: "PPAR GAMMA AGONIST LERIGLITAZONE RECOVERS ALTERATIONS DUE TO PANK2-DEFICIENCY IN HIPS-DERIVED ASTROCYTES"

The database contains the microxygraphy data included in the article at title

Dataset related to article: "GRASPING THE SEMANTIC OF ACTIONS: A COMBINED BEHAVIORAL AND MEG STUDY"

virtual channel data