Find research datasets worth reusing

FIGURE 3 A in Systematic position and composition of Merodon nigritarsis and M. avidus groups (Diptera, Syrphidae) with a description of four new hoverfLies species

FIGURE 3 A) Merodon aberrans, male; body, B) Merodon aberrans, male; hypandrium, C) Merodon aurifer, male; body, D) Merodon clavipes, male; body. Scale: A), C–D) 4 mm, B) 0.2 mm. l–lingula.

FIGURE 8 in Systematic position and composition of Merodon nigritarsis and M. avidus groups (Diptera, Syrphidae) with a description of four new hoverfLies species

FIGURE 8 Male of Merodon serrulatus. A) and B) surstyle lobe (basolateral protrusion on the posterior surstyle lobe marked with arrow), C) body. Scale: A–B) 0.2 mm, C) 2 mm.

FIGURE 6 Male genitalia. Lateral view, A in Systematic position and composition of Merodon nigritarsis and M. avidus groups (Diptera, Syrphidae) with a description of four new hoverfLies species

FIGURE 6 Male genitalia. Lateral view, A) Merodon erivanicus, surstyle lobe B) Merodon erivanicus, epandrium, C) Merodon italicus, epandrium. pl–posterior surstyle lobe.

FIGURE 5 A in Systematic position and composition of Merodon nigritarsis and M. avidus groups (Diptera, Syrphidae) with a description of four new hoverfLies species

FIGURE 5 A) Merodon ottomanus, male; body, B) Merodon ottomanus, male; metabasotarsomere, C) Merodon ottomanus, male; basoflagellomere, (D) Merodon italicus, male; basoflagellomere. Scale: A) 1 mm, B) 0.5 mm, D) 0.5 mm, C) 0.2 mm.

FIGURE 7 in Systematic position and composition of Merodon nigritarsis and M. avidus groups (Diptera, Syrphidae) with a description of four new hoverfLies species

FIGURE 7 Male of Merodon pruni. A) basoflagellomere, B) metatrochanter and metafemur (distinct thorn on metatrochanter marked with arrow), C) body. Scale: 1 mm.

FIGURE 12 Male genitalia. Surstyle lobe. Ventral view, A in Systematic position and composition of Merodon nigritarsis and M. avidus groups (Diptera, Syrphidae) with a description of four new hoverfLies species

FIGURE 12 Male genitalia. Surstyle lobe. Ventral view, A) Merodon nitidifrons, B) Merodon alagoezicus, C) Merodon satdagensis (basal extension marked with arrow), D) Merodon schachti, E) Merodon hakkariensis, F) Merodon lucasi (lamellar structure marked with arrow). Scale: 0.2 mm. al–anterior lobe of surstylus, pl–posterior lobe of surstylus.

FIGURE 15A AND 15B in Systematic position and composition of Merodon nigritarsis and M. avidus groups (Diptera, Syrphidae) with a description of four new hoverfLies species

FIGURE 15A AND 15B Strict consensus tree based on 896 equally parsimonious trees from analysis of combined 3'- and 5'- fragments of the COI gene. Length 2169 steps, Consistency index (CI) 32, Retention index (RI) 71. Filled circles represent non-homoplasious changes and open circles are homoplasious changes. Bootstrap support values are depicted near nodes (≥50). Numbers and letters after the species name referred to the DNA labcode IDs.

FIGURE 11 in Systematic position and composition of Merodon nigritarsis and M. avidus groups (Diptera, Syrphidae) with a description of four new hoverfLies species

FIGURE 11 Abdomen of male. Dorsal view, A) Merodon rutitarsis sp. n., B) Merodon quadraticus, C) Merodon toscanus. Scale: 1 mm.

FIGURE 20 in Systematic position and composition of Merodon nigritarsis and M. avidus groups (Diptera, Syrphidae) with a description of four new hoverfLies species

FIGURE 20 Metaleg of male (without metatarsi). Lateral view, A) Merodon longisetus sp. n., B) Merodon obstipus sp. n., C) Merodon cohurnus sp. n., D) Merodon rutitarsis sp. n. Scale: 1 mm.

FIGURE 18 Antenna. Lateral view, A in Systematic position and composition of Merodon nigritarsis and M. avidus groups (Diptera, Syrphidae) with a description of four new hoverfLies species

FIGURE 18 Antenna. Lateral view, A) Merodon longisetus sp. n., male, B) Merodon longisetus sp. n., female, C) Merodon obstipus sp. n., male, D) Merodon obstipus sp. n., female, E) Merodon cohurnus sp. n., male. Scale: 0.5 mm. gl–basoflagellomere.

FIGURE 14 in Systematic position and composition of Merodon nigritarsis and M. avidus groups (Diptera, Syrphidae) with a description of four new hoverfLies species

FIGURE 14 Tarsomeres of male. A) Merodon avidus, protarsus, dorsal view, B) Merodon avidus, protarsus, ventral view, C) Merodon avidus, metatarsus, dorsal view, D) Merodon avidus, metatarsus, ventral view, E) Merodon nigritarsis, protarsus, dorsal view, F) Merodon nigritarsis, protarsus, ventral view, G) Merodon nigritarsis, metatarsus, dorsal view, H) Merodon nigritarsis, metatarsus, ventral view, I) Merodon femoratus, protarsus, dorsal view, J) Merodon femoratus, metatarsus, dorsal view, K) Merodon rutitarsis sp. n., metatarsus, dorsal view. Scale: 0.5 mm.

FIGURE 27 in Systematic position and composition of Merodon nigritarsis and M. avidus groups (Diptera, Syrphidae) with a description of four new hoverfLies species

FIGURE 27 Male genitalia of Merodon obstipus sp. n. A) epandrium, lateral view, B) surstyle lobe, ventral view, C) hypandrium, lateral view, D) anterior end of male genitalia, ventral view. Scale: 0.5 mm. al–anterior lobe of surstylus, c–circus, l–lingual, pl– posterior lobe of surstylus.

Fig. 4 in Morphology and Phylogeny of Four Marine Scuticociliates (Protista, Ciliophora), with Descriptions of Two New Species: Pleuronema elegans spec. nov. and Uronema orientalis spec. nov.

Fig. 4. Uronema orientalis spec. nov. in vivo (A, E–J), after protargol (B–D, K) and silver nitrate staining (L). A, E – ventral views of a typical cell; B, C – ventral (B) and dorsal (C) views of the same specimen, showing infraciliature and nuclear apparatus; D, K – detailed infraciliature of buccal area, arrowhead in (K) shows the gap between the anterior part and posterior parts of membranelle 1; F, G – ventral views, to show different body shapes; H – posterior region of cell, arrow points to caudal cilia, arrowheads show somatic cilia; I – ventral view, arrow refers to blue irregularly-shaped crystal, arrowhead indicates dumbbell-shaped crystal; J – ventral view, arrow shows contractile vacuole, arrowhead marks buccal field; L – ventral view, arrow shows contractile vacuole pore. M1, 2, 3 – membranelles 1, 2 and 3; M1a – the anterior part of membranelle 1; M1b – the posterior part of membranelle 1; Ma – macronucleus; PM – paroral membrane; Sc – scutica. Scale bars: A, B, C = 50 μm; E–G, J, K = 80 μm.

Fig. 3 in Morphology and Phylogeny of Four Marine Scuticociliates (Protista, Ciliophora), with Descriptions of Two New Species: Pleuronema elegans spec. nov. and Uronema orientalis spec. nov.

Fig. 3. Pleuronema grolierei Wang et al., 2008 (A–H) and P. setigerum Calkins, 1902 (I–M) in vivo (A–D, I–K) and after protargol (E–H, L, M). A, I – ventral views of typical individuals, arrow in (A) shows contractile vacuole, arrowheads in (I) mark paroral membrane; B – ventral view, arrowheads mark oral cilia; C, K – ventral views, arrowheads point to caudal cilia; D – ventral view, arrow marks ingested algae, arrowhead shows irregularly-shaped crystals; E – anterior region, arrow shows the anterior part of membranelle 2; F – detailed structure of the buccal area; G – posterior region, arrowhead points to membranelle 3; H – macronucleus; J – ventral view, arrowheads show irregularly-shaped crystals; L – posterior region, arrowhead indicates the ring-like posterior end of M2a; M – detailed structure of the buccal area, arrow marks membranelle 3, arrowheads show preoral kineties. M1 – membranelle 1; M2a – the anterior part of membranelle 2; M2b – the posterior part of membranelle 2; Ma – macronucleus. Scale bars: A, I = 20 μm, M = 5 μm.

Fig. 1 in Morphology and Phylogeny of Four Marine Scuticociliates (Protista, Ciliophora), with Descriptions of Two New Species: Pleuronema elegans spec. nov. and Uronema orientalis spec. nov.

Fig. 1. Map and photographs of biotopes (A–D) in which the samples were collected. A – no. 1 swimming beach, Qingdao (36°06′N; 120°32′E); B – beach near Sculpture Garden, Qingdao (36°4′N; 120°29′E); C – coastal area of no. 1 swimming beach, Qingdao (36°06′N; 120°32′E), the site that is further into the sea than that of (A); D – a mangrove wetland in Shenzhen, Guangdong Province (22°30′N; 114°37′E).

Fig. 5 in Morphology and Phylogeny of Four Marine Scuticociliates (Protista, Ciliophora), with Descriptions of Two New Species: Pleuronema elegans spec. nov. and Uronema orientalis spec. nov.

Fig. 5. Phylogenetic tree inferred from the small subunit ribosomal RNA (SSU rRNA) gene sequences, showing the positions of Pleuronema elegans spec. nov., P. grolierei, P. puytoraci, and Uronema orientalis spec. nov. (in bold). Numbers at nodes represent the bootstrap values of maximum likelihood (ML) out of 1,000 replicates and the posterior probability of Bayesian analysis (BI). The scale bar corresponds to five substitutions per 100 nucleotide positions.

FIGURE 10 in Systematic position and composition of Merodon nigritarsis and M. avidus groups (Diptera, Syrphidae) with a description of four new hoverfLies species

FIGURE 10 Abdomen of male. Dorsal view, A) Merodon cohurnus sp. n., B) Merodon nigritarsis. Scale: 1 mm.

opencc-by-4.0Sep 2023View details →

Data from: Automatic patient-level recognition of four Plasmodium species on thin blood smear by a Real Time Detector Transformer (RT-DETR) object detection algorithm: a proof-of-concept and evaluation

Automatic patient-level recognition of four Plasmodium species on thin blood smear by a Real Time Dectector Transformer (RT-DETR) object detection algorithm: a proof-of-concept and evaluation Emilie Guemas, Baptiste Routier, Théo Ghelfenstein-Ferreira, Camille Cordier, Sophie Hartuis, Bénédicte Marion, Sébastien Bertout, Emmanuelle Varlet-Marie, Damien Costa, Grégoire Pasquier Abstract: Malaria remains a global health problem with 247 million cases and 619,000 deaths in 2021. Diagnostic of Plasmodium species is important for administering the appropriate treatment. The gold-standard diagnosis from accurate species identification remains the thin blood smear. Nevertheless, this method is time-consuming and requires highly skilled and trained microscopists. To overcome these issues, new diagnostic tools based on deep learning are emerging. This study aimed to evaluate the performances of a RT-DETR (Real-Time Detection Transformer)object detection algorithm to discriminate Plasmodium species on thin blood smears images. The algorithm was trained and validated on a dataset consisting in 24,720 images from 475 thin blood smears corresponding to 2,002,597 labels. Performances were calculated with a test dataset of 4,508 images from 170 smears corresponding to 358,825labels coming from six French university hospital. At the patient level, the RT-DETR algorithm exhibited an overall accuracy of 79.4% (135/170) with a recall of 74% (40/54) and 81.9% (95/116) for negative and positive smears, respectively. Among Plasmodium positive smears, the global sensitivity was 82.7% (91/110) with a sensitivity of 90% (38/42), 81.8% (18/22) and 76.1% (35/46) for P. falciparum, P. malariae and P. ovale/vivax, respectively. The YOLOv5 model achieved a World Health Organization (WHO) competence level 2 for species identification. Besides, the RT-DETR algorithm may be run in real-time on low-cost devices such as a smartphone and could be suitable for deployment in low-resource setting areas where microscopy experts are lacking. Data collection: The training and validation dataset included 24,720 pictures taken from 475 manually May Grunwald-Giemsa (MGG)-stained thin blood smears from the Montpellier University Hospital collection and for a smaller part from the Toulouse University Hospital collection. In Montpellier, the pictures were taken with a Flexcam C1 microscope camera (Leica) attached to a Leica DM 2000 microscope and Leica DF450C microscope camera adapted with a Leica DM2500 microscope at X1000 magnification. Labelling of pictures was performed manually, and then automatically with manual correction with a Computer Visual Annotation Tools (CVAT) free software. Nine categories of labels were used: white blood cells (n=3,338), red blood cells (n=1,887,781), platelets (n=48,520), Trypanosoma brucei (n=2,773), and red blood cells infected by P. falciparum (n=43,545), P. ovale (n=4,651), P. vivax (n=4,115), P. malariae (n=2,849) and Babesia divergens (n=5,142). The test dataset included 4,508 pictures taken from 170 thin blood smears from the same number of patients from the Parasitology laboratories of University Hospitals of Montpellier, Toulouse, Rouen, Lille, Nantes and Saint-Louis in Paris (Table 1). Among these 170 patients, 54 were not infected, including two patients with Howell-Jolly bodies, and 116 were infected with hematozoa. For each patient, between 20 and 30 photos were taken from one thin blood smear with at least one hematozoan parasite per picture for infected patients. Accurate species diagnostic was made by a senior parasitologist, and for recent smears, it was confirmed by specific PCR, either performed locally (Toulouse) or at the Malaria French National Reference Center (Montpellier, Saint Louis, Rouen, Lille, Nantes).

Fig. 2 in Morphology and Phylogeny of Four Marine Scuticociliates (Protista, Ciliophora), with Descriptions of Two New Species: Pleuronema elegans spec. nov. and Uronema orientalis spec. nov.

Fig. 2. Pleuronema elegans spec. nov. in vivo (A, B, D, H–L), after protargol (E–G, M–Q) and silver nitrate staining (C). A, H – ventral view of a representative individual; B – detailed view of cortex to demonstrate arrangement of extrusomes; C – part of silverline system; D – swimming trace; E, F – ventral (E) and dorsal (F) views of the same specimen, showing infraciliature and nuclear apparatus; G – detailed structure of the buccal area, arrow shows the straight posterior end of M2a; I – ventral view, arrow marks paroral membrane and arrowhead shows ingested algae; J – ventral view, arrow shows membranelle 1, arrowheads mark the anterior part of membranelle 2; K – ventral view, arrowheads point to caudal cilia; L – ventral view, arrowheads mark somatic cilia; M – infraciliature of ventral side, arrow indicates membranelle 1, arrowhead refers to paired basal bodies; N – posterior region, arrow shows V-shaped posterior part of membranelle 2, arrowheads point to preoral kineties, double-arrowhead indicates membranelle 3; O – anterior region, arrowhead shows the anterior part of membranelle 2; P – macronucleus; Q – detailed view of membranelle 3 (arrowhead). M1, 3 – membranelles 1 and 3; M2a – the anterior part of membranelle 2; M2b – the posterior part of membranelle 2; Ma – macronucleus; PK – preoral kinety; PM – paroral membrane. Scale bars: A, E, F, H, I = 50 μm, J = 20 μm.