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opencc-by-4.0Jun 2020View details →

Figure 1 in Flufenacet activity is affected by GST inhibitors in blackgrass (Alopecurus myosuroides) populations with reduced flufenacet sensitivity and higher expression levels of GSTs

Figure 1. Dose–response analysis of the fresh weight of four Alopecurus myosuroides populations treated with different dose rates of flufenacet estimated using a threeparameter log-logistic model (see Equation 1).

opencc-zeroJun 2024View details →

Dataset: American Express Company (AXP) Stock Performance

This dataset provides historical stock market performance data for specific companies. It enables users to analyze and understand the past trends and fluctuations in stock prices over time. This information can be utilized for various purposes such as investment analysis, financial research, and market trend forecasting.

Figure 2 in Expression and immunolocalisation of TpFABP as a candidate antigen for the serodiagnosis of rabbit Taenia pisiformis cysticercosis

Figure 2. Expression of rTpFABP and identification by rabbit antisera in western blotting. Lane (1) molecular weight markers; (2) purified rTpFABP protein; (3) rTpFABP protein reacted with negative rabbit serum (1:100 v/v dilutions) by western blotting analysis; (4) rTpFABP protein reacted with rabbit antisera (1:100 v/v dilutions) by western blotting analysis. Molecular masses (kDa) are indicated on the left.

Figure 1 in Expression and immunolocalisation of TpFABP as a candidate antigen for the serodiagnosis of rabbit Taenia pisiformis cysticercosis

Figure 1. Structural analysis of TpFABP. Alignment of the amino acid residue sequences of Taenia pisiformis FABP with T. solium and Echinococcus granulosus in primary structures. The secondary structure of the TpFABP amino acid residue sequence was predicted and is shown at the top of the alignment. The light-grey shading indicates the identical amino acid sequences, and locations of linear B-cell epitopes are marked with open boxes. TpFABP1, GU205472; TsFABP1, HQ259679; TsFABP2, AFS64570; EgFABP1, 1O8V_A; EgFABP2, AAK12095; H-FABP, NP_004093.

Figure 4 in Expression and immunolocalisation of TpFABP as a candidate antigen for the serodiagnosis of rabbit Taenia pisiformis cysticercosis

Figure 4. Dot-ELISA of naturally infected rabbit experimental sera with rTpFABP. The tan-yellow tint shows the positive reaction: A, negative control sera; B, positive antisera; C, sera at 0 day postinfection; D, sera at 7 days post-infection; E, sera at 14 days post-infection; F, sera at 21 days post-infection; G, sera at 28 days post-infection; H, sera at 35 days post-infection; I, sera at 42 days post-infection; J, sera at 49 days post-infection.

Figure 3 in Expression and immunolocalisation of TpFABP as a candidate antigen for the serodiagnosis of rabbit Taenia pisiformis cysticercosis

Figure 3. Immunolocalisation of TpFABP in T. pisiformis tapeworm and cysticercus. The yellowish-brown tint shows the TpFABP protein location. (A) negative sera in cysticercus; (B) antisera in cysticercus; (C) negative sera in adult tapeworm; (D) antisera in adult tapeworm. Arrows indicate the areas of the parasite: MT, microthrix; DC, distal cytoplasm; PC, perinuclear cytoplasm; GD, gathering duct; M, microtrichia; ICW, inside the layer of cystic wall; OCW, outer layer of cystic wall; MCW, middle layer of cystic wall. Scale bars: 20 µm.

opencc-zeroJul 2024View details →

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Data for: Effects of testosterone on gene expression are concordant between sexes but divergent across species of Sceloporus lizards

Hormones mediate sexual dimorphism by regulating sex-specific patterns of gene expression, but it is unclear how much of this regulation involves sex-specific hormone levels versus sex-specific transcriptomic responses to the same hormonal signal. Moreover, transcriptomic responses to hormones can evolve, but the extent to which hormonal pleiotropy in gene regulation is conserved across closely related species is not well understood. We addressed these issues by elevating testosterone levels in juvenile females and males of three Sceloporus lizard species prior to sexual divergence in circulating testosterone, then characterizing transcriptomic responses in the liver. In each species, more genes were responsive to testosterone in males than in females, suggesting that early developmental processes prime sex-specific transcriptomic responses to testosterone later in life. However, overall transcriptomic responses to testosterone were concordant between sexes, with no genes exhibiting sex-by-treatment interactions. By contrast, hundreds of genes exhibited species-by-treatment interactions, particularly when comparing distantly related species with different patterns of sexual dimorphism, suggesting evolutionary lability in gene regulation by testosterone. Collectively, our results indicate that early organizational effects may lead to sex-specific differences in the magnitude, but not the direction, of transcriptomic responses to testosterone, and that the hormone-genome interface accrues regulatory changes over evolutionary time.

Fig. 4 in Expressed sequence tags in venomous tissue of Scorpaena plumieri (Scorpaeniformes: Scorpaenidae)

Fig. 4. Sequence alignment of putative lectin from Scorpaena plumieri. Alignment of a lectin-like EST in silico translated sequence from S. plumieri (ClustalW2 EBI) with fish-egg lectin from Oplegnathus fasciatus (BAL618145), Dicentrarchus labrax (CBK52298), Maylandia zebra (XP_004574029), and Oreochromis niloticus (XP003443389). The recombinant clone was isolated with antibody fraction derived from S. plumieri venom. * identifies and identical residue;: identifies a conserved residue. Underlined residues represent invariable sites, underlined IRLS = N-acetylation site.

Fig. 3 in Expressed sequence tags in venomous tissue of Scorpaena plumieri (Scorpaeniformes: Scorpaenidae)

Fig. 3. The classification of EST from Scorpaena plumieri based on their putative fractions. Three-hundred fifty-six EST edited sequences were initially analyzed with Blast and Swiss protein databanks. The consensus sequence was attributed a function based on the strongest match.

Fig. 2 in Expressed sequence tags in venomous tissue of Scorpaena plumieri (Scorpaeniformes: Scorpaenidae)

Fig. 2. Agarose gel electrophoresis of DNA isolated from clones. White colonies containing insert were grown and the plasmidial DNA isolated and digested with EcoRI enzyme. An aliquot from each clone (1-27) was electrophoresed on 1% Agarose gel and stained with ethidium bromide.

Fig. 1 in Expressed sequence tags in venomous tissue of Scorpaena plumieri (Scorpaeniformes: Scorpaenidae)

Fig. 1. Agarose- formaldehyde electrophoresis of RNA from Scorpaena plumieri. A) 1) 2 µg of E. coli tRNA; 2) 2 µg de rRNA de Rattus norvegicus; 3) and 4) 2 µg total RNA from S. plumieri spine gland. B) 1) 2 µg de total RNA from S. plumieri; 2) the same sample incubated 2 h a 37ºC before electrophoresis.

Figure 4 in Differential gene expression pattern and plasma sex steroids during testicular development in Genyatremus luteus (Perciforme: Haemulidae) (Bloch, 1790)

Figure 4. Principal component analysis (PCA) used to classify the influence of lhr and er gene expression, plasma steroids (11-KT, 17- OHP and E2), ichthyological parameters and GSI on male G. luteus individuals. Legend: LHR = LH receptor; ER = estrogen receptor; KT = 11-ketotestosterone; E2 = 17β-estradiol; OHP = 17-α-hydroxyprogesterone; TW = total weight; TL = total length; GW = gonad weight; GSI = gonadosomatic index.

Figure 1 in Differential gene expression pattern and plasma sex steroids during testicular development in Genyatremus luteus (Perciforme: Haemulidae) (Bloch, 1790)

Figure 1. Photomicrographs of germ cell and testes development stages of Genyatremus luteus. Stages were determined as (A) Immature, (B) Maturing, (C) Mature. Abbreviations are as follows: SPG, spermatogonia; SPC, spermatocyte; SPZ, spermatozoa. All panels were at 60x magnification.

Figure 4 in Saccharomyces cerevisiae OS303 expression of an alkaline protease from a newly isolated Bacillus subtilis D9

Figure 4. Analysis of expression cloning vector (digested and purified cloning vector pRS426/GAL1p-207-Glu-MS ligated with alkaline protease called in this study pRS426/GAL1p-207-Glu-MS/ alkaline-protease plasmids. Lane 1: DNA size marker hyperladder I; Lane 2: pRS426/GAL1p-207-Glu-MS/alkaline-protease linear plasmid around 8000bp; Lane 3: pRS426/GAL1p-207-Glu-MS vector. Lane 4: pRS426/GAL1p-MS vector.

Figure 1 in Saccharomyces cerevisiae OS303 expression of an alkaline protease from a newly isolated Bacillus subtilis D9

Figure 1. The electrophoresis of PCR product of alkaline protease gene from Bacillus subtilis D9. After PCR, 5Μl of the product was run on 1% agarose gel electrophoresis. The expected band of approximately1300bp was observed. Lane 1: DNA size marker hyperladder I; Lane 2: PCR product sample.

Figure 2 in Differential gene expression pattern and plasma sex steroids during testicular development in Genyatremus luteus (Perciforme: Haemulidae) (Bloch, 1790)

Figure 2. Steroid concentrations in the blood plasma of male Genyatremus luteus individuals during their reproductive cycle. (A) 11-ketotestosterone. (B) 17 α-hidroxy progesterone. (C) 17β-estradiol. Data are represented as mean ± SEM. abc: indicates statistically significant difference (p<0.05).

Figure 9 in Evaluation of the Chilli veinal mottle virus CP gene expressing transgenic Nicotiana benthamiana plants for disease resistance against the virus

Figure 9. ELISA plate readings (O.D at 405nm) of leaf samples of transgenic lines (T1-T8) and control plants after 15 dpi.