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Supplementary material 1 from: Pansarin ER, Menezes ELF (2023) A new remarkable Vanilla Mill. (Orchidaceae) species endemic to the Espinhaço Range, Brazil: its phylogenetic position and evolutionary relationships among Neotropical congeners. PhytoKeys 227: 151-165. https://doi.org/10.3897/phytokeys.227.101963

Species of Vanilla included in the molecular study, their locations, vouchers and GenBank accession numbers

opennotspecifiedDec 2021View details →

Fig. 3 in Specialised metabolites as chemotaxonomic markers of Coptosapelta diffusa, supporting its delimitation as sisterhood phylogenetic relationships with Rubioideae

Fig. 3. The current most likely phylogenetic backbone of Rubiaceae based on nuclear (a), chloroplast (b) and mitochondrial (c) data sensu Rydin et al., (2017) and Wikstrom et al., 2020

FIGURE 4 in Exploring gene sequences and phylogenetic relationships of four terrestrial planarian species (Platyhelminthes; Tricladida; Geoplanidae) in Europe

FIGURE 4. Maximum Likelihood (ML) phylogeny inferred with dataset 3 that contains cox1 and ITS1 sequences concatenated. Tree scale corresponds to the number of substitutions per site. Numbers at nodes reflect the UFB support values proportional to size (showing only values from 70% to 100%). Countries of origin labelled as 3-digit alpha code: ESP (Spain), GBR (UK), NZL (New Zealand), PAN (Panama). Results from species in this study are highlighted in red (Australopacifica atrata), blue (Artioposthia exulans), and green (Marionfyfea adventor).

FIGURE 2. Maximum likelihood phylogeny inferred from cox1 in Exploring gene sequences and phylogenetic relationships of four terrestrial planarian species (Platyhelminthes; Tricladida; Geoplanidae) in Europe

FIGURE 2. Maximum likelihood phylogeny inferred from cox1 sequences (dataset 1). Tree scale corresponds to the number of substitutions per site. Numbers at nodes correspond to the ultrafast bootstrap support values (showing only values from 70% to 100%). Countries of origin labelled as 3-digit alpha code: AUS (Australia), BRA (Brazil), ESP (Spain), FRA (France), GBR (UK), NZL (New Zealand), PAN (Panama). Results from species in this study are highlighted in red (Australopacifica atrata), blue (Artioposthia exulans), and green (Marionfyfea adventor).

FIGURE 3 in Exploring gene sequences and phylogenetic relationships of four terrestrial planarian species (Platyhelminthes; Tricladida; Geoplanidae) in Europe

FIGURE 3. ML phylogeny inferred from ITS1 sequences (dataset 2). Tree scale corresponds to the number of substitutions per site. Numbers at nodes reflect the UFB support values (showing only values from 70% to 100%). Countries of origin labelled as 3-digit alpha code: ESP (Spain), GBR (UK), NZL (New Zealand), PAN (Panama). Results from species in this study are highlighted in red (Australopacifica atrata) and blue (Artioposthia exulans).

FIGURE 1. a in Exploring gene sequences and phylogenetic relationships of four terrestrial planarian species (Platyhelminthes; Tricladida; Geoplanidae) in Europe

FIGURE 1. a, Marionfyfea adventor, the sequenced UK specimen, anterior to the left, length ca 1cm. GenBank Acc. Number OQ789899 (photo HDJ); b, Marionfyfea adventor, the sequenced Brittany specimen, anterior to the right, length ca 1cm. GenBank Acc. Number OQ789900 (photo EM); c, longitudinal section of the copulatory apparatus of the Brittany specimen, anterior to the right (ad = adenodactyls, p = penis). GenBank Acc. Number OQ789900 (photo MÁ-P); d, Artioposthia exulans, a specimen from Ireland collected by Julia Cooper, anterior to the left, damaged posterior, length ca 1.2cm (photo HDJ); e, Australopacifica atrata, specimens collected from Sally Barratt's garden in Manchester, feeding on chicken liver in captivity (photo HDJ); f, Microplana cf. edwardsi, the sequenced specimen from Gloucestershire, anterior to the left, length <1cm. GenBank Acc. Number OQ789902 (photo EM); g, Microplana cf. edwardsi, a sequenced specimen from Cumbria, anterior to the left, length <1cm. GenBank OQ789903 Acc. Number (photo EM); h, Microplana cf. edwardsi, a sequenced specimen from Cumbria, anterior to the left, length <1cm. GenBank Acc. Number OQ789904 (photo EM).

FIGURE 5 in Exploring gene sequences and phylogenetic relationships of four terrestrial planarian species (Platyhelminthes; Tricladida; Geoplanidae) in Europe

FIGURE 5. ML phylogeny inferred with dataset 4 including species from the Microplana genus and using Bipaliinae as outgroup. Tree scale corresponds to the number of substitutions per site. Numbers at nodes reflect the UFB support values being proportional to size (showing only values from 70% to 100%). Results from Microplana cf. edwardsi are highlighted in red. Countries of origin labelled as 3-digit alpha code: BGR (Bulgaria), ESP (Spain), FRA (France), GBR (UK), ITA (Italy) PRT (Portugal). The photographs are of specimens with a small-white morphotype of the numbered species (photos EM).

Supplementary Figure 2 in Exploring gene sequences and phylogenetic relationships of four terrestrial planarian species (Platyhelminthes; Tricladida; Geoplanidae) in Europe

Supplementary Figure 2. BI phylogeny inferred with ITS1 sequences in dataset 2. Numbers at nodes reflect the PP values (showing only values from 0.85 to 1.00). Tree scale corresponds to the number of substitutions per site.

Supplementary Figure 3 in Exploring gene sequences and phylogenetic relationships of four terrestrial planarian species (Platyhelminthes; Tricladida; Geoplanidae) in Europe

Supplementary Figure 3. BI phylogeny inferred with the concatenated dataset (dataset 3). Numbers at nodes reflect the PP values (showing only values from 0.85 to 1.00). Tree scale corresponds to the number of substitutions per site.

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Fig. 1. Phylogenetic relationship between SbDGAT1-1 and SbDGAT1-2 with other DGAT1 and DGAT2 in Identification and functional characterization of two acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) genes from forage sorghum (Sorghum bicolor) embryo

Fig. 1. Phylogenetic relationship between SbDGAT1-1 and SbDGAT1-2 with other DGAT1 and DGAT2 protein sequences. Phylogenetic tree was constructed using maximum likelihood method (Tamura et al., 2011; Hall, 2013) in Molecular Evolutionary Genetics Analysis (MEGA) 7.0 tool (Kumar et al., 2016). Bootstrap values are mentioned in the branches. GenBank accession numbers are indicated with species names of the DGAT proteins.

Fig. 11 in The Male Hindwing Costal Roll in Cochylina (Lepidoptera: Tortricidae): Morphological Variation, Phylogenetic Distribution, and Relationship to Host Utilization

Fig. 11. Transformation series of the CR across the Cochylina phylogeny. A, CR as a morphological character mapped into main groups of Cochylina phylogeny. Line colors indicate CR presence (green) or absence (red). B, Abouheif′s test of serial independence for CR as a bi-state character (Obs. = 0.59; S. Obs. = 7.32; P-value = 0.01). C, general structure of the hindwing CR in Cochylina. D, CR as a simple sclerotization of the costa with some external scales. E, CR with hairpencil. F, CR with hairpencil and microscales. Abbreviations: es, external scales; hp, hairpencil; ir, internal ridge; ms, microscales; ms1, microscale type I; ms2, microscale type II; r, roll. Figure not drawn to scale.

Fig. 8 in The Male Hindwing Costal Roll in Cochylina (Lepidoptera: Tortricidae): Morphological Variation, Phylogenetic Distribution, and Relationship to Host Utilization

Fig. 8. Plant-herbivore matrix of Cochylina genera vs. plant families. Color of circles represents presence (green) or absence (red) of the CR. Areas of circles are proportional to number of species of Cochylina feeding of each family from 1 to 16.

Fig. 6 in The Male Hindwing Costal Roll in Cochylina (Lepidoptera: Tortricidae): Morphological Variation, Phylogenetic Distribution, and Relationship to Host Utilization

Fig. 6. Scanning electron microscope images of type I microscales. A, transition between microscales in CR of Lorita baccharivora. B, Neocochylis dubitana, C. Cochylis roseana. D, Falseuncaria ruficiliana. E, C. flaviciliana. Scale bars: 10 µm.

Fig. 7 in The Male Hindwing Costal Roll in Cochylina (Lepidoptera: Tortricidae): Morphological Variation, Phylogenetic Distribution, and Relationship to Host Utilization

Fig. 7. Scanning electron microscope images of type II microscales. A, B, Platphalonidia sp. C, Cochylis atricapitana, D, C. epilinana, E, C. bucera, F,Nycthia pimana. G, Neocochylis molliculana. H, Thyraylia nana. Scale bars: 10 µm (A–F); 2 µm (G–H).

Fig. 4 in The Male Hindwing Costal Roll in Cochylina (Lepidoptera: Tortricidae): Morphological Variation, Phylogenetic Distribution, and Relationship to Host Utilization

Fig. 4. Photomicrographs of the right hindwing of Cochylina. A, Eupoecilia angustana. B, Parirazona sp. C, Aethes moribundana. D, Phalonidia contractana. E, Cochylis bucera, F, Lorita baccharivora. G, Diceratura infantana. H, Nycthia pimana. I, L. scarificata. J, Cochylidia heydeniana. Scale bars: 1 mm.

Fig. 2 in The Male Hindwing Costal Roll in Cochylina (Lepidoptera: Tortricidae): Morphological Variation, Phylogenetic Distribution, and Relationship to Host Utilization

Fig. 2. Pontoturania posterana, scanning electron microscope images, left hindwing, upperside. A, general view of CR. B, distal portion of CR. C, base of hairpencil. D, distal part of hairpencil scale. E, same but basal part. F, edge of CR showing base of hairpencil. G, socket of scale of hairpencil. H, section of CR showing type I microscale. I, distal section of CR showing type II microscale and hairpencil inside. Scale bars: 50 µm (A–C); 20 µm (F, H–I); 2 µm (D–E, G). Abbreviations: hp, hairpencil; ir, internal ridge; ms1, microscale type I; ms2, microscale type II.

Fig. 5 in The Male Hindwing Costal Roll in Cochylina (Lepidoptera: Tortricidae): Morphological Variation, Phylogenetic Distribution, and Relationship to Host Utilization

Fig. 5. Scanning electron microscope images of components of the CR and basal lobe of the hindwing. A, CR of Parirazona sp. B, CR of Eupoecilia angustana. C, elongate scales on CR of Cochylichroa atricapitana. D, dorsal view of flat distal part of CR in Cochylis bucera. E, section of CR in Cochylis bucera showing keel- like extension of the costal edge, microscales type II and hairpencil. F, G, internal vestiture of CR in Falseuncaria ruficiliana. H, basal lobe of E. angustana. I, basal lobe of Parirazona sp. Scale bars: 50 µm (A, H); 20 µm (C–D, I); 10 µm (B, E–F); 2 µm (G). Abbreviations: ce, costal edge; hp, hairpencil; ms2, microscale type II.

Fig. 1 in The Male Hindwing Costal Roll in Cochylina (Lepidoptera: Tortricidae): Morphological Variation, Phylogenetic Distribution, and Relationship to Host Utilization

Fig. 1. Pontoturania posterana, right hindwing CR, upper side, general features. A, aspect under stereomicroscope. B, aspect through optical microscope. C, detail of CR, hairpencil andType I microscales. D, distal portion of CR with microscales type II. Scale bars: 1 mm (A–B); 200 µm (C–D).

Fig. 3 in The Male Hindwing Costal Roll in Cochylina (Lepidoptera: Tortricidae): Morphological Variation, Phylogenetic Distribution, and Relationship to Host Utilization

Fig. 3. Pontoturania posterana, scanning electron microscope, CR internal vestiture. A–D, type I microscales. E–F, type II microscales. Scale bars: 50 µm (A); 10 µm (B, E–F); 2 µm (C–D).