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54,099 results for “Expression”
Figure 7 in Evaluation of the Chilli veinal mottle virus CP gene expressing transgenic Nicotiana benthamiana plants for disease resistance against the virus
Figure 7. PCR Products of the hpt gene from T0 transgenic plants. Lane 1-9 are transgenic. Lane 10, +ve control. Lane 11, control (untransformed) plant.
Figure 6. DNA bands from T0 in Evaluation of the Chilli veinal mottle virus CP gene expressing transgenic Nicotiana benthamiana plants for disease resistance against the virus
Figure 6. DNA bands from T0 transgenic and Agro-infilterated plants. Lane 1-10, transgenic plants. Lane 11-13, agro-infilterated plants.
Figure 3 in Evaluation of the Chilli veinal mottle virus CP gene expressing transgenic Nicotiana benthamiana plants for disease resistance against the virus
Figure 3. Symptoms development on propagative host plants after mechanical inoculation with ChiVMV isolate ATIPK. (a) N. tabacum showing mosaic, mottling and vein clearing (b) C. annum (cv. Loungi) displays the symptoms of mottling, mosaic, leaf deformation and vein clearing.
Figure 2 in Evaluation of the Chilli veinal mottle virus CP gene expressing transgenic Nicotiana benthamiana plants for disease resistance against the virus
Figure 2. Symptoms of ChiVMV on chilli leaves collected from Islamabad. (a) Shows mottling and severe vein clearing and distortion. (b) Shows reduced leaf size with mottling and distortion.
Figure 5 in Escherichia coli expression and characterization of -amylase from Geobacillus thermodenitrificans DSM-465
Figure 5. The interaction of amylopectin (PubChem ID - 439207) and alpha-amylase enzyme active site. The left figure is built by Chimera and the right by MOE software.
Figure 4 in Escherichia coli expression and characterization of -amylase from Geobacillus thermodenitrificans DSM-465
Figure 4. Ramachandran plot for validation of alpha-amylase enzyme 3D structure generated by Raptor-X server.
Figure 3. 3 in Escherichia coli expression and characterization of -amylase from Geobacillus thermodenitrificans DSM-465
Figure 3. 3-dimentionsl protein structure of alpha-amylase built by Raptor-X software.α-helices are indicated by red, β-sheet by yellow and random coils by white. The protein structure consists of a single monomer.
Figure 2 in Escherichia coli expression and characterization of -amylase from Geobacillus thermodenitrificans DSM-465
Figure 2. Enzyme kinetics. (A) The effect of temperature on the enzyme stability; (B) determination of KM and Vmax values of purified alpha-amylase using Lineweaver-Burk plot; (C) The effect of reaction mixture temperature on the enzyme activity indicating an optimum temperature of 70°C; (D) The effect of pH on the enzyme activity, showing maximum activity at pH 8.
Figure 1 in Escherichia coli expression and characterization of -amylase from Geobacillus thermodenitrificans DSM-465
Figure 1. SDS-PAGE photograph indicating the expression and purification of alpha amylase. Lane C- control experiment (without gene), Lane-M. Protein marker (ThermoFisher Scientific PageRulerTM Prestained Protein Ladder, 10 kDa to 180 kDa), Lane E, experimental with expression of gene, Lane P, partially purified enzyme alpha amylase. The molecular weight of the purified enzyme was found as 63 kDa on SDS-PAGE.
Pelagomonas calceolata gene expression levels in different nitrogen conditions and differential expression analysis.
<p>These files contains the expression levels and DESeq2 results of <em>Pelagomonas calceolata</em> genes cultivated with different nitrate conditions. Two strains of <em>P. calceolata </em>(RCC100 and RCC697) were cultivated and their RNAs reads were aligned on the predicted genes of <em>P. calceolata</em> RCC100 genome: <a href="https://www.ncbi.nlm.nih.gov/Traces/wgs/CAKKNE01?display=download" rel="nofollow">https://www.ncbi.nlm.nih.gov/Traces/wgs/CAKKNE01?display=download</a></p> <p>The following culture conditions were analysed :</p> <p>882 µM of Nitrate (RCC100 and RCC697) </p> <p>441 µM of Nitrate (RCC100)</p> <p>220 µM of Nitrate (RCC100 and RCC697)</p> <p>50 µM of Nitrate (RCC697)</p> <p>882 µM Cyanate (RCC100)</p> <p>882 µM Ammonia (RCC100)</p> <p>441 µM Urea (RCC100)</p> <p><a href="../api/records/12582059/draft/files/20230427_RCC100-Nitrate_transcriptomes_rawcounts.tsv/content" target="_blank" rel="noopener noreferrer">20230427_RCC100-Nitrate_transcriptomes_rawcounts.tsv</a> : the file contains the raw read counts of RCC100 in 6 culture conditions in triplicate + the gene names = 19 columns.</p> <p><a href="../api/records/12582059/draft/files/20230427_RCC100-Nitrate_transcriptomes_TPM.tsv/content" target="_blank" rel="noopener noreferrer">20230427_RCC100-Nitrate_transcriptomes_TPM.tsv</a> : same data normalized in transcript per kb per million mapped reads (TPM).</p> <p><a href="../api/records/12582059/draft/files/20230427_RCC100-Nitrate_transcriptomes_rawcounts.tsv/content" target="_blank" rel="noopener noreferrer">20230427_RCC697-Nitrate_transcriptomes_rawcounts.tsv</a> : the file contains the raw read counts of RCC697 of 3 culture conditions in triplicate + the gene names = 10 columns.</p> <p><a href="../api/records/12582059/draft/files/20230427_RCC100-Nitrate_transcriptomes_TPM.tsv/content" target="_blank" rel="noopener noreferrer">20230427_RCC697-Nitrate_transcriptomes_TPM.tsv</a> : same data normalized in transcript per kb per million mapped reads (TPM).</p> <p><span>Differential expression analysis (DESeq2) was performed by pairwise comparisons between the standard condition (882 µM nitrate) and low-nitrate conditions (50, 220 or 441 µM nitrate) or changing nitrogen sources (882 µM ammonium, 882 µM cyanate and 441 µM urea). Each DESeq-results_RCCxxx_xxx.tsv file contains 6 columns : <em>P.calceolata </em>gene name, base Mean, log2 Fold Change, standard error value (lfcSE), pvalue and adjusted pvalue (padj).<br></span></p>
Figure 5 in TiO nanoparticles and salinity stress in relation to artemisinin production and ADS and DBR2 expression in Artemisia absinthium L.
Figure 5. The effect of salinity stress and titanium dioxide nanoparticles on the amount of artemisinin in wormwood (the non–identical letters indicate significant difference based on Duncan test P≤ 0.05).
Figure 3 in TiO nanoparticles and salinity stress in relation to artemisinin production and ADS and DBR2 expression in Artemisia absinthium L.
Figure 3. The effect of salinity and titanium dioxide nanoparticles on ADS gene expression in wormwood (non–identical letters indicate significant difference based on Duncan test P≤ 0.05).
Association between Dysregulated Expression of Ca2+ and ROS-Related Gene Pairs and Breast Cancer Patient Survival
<p>This file is composed of two documents:</p> <ul> <li>Supplementary File 1 containing three Excel files with cumulative proportion survival from redox-related genes, calcium-related genes, and redox and calcium-related genes.</li> <li>Supplementary Table 3 including an Excel file with a functional enrichment analysis using redox and calcium correlated genes. Cell cycle regulation (sheet 1) and Cell adhesion and projection (sheet 2) were the biological processes more enriched.</li> </ul> <p>Both supplementary tables belongs to the study <strong>Association between Dysregulated Expression of Ca2+ and ROS-Related Gene Pairs and Breast Cancer Patient Survival</strong>, published in <strong>Molecular Diagnosis and therapy</strong></p>
Figure 2 in TiO nanoparticles and salinity stress in relation to artemisinin production and ADS and DBR2 expression in Artemisia absinthium L.
Figure 2. (A) Gel electrophoresis of RNA extracted from the leaf; (B) Gel electrophoresis of Standard RT–PCR product of 16s rRNA. M: 100bp DNA size marker. Numbered wells are samples. Gel agarose 1%.
Fig. 5 in Successful transcription but not translation or assembly of Solenopsis invicta virus 3 in a baculovirus-driven expression system
Fig. 5. Confirmation of heterologous expression of SINV-3 transcript by amplification of the 3' (A) and 5' termini (B) from RNA templates purified from SINV- 3-transfected Sf21 cells. Regions amplified are illustrated in the genome diagram between (A) and (B). (A) Three plaque preparations (AcSINV-3 CiC, AcSINV-3 CiD, and AcSINV-3 DiD) were separated by centrifugation into soluble and pelleted fractions, treated with DNase I, reverse transcribed, and the 3' end of the genome amplified by PCR. Lane assignments were as follows: 1 = mass marker (bp); 2, 6 = AcSINV-3 CiC; 3, 7 = AcSINV-3 CiD; 4, 8 = AcSINV-3 DiD; 5, 9 = mock infection; 10 = positive control (wild-type virus); 11 = negative control; 12 = non-template control. (B) AcSINV-3 plaque preparations (CiC and DiD) evaluated by PCR of the 5' end of the genome (RNA preparations). Lane assignments were as follows: 1 = mass marker (bp); 2, 3 = DNase treated, reverse transcribed; 4, 5 = without DNase treatment, reverse transcribed; 6, 7 = DNase treated, without reverse transcription; 8, 9 = without DNase treatment, without reverse transcription; 10 = positive control; 11 = negative control; 12 = non-template control. (C) PCR amplification of the entire SINV-3 genome from DNA preparations of AcSINV-3 CiC (lane 2) and AcSINV-3 DiD (lane 3). Lane 1, molecular markers; lane 4, non-template control. (D) Western blot to evaluate translation of the SINV-3 transcript by detection of viral capsid protein 2 (VP2). Lane assignments were as follows: 1 = AcSINV-3 CiC (4 dpi); 2 = AcSINV-3 CiC (5 dpi); 3 = AcSINV-3 CiD (4 dpi); 4 = AcSINV-3 DiD (5 dpi); 5 = mock infection (negative control); 6 = positive control (purified wild-type SINV-3; kDa).
Fig. 3 in Successful transcription but not translation or assembly of Solenopsis invicta virus 3 in a baculovirus-driven expression system
Fig. 3. (A) Plaque assay results for recombinant SINV-3 (AcSINV-3) transfection of Sf21 cells indicating the dilution used for each plate. (B upper panel) Representa- tive plaque with Sf21 cells stained with neutral red 10 d afer transfection (magnified 100 times). (B lower panel) Corresponding mock-infected Sf21 cells (negative control) afer 10 d of exposure. Plaque areas identify infection of insect cells by virus with corresponding cell death.
Fig. 2 in Successful transcription but not translation or assembly of Solenopsis invicta virus 3 in a baculovirus-driven expression system
Fig. 2. (A) pFastBac1_SINV-3 hybrid construct map. Locations of the restriction sites (black hash marks), bacterial transposon Tn7 sites (grey triangles), polyhedrin promoter (angled arrow corresponding to the sequence below), SINV-3 open reading frames (dark closed arrows), and approximate location of the area detected by the polyclonal antibody preparation (pAb) are shown. (B) Verified sequence of the pFastBac1_SINV-3 hybrid construct illustrating the late gene polyhedrin promoter (angled arrow). SINV-3 sequence is in bold font,with pFastBac1 sequence in normal font, and restriction sites are superscripted and corresponding sequences italicized.Underlined sequence represents the inserted late gene polyhedrin core promoter.Analyzed sequences of the 5'and 3'termini and restriction sites were identical to the wild-type virus.
Fig. 1 in Successful transcription but not translation or assembly of Solenopsis invicta virus 3 in a baculovirus-driven expression system
Fig. 1. Schematic of the SINV-3 genome and sub-cloning strategy to assemble the pFastBac1 donor plasmid/SINV-3 construct. (A) Organization of the SINV-3 genome illustrating the 2 ORFs numbered 1 and 2 that encode for non-structural and structural proteins, respectively, and the genome sections sub-cloned. Oligonucleotide primers used to generate cDNA and amplify each section are indicated. Primers with introduced restriction sites are also indicated. (B) Unique restriction sites for each sub-clone and the assembly process employed to concatenate the entire SINV-3 genome in the pFastBac1 donor vector.
Fig. 5 in Expression profile of two HSP70 chaperone proteins in response to extreme thermal acclimation in Xestia c-nigrum (Lepidoptera: Noctuidae)
Fig. 5. Xc-HSC70 mRNA expression profiles induced by cold (−7 to 5 °C) and heat (37 to 47 °C) in 2nd, 3rd, 4th, 5th, and 6th instars and pupae of Xestia cnigrum. The relative quantities indicate the levels of the HSC70 gene transcript normalized against transcript levels of β-actin as an internal standard and compared with the transcript levels of the untreated control at 25 °C. An asterisk indicates a significant difference between the control and heat shock conditions (significant, * P <0.05). The data are denoted as the mean ± SEM (error bar).
Fig. 7 in Expression profile of two HSP70 chaperone proteins in response to extreme thermal acclimation in Xestia c-nigrum (Lepidoptera: Noctuidae)
Fig. 7. Expression levels of 2 HSP70s at different developmental stages relative to expression levels in 2nd instars at 25 °C. The data are denoted as the mean ± SEM (error bar), and the different lowercase or uppercase letters indicate a significant differenwce in the means as assessed using multi-comparison tests (P <0.05).
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.