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6,383 results for “isolates”
Fig. 1 in Insulin-mimetic activity of 23-glycosyl oleanane triterpenoids isolated from Gymnema latifolium
Fig. 1. Chemical structures of isolated compound 1⎯9 from Gymnema latifolium.
Fig. 3 in Insulin-mimetic activity of 23-glycosyl oleanane triterpenoids isolated from Gymnema latifolium
Fig. 3. NOESY (dotted lines) correlations of compound 1 and 7.
Fig. 2 in Nine geranylgeranylated derivatives isolated from the roots of Rhus chinensis Mill.
Fig. 2. Key HMBC correlations of compounds 1–7.
Data for / Comparison of Antifungal Activity of Thymus Vulgaris Essential Oil and Triple Antibiotic Paste Against Candida Albicans Isolated from Root Canal (In Vitro Study)
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Differential gene expression in aortae isolated from mice consuming normal water or that containing polystyrene beads
<p>Microplastics (MP) derived from the degradation of larger polymers or synthesized for commercial purposes are ubiquitous in the environment and have found their way into food supplies and water systems where human consumption is inevitable. We hypothesize that MP ingestion promotes CVD risk and atherosclerosis. To test this idea, we supplied ApoE-/- mice with normal chow and either normal water or that containing polystyrene (PS) beads (0.5 μm in diameter; dose of 1 μg/ml). After 20 weeks of exposure, we evaluated lesion formation and gene expression data in cardiovascular tissue. We observed that mice consuming the PS water had increased lesion size compared to those mice consuming normal water. but no changes in plasma lipids or cytokines. An RNAseq analysis of collected aortae identified 1622 differentially expressed genes between the groups, consisting of 414 that were up-regulated and 1208 that were down-regulated. </p>
Longitudinal Network Changes and Phenoconversion Risk in Isolated REM Sleep Behavior Disorder
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Antibacterial effect of Hibiscus sabdariffa (Roselle) extract in synergism with voriconazole and fluconazole against fluconazole-resistant Candida albicans isolates: An in vitro study.
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In vitro Effects of Tigecycline in Combination with Other Antimicrobials against Multidrug-Resistant Acinetobacter baumannii Isolates
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Genome sequencing and assembly of Phytophthora oleae, isolate VK10A, NCBI sequences annotation.
<p>Annotation of the first draft of genome assembly of Phytophthora oleae, isolate code: VK10A.</p> <p>This isolate has been found in rot of mature olive drupes (Olea europaea var. sylvestris), from a tree located in Sicily: Complesso Speleologico Villasmundo S. Alfio Nature Reserve (NR) (Melilli, Siracusa, Italy); DATUM WGS 84, 37°13'17.2"N 15°06'22.3"E.</p> <p>Supplementary materials related to the manuscript: </p> <p><strong>Genome sequencing and assembly of <em>Phytophthora oleae</em>, isolate VK10A, causative agent of rot of olive drupes.</strong></p> <p><span>The Whole Genome Shotgun (WGS) of <em>P. oleae</em> genome assembly project has been deposited at DDBJ/ENA/GenBank under the accession JBIMZQ000000000 (assembly DJ1425, https://www.ncbi.nlm.nih.gov/datasets/genome/GCA_046127205.1/).</span></p> <p> </p>
Data from: Population genetics of a broadcast-spawning coral across a tropical-temperate transition zone reveals regional differentiation and isolation of high-latitude reefs
<p>Aim: Genetic connectivity is a key component of species resilience to climate change in terms of recovery capacity following disturbance and capacity to disperse to novel locations as the climate warms and isotherms shift poleward. We aimed to strengthen our understanding of resilience in this context by characterizing patterns of connectivity and genetic diversity in a broadcast spawning coral across a tropical-temperate transition zone. We hypothesize genetic differentiation between tropical and temperate populations and decreasing genetic diversity with higher latitudes.</p> <p>Location: Western Australia (WA).</p> <p>Taxon: <i>Turbinaria </i>species complex. <i>Turbinaria reniformis </i>Oken, 1815 (Dendrophylliidae).</p> <p>Methods: Samples from 930 target corals were collected from ten locations between 13 - 32<sup> o</sup> latitude spanning a 9° C mean temperature range. <i>In-situ</i> species identification of <i>T. reniformis </i>is hindered by morphological plasticity and homoplasy with sister species. We<i> </i>combined Sanger sequencing of two mitochondrial DNA markers and high-throughput genotyping by sequencing (GBS) to isolate a single genetic <i>Turbinaria</i> lineage from our dataset through which patterns of genetic flow and diversity along the WA coastline could be explored using population- and individual-based analyses.</p> <p>Results: Mitochondrial DNA sequence variation was low among <i>Turbinaria</i> samples and could not resolve individual species. Using GBS, we identified three genetically distinct lineages. Subsequent analyses within one of these lineages revealed strong spatial subdivision with 2-3 genetic clusters. While temperate populations were genetically diverged from more tropical sites, we did not observe declines in genetic diversity with latitude.</p> <p>Main Conclusions: Temperate coral populations in Western Australia are genetically isolated from their tropical counterparts. Tropical populations of <i>T. 'reniformis' </i>exhibit adequate connectivity.<i> </i>Shark Bay represents the current southern limit of the tropical population of <i>T. 'reniformis'</i>. Interestingly, temperate <i>T. 'reniformis'</i> in this study exhibit some genetic resilience due to their relatively high genetic diversity, yet the strong patterns of genetic subdivision for this widely dispersing coral species potentially limit their resilience to future climate scenarios.</p>
Figure 1 from: Fadhilah QG, Santoso I, Maryanto AE, Abdullah S, Yasman Y (2021) Evaluation of the antifungal activity of marine actinomycetes isolates against the phytopathogenic fungi Colletotrichum siamense KA: A preliminary study for new antifungal compound discovery. Pharmacia 68(4): 837-843. https://doi.org/10.3897/pharmacia.68.e72817
Figure 1 Results of the antibiosis assay of SM14 isolate in PDA filtrate medium. A. control, B. 6 days, C. 9 days, D. 12 days.
Figure 3 from: Fadhilah QG, Santoso I, Maryanto AE, Abdullah S, Yasman Y (2021) Evaluation of the antifungal activity of marine actinomycetes isolates against the phytopathogenic fungi Colletotrichum siamense KA: A preliminary study for new antifungal compound discovery. Pharmacia 68(4): 837-843. https://doi.org/10.3897/pharmacia.68.e72817
Figure 3 Phylogenetic analysis of marine actinomycetes isolates. The neighbor-joining tree of the three marine actinomycetes isolates (SM11, SM14, and SM15) was based on the 16S rRNA gene sequences. Streptomyces albus subsp. albus DSM 40313T is represented as an outgroup. The bootstrap values, based on 1,000 replications, are shown at the nodes; only values above 50% are given. The scale bar indicates 0.0050 substitutions per nucleotide position.
Data from: An isolated white-tailed deer (Odocoileus virginianus) population on St. John, US Virgin Islands shows low inbreeding and comparable heterozygosity to other larger populations
<p><span>This is the first study to document the genetic diversity of the white-tailed deer population on St. John, US Virgin Islands. The island population was founded by a small number of animals, has very limited hunting or predation, and recently experienced a reduction in size following an extended drought in 2015. DNA samples were collected from hair from 23 anesthetized adult deer (13 males, 10 females) ranging in age from 1-8 years (3.36<u>+</u> 1.9 yr) and also from fecal DNA samples, for a total of 42 individuals analyzed for genetic diversity. The St. John deer data set averaged 4.19 alleles per marker and demonstrates the second lowest number of alleles (A) when compared to other populations of <i>Odocoileus virginianus</i> (4.19). Heterozygosity was similar to the other studies (0.54) with little evidence of inbreeding. To explain the level of heterogygosity and lack of inbreeding within the St. John population, three hypotheses are proposed, including the effect of intrinsic biological traits within the population, a recent infusion of highly heterogeneous loci from North American populations, and a consistent level of immigration from a nearby island. Additional work is needed to further understand the genetic history of the St. John and regional deer populations. </span></p>
Distribution. SE Chad, W Sudan, and isolated populations in extreme N Central African Republic. in Bovidae
Distribution. SE Chad, W Sudan, and isolated populations in extreme N Central African Republic.
Distribution. Coastal SE South Africa, with isolated populations in C South Africa. in Bovidae
Distribution. Coastal SE South Africa, with isolated populations in C South Africa.
No link between population isolation and speciation rate in squamate reptiles
<p>Rates of species formation vary widely across the tree of life and contribute to massive disparities in species richness among clades. This variation can emerge from differences in metapopulation-level processes that affect the rates at which lineages diverge, persist, and evolve reproductive barriers and ecological differentiation. For example, populations that evolve reproductive barriers quickly should form new species at faster rates than populations that acquire reproductive barriers more slowly. This expectation implicitly links microevolutionary processes (the evolution of populations) and macroevolutionary patterns (the profound disparity in speciation rate across taxa). Here, leveraging extensive field sampling from the Neotropical Cerrado biome in a biogeographically-controlled natural experiment, we test the role of an important microevolutionary process – the propensity for population isolation – as a control on speciation rate in lizards and snakes. By quantifying population genomic structure across a set of co-distributed taxa with extensive and phylogenetically independent variation in speciation rate, we show that broad-scale patterns of species formation are decoupled from demographic and genetic processes that promote the formation of population isolates. Population isolation is likely a critical stage of speciation for many taxa, but our results suggest that interspecific variability in the propensity for isolation has little influence on speciation rates. These results suggest that other stages of speciation – including the rate at which reproductive barriers evolve and the extent to which newly formed populations persist – are likely to play a larger role than population isolation in controlling speciation rate variation in squamates.</p>
Thainema gen. nov. (Leptolyngbyaceae, Synechococcales): a new genus of simple trichal cyanobacteria isolated from a solar saltern environment in Thailand
<p>Simple trichal types constitute a group of cyanobacteria with an abundance of novel, often cryptic taxa. Here, we investigated material collected from wet surface-soil in a saline environment in Petchaburi Province, central Thailand. A morphological comparison of the isolated strain with similar known species, as well as its phylogenetic and species delimitation analyses based on the combined datasets of other related organisms, especially simple trichal cyanobacteria, revealed that the material of this study represented an independent taxon. Using a multifaceted method, we propose that this material represents a new genus, <i>Thainema</i> gen. nov., belonging to the family Leptolyngbyaceae, with the type species <i>Thainema salinarum</i> sp. nov. This novel taxon shares similar ecological habitats with strains previously placed in the same lineage.</p>
Protocols for the Isolation of Lactic Acid Bacteria and Determination of the Inhibition Diameter
<p>Video demonstrating the protocols for the isolation of lactic acid bacteria and the determination of the inhibition diameter using the spot-of-the-lawn agar test. Training video elaborated within the ArtiSaneFood project.</p>
Fig. 1 in Pseudonocardia lutea sp. nov., a novel actinobacterium isolated from soil in Chad
Fig. 1. Scanning electron micrograph of strain NEAU-G57T grown on ISP 2 agar for 4 weeks at 28 ǪC.
Fig. 3 in MaNgROVIVIRga CUNICULI gen. nov., sp. nov., a moderately halophilic bacterium isolated from bioturbated Red Sea mangrove sediment, and proposal of the novel family MaNgROVIVIRgaCeae fam. nov.
Fig. 3. Scanning electron microscopy image of Mangrovivirga cuniculi R1DC9T (bar, 1 µm).
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.