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FIGURE 4 in Euastacus vesper sp. nov., a new giant spiny crayfish (Crustacea, Decapoda, Parastacidae) from the Great Dividing Range, New South Wales, Australia

FIGURE 4. Euastacus vesper sp. nov., male holotype (OCL 64.74 mm), Cudgegong River, AM P99506. A, anterior cephalothorax, ventral view; B, left first chela, dorsal view; C–D, right first chela, dorsal and ventral views; E, right maxilliped 3; F, cephalothorax, ventral view,

FIGURE 2 in Euastacus vesper sp. nov., a new giant spiny crayfish (Crustacea, Decapoda, Parastacidae) from the Great Dividing Range, New South Wales, Australia

FIGURE 2. Euastacus vesper sp. nov., male holotype (OCL 64.74 mm), Cudgegong River, AM P99506. A, dorsal habitus; B, ventral habitus.

FIGURE 3 in Euastacus vesper sp. nov., a new giant spiny crayfish (Crustacea, Decapoda, Parastacidae) from the Great Dividing Range, New South Wales, Australia

FIGURE 3. Euastacus vesper sp. nov., male holotype (OCL 64.74 mm), Cudgegong River, AM P99506. A, carapace, dorsal view; B, carapace, right lateral view; C, abdomen, right oblique view; D, abdomen, right lateral view; E, telson and uropods, dorsal view.

FIGURE 1 in Euastacus vesper sp. nov., a new giant spiny crayfish (Crustacea, Decapoda, Parastacidae) from the Great Dividing Range, New South Wales, Australia

FIGURE 1. Euastacus vesper sp. nov. A, known distribution; B, male holotype (OCL 64.74 mm), Cudgegong River, AM P99506; C, same, with ectocommensal temnocephalans; D, male paratype, Cudgegong River, ACP1130; E, habitat in upper Cudgegong River, Coricudgy State Forest, NSW.

FIGURE 2 in First record of a new epibionts suctorian ciliate Tokophrya huangmeiensis sp. n. (Ciliophora, Phyllopharyngea) from redclaw crayfish Cherax quadricarinatus von Martens 1868

FIGURE 2. Line drawings of T. huangmeiensis sp. n. (a) Body of T. huangmeiensis suspended on stalk. (b) Epistylis sp. as a substrate to Tokophrya sp. Scale bars: a=60 µm, b=1 mm.

FIGURE 1 in First record of a new epibionts suctorian ciliate Tokophrya huangmeiensis sp. n. (Ciliophora, Phyllopharyngea) from redclaw crayfish Cherax quadricarinatus von Martens 1868

FIGURE 1. Photomicrographs of T. huangmeiensis sp. n. found on redclaw crayfish (a) Cherax quadricarinatus collected from suspected fish farm. (b) Epistylis sp. as a substrate to T. huangmeiensis sp. n.; Abbreviations: Ep = Epistylis, Th = T. huangmeiensis sp. n. (c) Elongated pyramidal shaped and corrugated cell body of T. huangmeiensis sp. n., arrowhead showing macronucleus. (d) Apical part of the cell body showing single contractile vacuole. (e) Apical border of the cell body showing fascicle of finger-like tentacles. (f) Indicating basal plate which was concave up-word and gently dipped inside the cell body, under this basal plate transparent stalk is present with longitudinal striations. (g) Showing junction of Epistylis sp. as a substrate to Tokophrya sp., slightly protrude outward. Scale bars: b=100 µm, c=10 µm, d=5 µm, e=5 µm, f=5 µm, g=3 µm.

FIGURE 3 in First record of a new epibionts suctorian ciliate Tokophrya huangmeiensis sp. n. (Ciliophora, Phyllopharyngea) from redclaw crayfish Cherax quadricarinatus von Martens 1868

FIGURE 3. Phylogenetic tree generated by Bayesian analysis of SSU rDNA sequences of T. huangmeiensis sp. n. and related ciliates. Genbank accession numbers are listed adjacent to species names. Numbers given at nodes of branches are posterior probabilities (BI) and bootstrap values (ML), respectively. Asterisks are shown where values exceeded 95%. Dashes are shown for values under 50%.

opencc-zeroNov 2023View details →

Supplementary material 3 from: Satmari A, Miok K, Ion MC, Zaharia C, Schrimpf A, Pârvulescu L (2023) Headwater refuges: Flow protects Austropotamobius crayfish from Faxonius limosus invasion. NeoBiota 89: 71-94. https://doi.org/10.3897/neobiota.89.110085

Overview of the results from the eDNA analysis for the detection of A. bihariensis/A. torrentium, F. limosus and A. astaci

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Supplementary material 2 from: Satmari A, Miok K, Ion MC, Zaharia C, Schrimpf A, Pârvulescu L (2023) Headwater refuges: Flow protects Austropotamobius crayfish from Faxonius limosus invasion. NeoBiota 89: 71-94. https://doi.org/10.3897/neobiota.89.110085

Results of two samples Welch t-tests for comparisons between species with respect to the geospatial variables in occurrence sites

FIGURE 5 in Cambarus ectopistes sp. nov., a new stream-dwelling crayfish (Decapoda: Cambaridae) from the French Broad, Pigeon, and Nolichucky River watersheds in the Appalachian Mountain region of North Carolina and Tennessee, USA

FIGURE 5. Live color of A) adult male and B) adult female C. ectopistes sp. nov. from the Pigeon River, NC. Photos by Michael Perkins and W.T. Russ (North Carolina Wildlife Resources Commission).

FIGURE 6 in Cambarus ectopistes sp. nov., a new stream-dwelling crayfish (Decapoda: Cambaridae) from the French Broad, Pigeon, and Nolichucky River watersheds in the Appalachian Mountain region of North Carolina and Tennessee, USA

FIGURE 6. Maps (A. relief, B. HUC 10 boundaries) showing the distribution of C. ectopistes sp. nov. in the Pigeon River (PR), French Broad River (FB), Nolichucky River (NOL), and Cane River (CANE) systems. * Denotes the sampling locality on Paint Creek, Tennessee.

FIGURE 3 in Cambarus ectopistes sp. nov., a new stream-dwelling crayfish (Decapoda: Cambaridae) from the French Broad, Pigeon, and Nolichucky River watersheds in the Appalachian Mountain region of North Carolina and Tennessee, USA

FIGURE 3. Phylogenetic representation of the IQTREE-estimated maximum likelihood tree of cytochrome c oxidase I (COI). A dotted box encompasses the clade representing Cambarus ectopistes sp. nov., whereas a solid grey box encompasses the clade we consider to represent Cambarus robustus s.s. Bootstrap values of 50 or greater are shown at associated nodes.

FIGURE 2 in Cambarus ectopistes sp. nov., a new stream-dwelling crayfish (Decapoda: Cambaridae) from the French Broad, Pigeon, and Nolichucky River watersheds in the Appalachian Mountain region of North Carolina and Tennessee, USA

FIGURE 2. Two-dimensional non-metric multidimensional scaling (NMDS) plot showing differences in morphological characters between C. ectopistes sp. nov. (n = 36) and C. robustus s.s. (n = 16) (stress = 0.0686, linear fit R2 = 0.988.) Vectors reflect the strength of correlation of each of eight significant characters in relation to the NMDS axes. Length (L), width (W), depth (D), total carapace length (TCL), carapace width (CW).

FIGURE 4 in Cambarus ectopistes sp. nov., a new stream-dwelling crayfish (Decapoda: Cambaridae) from the French Broad, Pigeon, and Nolichucky River watersheds in the Appalachian Mountain region of North Carolina and Tennessee, USA

FIGURE 4. Cambarus ectopistes sp. nov.: (A) lateral cephalothorax; (B) dorsal cephalothorax; (C) epistome; (D) antennal scale; (E) dorsal right chela; (F) mesial form I gonopod; (G) lateral form I gonopod; (H) annulus ventralis; (I) mesial form II gonopod; (J) lateral form II gonopod. A–G from holotype (NCSM 90193); H from allotype (NCSM 90194); and I–J from morphotype (NCSM 90195).

FIGURE 1 in Cambarus ectopistes sp. nov., a new stream-dwelling crayfish (Decapoda: Cambaridae) from the French Broad, Pigeon, and Nolichucky River watersheds in the Appalachian Mountain region of North Carolina and Tennessee, USA

FIGURE 1. Map showing localities of all specimens examined for this study, including newly sampled material and individuals in the North Carolina Museum of Natural Sciences (NCSM) Non-molluscan Invertebrates Collection originally identified as Cambarus robustus, C. cf. robustus, or C. (Puncticambarus) sp.

FIGURE 8 in Cambarus ectopistes sp. nov., a new stream-dwelling crayfish (Decapoda: Cambaridae) from the French Broad, Pigeon, and Nolichucky River watersheds in the Appalachian Mountain region of North Carolina and Tennessee, USA

FIGURE 8. Intraspecific variation in C. ectopistes sp. nov. occurs most notably in the shape of the rostrum. A, Nolichucky and Cane Rivers; B, French Broad and Pigeon Rivers.

FIGURE 8. Faxonius elix n in Description of a new species of crayfish in the genus Faxonius (Decapoda: Cambaridae) from the Lower Ohio River Drainage, with evidence of glacial influence on the distribution of some crayfish species throughout the Ohio River basin

FIGURE 8. Faxonius elix n. sp.: Male Form I holotype (A–D; H, J, K), morphotype male Form II (E, F), and allotype female (G, I). Lateral aspect of carapace (A); mesial and lateral aspect of Form I male gonopod, respectively (B–C), mesial view of entire gonopod of Form I male (D); mesial and lateral aspect of form II male gonopod, respectively (E–F); ventral aspect of epistome (G); dorsal view of carapace (H); ventral view of female annulus ventralis (I); dorsal view of antennal scale (J); dorsal view of right chelae (K). Photographs by Guenter Schuster.

FIGURE 7 in Description of a new species of crayfish in the genus Faxonius (Decapoda: Cambaridae) from the Lower Ohio River Drainage, with evidence of glacial influence on the distribution of some crayfish species throughout the Ohio River basin

FIGURE 7. Comparative plate of key diagnostic differences between Faxonius jeffersoni and Faxonius elix n. sp. Photos include MI gonopod (left column), rostrum (center column), and mandible (right column) of F. elix n. sp. (top row) and F. jeffersoni (bottom row). Arrows point to diagnostic characteristics for F. elix n. sp. Photographs by Guenter Schuster.

FIGURE 4 in Description of a new species of crayfish in the genus Faxonius (Decapoda: Cambaridae) from the Lower Ohio River Drainage, with evidence of glacial influence on the distribution of some crayfish species throughout the Ohio River basin

FIGURE 4. Phylogenetic analyses of the mitochondrial gene cytochrome oxidase subunit 1 (CO1) using newly generated sequence data and all publicly available Cambaridae sequence data. TOP LEFT: Maximum Likelihood analysis of all Cambaridae with the genus Faxonius highlighted (gray box). Star on the bottom clade indicates the same branch as the phylogeny on the right. Bottom Left: Unrooted phylogenetic network of the genus Faxonius. Blue circle indicates the same clade as the phylogeny on the right. Right: Excerpt from the tree on the left showing the focal group of this study, including F. jeffersoni and F. elix. Black dots above branches indicate maximum likelihood values>95%.

FIGURE 2 A–G in Description of a new species of crayfish in the genus Faxonius (Decapoda: Cambaridae) from the Lower Ohio River Drainage, with evidence of glacial influence on the distribution of some crayfish species throughout the Ohio River basin

FIGURE 2 A–G. Box pots of scaled body measurements of FI male Faxonius jeffersoni and Faxonius elix, new species. Black dots represent outliers. Measurements made to the nearest 0.01mm.