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Fig. 2 in Isolation of ten unrecorded yeasts from soil in Korea

Fig. 2. Neighbor-joining phylogenetic tree based on 26S rRNA gene sequences shows the phylogenetic relationships between the isolated strains and their closest relatives. The sequence of Solicoccozyma keelungensis CBS 10876T was used as the outgroup. Bootstrap values (70%)> are shown at each branch. Bar, 0.05 substitutions per nucleotide position.

Fig. 4 in Isolation of four unrecorded yeasts in the family Filobasidiaceae from soil in Korea

Fig. 4. Neighbor-joining phylogenetic tree based on 26S rRNA gene sequences shows the phylogenetic relationships between the isolated strains and their closest strains of the genus Naganishia. Yamadazyma scolyti NRRL Y-5512T strain was used as the outgroup. Bootstrap values (>70%) are shown at each branch. Bar, 0.01 substitutions per nucleotide position.

Fig. 3 in Isolation and characterization of unrecorded yeast species from Korea in the families Debaryomycetaceae and Piskurozymaceae

Fig. 3. Phylogenetic tree derived from neighbor-joining analysis based on the 26S rDNA gene sequences, showing the placement of strains 20g9-1 in the genus Solicoccozyma. Candida tropicalis ATCC 750T was used as outgroup. Bootstrap values of above 70% are given at nodes based on 1,000 replicates. Bar, 0.05 substitutions per site.

Fig. 1 in Isolation of ten unrecorded yeasts from soil in Korea

Fig. 1. Phase-contrast micrographs of cells from the unrecorded strains isolated in this study. Strains: A, 20n15-2; B, 20n6-6; C, 20n8-2; D, 20n32-3; E, 20n33-5; F, 20n26-1; G, 20n23-1; H, 20n29-2; I, 20n29-4; J, 20n29-5.

Fig. 2 in Isolation and characterization of unrecorded yeast species from Korea in the families Debaryomycetaceae and Piskurozymaceae

Fig. 2. Phylogenetic tree derived from neighbor-joining analysis based on the 26S rDNA gene sequences, showing the placement of strain YL1 in the Debaryomyces and YL9 in the Candida saitoana. Saitozyma paraflava CBS 10100T was used as outgroup. Bootstrap values of above 70% are given at nodes based on 1,000 replicates. Bar, 0.05 substitutions per site.

Fig. 3 in Isolation of four unrecorded yeasts in the family Filobasidiaceae from soil in Korea

Fig. 3. Neighbor-joining phylogenetic tree based on 26S rRNA gene sequences shows the phylogenetic relationships between the isolated strains and their closest strains of the genus Goffeauzyma. Dothiora cannabinae CBS 737.71T strain was used as the outgroup. Bootstrap values (>70%) are shown at each branch. Bar, 0.01 substitutions per nucleotide position.

Fig. 1 in Isolation and characterization of unrecorded yeast species from Korea in the families Debaryomycetaceae and Piskurozymaceae

Fig. 1. Photomicrographs showing budding cells of strains YL1 (a), YL9 (b) and 20g9-1 (c). All strains were grown for 3 days on YM and YNB agar.

Fig. 3 in Isolation of ten unrecorded yeasts from soil in Korea

Fig. 3. Neighbor-joining phylogenetic tree based on 26S rRNA gene sequences shows the phylogenetic relationships between the isolated strains and their closest relatives. The sequence of Sarocladium mycophilum CBS 166.92T was used as the outgroup. Bootstrap values (70%)> are shown at each branch. Bar, 0.05 substitutions per nucleotide position.

Fig. 2 in Isolation of four unrecorded yeasts in the family Filobasidiaceae from soil in Korea

Fig. 2. Neighbor-joining phylogenetic tree based on 26S rRNA gene sequences shows the phylogenetic relationships between the isolated strains and their closest strains of the genus Cryptococcus. Rhodotorula babjevae CBS 7808T strain was used as the outgroup. Bootstrap values (>70%) are shown at each branch. Bar, 0.01 substitutions per nucleotide position.

Fig. 2 in A report of 20 unrecorded bacterial species isolated from the coastal area of Korean islands in 2022

Fig. 2. Transmission electron micrographs of cells of the strains isolated in this study. Strains: 1. IMCC43116 (0.2 μm); 2. IMCC43200 (0.2 μm); 3. IMCC43225 (0.5 μm); 4. IMCC43240 (0.2 μm); 5. IMCC43252 (0.5 μm); 6. IMCC43266 (0.2 μm); 7. IMCC43345 (0.5 μm); 8. IMCC43261 (0.5 μm); 9. IMCC43586 (0.2 μm); 10. IMCC33673 (0.2 μm); 11. IMCC33772 (0.5 μm); 12. IMCC33842 (0.2 μm); 13. IMCC43611 (0.2 μm); 14. IMCC43617 (0.5 μm); 15. IMCC43655 (0.5 μm); 16. IMCC43657 (0.5 μm); 17. IMCC43666 (0.5 μm); 18. IMCC43670 (0.5 μm); 19. IMCC33788 (0.5 μm); 20. IMCC33805 (0.2 μm). Scale bars are indicated in parenthesis after strain ID.

Fig. 3 in A report of 18 unrecorded prokaryotic species isolated from the feces of an Oriental stork (Ciconia boyciana), and from the intestinal tracts of a cobitid fish (Kichulchoia multifasciata) and a Korean splendid dace (Coreoleuciscus splendidus)

Fig. 3. Phylogenetic tree based on 16S rRNA gene sequence comparisons, showing the relationship between the seven strains belonging to the phylum Firmicutes isolated in this study and the notable type species from each genus. The tree was reconstructed using neighbor joining (NJ), maximum likelihood (ML), and maximum parsimony (MP) algorithms. Filled diamonds indicate corresponding branches present in the phylogenetic tree generated using the three different tree construction methods. Numbers at the nodes represent bootstrap values of more than 70% are shown, based on 1000 replicates (NJ/MP/ML). Limnochorda pilosa HC45T (AP014924) was used as the outgroup. The bar indicates 0.02 accumulated substitutions per nucleotide.

Fig. 4 in A report of 18 unrecorded prokaryotic species isolated from the feces of an Oriental stork (Ciconia boyciana), and from the intestinal tracts of a cobitid fish (Kichulchoia multifasciata) and a Korean splendid dace (Coreoleuciscus splendidus)

Fig. 4. Phylogenetic tree based on 16S rRNA gene sequence comparisons, showing the relationship between the two strains belonging to the phylum Proteobacteria isolated in this study and the notable type species from each family. The tree was reconstructed using neighbor joining (NJ), maximum likelihood (ML), and maximum parsimony (MP) algorithms. Filled diamonds indicate corresponding branches present in the phylogenetic tree generated using the three different tree construction methods. Numbers at the nodes represent bootstrap values of more than 70% are shown, based on 1000 replicates (NJ/MP/ML). Bacteroides fragilis NCTC 9343T (NR_074784.2) was used as the outgroup. The bar indicates 0.05 accumulated substitutions per nucleotide.

Fig. 4. Neighbor-joining phylogenetic tree reconstructed from a in Isolation and characterization of four unrecorded wild yeasts from the soils of Republic of Korea in winter

Fig. 4. Neighbor-joining phylogenetic tree reconstructed from a comparative analysis of 26S rRNA gene sequences showing the relationships of strain NH33 with closely related species. Bootstrap values (>50%) based on neighbor-joining methods are shown at the branch nodes. Bar, 0.01 substitutions per nucleotide position.

Fig. 1 in Isolation and characterization of four unrecorded wild yeasts from the soils of Republic of Korea in winter

Fig. 1. Morphology of cells from the unrecorded strains incubated at 10℃. The colonies of Leucosporidium scottii NH19 (A), Holtermanniella wattica NH20 (B), Buckleyzyma aurantiaca NH33 (C), and Mrakia aquatica YP416 (D). The budding cells of Leucosporidium scottii NH19 (F), Holtermanniella wattica NH20 (G), Buckleyzyma aurantiaca NH33 (H), and Mrakia aquatica YP416 (I). Bars, 20 μm. All strains were grown after 3 days on YM agar.

Fig. 3. Neighbor-joining phylogenetic tree reconstructed from a in Isolation and characterization of four unrecorded wild yeasts from the soils of Republic of Korea in winter

Fig. 3. Neighbor-joining phylogenetic tree reconstructed from a comparative analysis of 26S rRNA gene sequences showing the relationships of strain NH19 with closely related species. Bootstrap values (>50%) based on neighbor-joining methods are shown at the branch nodes. Bar, 0.01 substitutions per nucleotide position.

Fig. 1 in A report of 20 unrecorded bacterial species isolated from the coastal area of Korean islands in 2022

Fig. 1. The maximum-likelihood (ML) phylogenetic tree based on 16S rRNA gene sequences showing the relationship between the strains isolated in this study and their closest bacterial species. Bootstrap values over 70% are shown at nodes for ML, neighbor-joining (NJ), and minimum evolution (ME) methods, respectively (ML/NJ/ME; -, less than 70%). Tree was rooted with Dehalococcoides mccartyi KCTC 15142T (not shown; GenBank accession no. AX814128). Filled circles indicate that the corresponding node was also recovered in the trees reconstructed with both the NJ and ME algorithms, while open circles indicate that the corresponding node was recovered in the tree generated with only one of these algorithms. Scale bar = 0.05 substitutions per nucleotide position.

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Fig. 1 in A report on 20 unrecorded bacterial species of Korea isolated from soil in 2021

Fig. 1. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images of cells of the unrecorded strains isolat- ed in this study. Images 10 and 14 are TEM images, the rest are SEM images. The strains were cultured at their optimal growth conditions. (1) EM0341, (2) EM0359, (3) EM0662, (4) EM0665, (5) EM1075, (6) EM1106, (7) EM1152, (8) EM1158, (9) EM1187, (10) FL16, (11) JN1309, (12) JN2214, (13) NC19, (14) NSM-1, (15) RB28, (16) RM1, (17) WD4, (18) WR2, (19) WS2, (20) WS11.

Fig. 2 in A report of 18 unrecorded prokaryotic species isolated from the feces of an Oriental stork (Ciconia boyciana), and from the intestinal tracts of a cobitid fish (Kichulchoia multifasciata) and a Korean splendid dace (Coreoleuciscus splendidus)

Fig. 2. Phylogenetic tree based on 16S rRNA gene sequence comparisons, showing the relationship between the eight strains belonging to the phylum Actinobacteria isolated in this study and the notable type species from each genus. The tree was reconstructed using neighbor joining (NJ), maximum likelihood (ML), and maximum parsimony (MP) algorithms. Filled diamonds indicate corresponding branches present in the phylogenetic tree generated using the three different tree construction methods. Numbers at the nodes represent bootstrap values of more than 70% are shown, based on 1000 replicates (NJ/MP/ML). Akkermansia muciniphila ATCC BAA-835T (NR_074436.1) was used as the outgroup. The bar indicates 0.02 accumulated substitutions per nucleotide.

Fig. 1 in A report of 18 unrecorded prokaryotic species isolated from the feces of an Oriental stork (Ciconia boyciana), and from the intestinal tracts of a cobitid fish (Kichulchoia multifasciata) and a Korean splendid dace (Coreoleuciscus splendidus)

Fig. 1. Transmission electron micrographs of the strains isolated in this study. The arrows indicate flagella. Strains: 1, H11M7; 2, H11M9; 3, H23M10; 4, H21T1; 5, H13T1; 6, H43T7; 7, H21T20; 8, H23M25; 9, H23M9; 10, S13R1; 11, H13R26; 12, H11M5; 13, H11M15; 14, H11R12; 15, H21T7; 16, H11R21; 17, M13M1; 18, H23T21.