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FIGURE 5 in The identity of Aplocheilus andamanicus (Köhler, 1906) (Teleostei: Cyprinodontiformes), an endemic Killifish from the Andaman Islands, with notes on Odontopsis armata van Hasselt
FIGURE 5. Phylogeographical map showing the distribution of Aplocheilus andamanicus, A. panchax and A. armatus.
FIGURE 4 in The identity of Aplocheilus andamanicus (Köhler, 1906) (Teleostei: Cyprinodontiformes), an endemic Killifish from the Andaman Islands, with notes on Odontopsis armata van Hasselt
FIGURE 4. Phylogenetic position of Aplocheilus andamanicus, A. panchax and A. armatus based on Bayesian analysis. Bayesian topology is shown. Values along the nodes are Bayesian posterior probabilities followed by the bootstrap support from maximum likelihood analysis based on best partition scheme. Cubanichthys pengelleyi is used as the out-group. Sequences of topotypes of Aplocheilus andamanicus, A. panchax and A. armatus are labelled in red.
FIGURE 3. Diagnostic characters separating Aplocheilus andamanicus and A in The identity of Aplocheilus andamanicus (Köhler, 1906) (Teleostei: Cyprinodontiformes), an endemic Killifish from the Andaman Islands, with notes on Odontopsis armata van Hasselt
FIGURE 3. Diagnostic characters separating Aplocheilus andamanicus and A. panchax. (A) Diagrammatic representation of the frontal squamation pattern and neuromasts and (B) structural morphology and coloration pattern of caudal fin in Aplocheilus andamanicus (BNHS FWF 563) and A. panchax (BNHS FWF 569). Abbreviations: ais = anterior infraorbital series; an = anterior naris; arn = anterior rostral neuromast; ots = otic series; pan = parietal neuromast; pbs = preorbital series; pis = posterior infraorbital series; pn = posterior naris; prn = posterior rostral neuromast; sos = supraorbital series; stn = supratemporal neuromasts; A–F = frontal scales.
Supplementary material 3 from: Bragança PHN, Amorim PF, Costa WJEM (2018) Pantanodontidae (Teleostei, Cyprinodontiformes), the sister group to all other cyprinodontoid killifishes as inferred by molecular data. Zoosystematics and Evolution 94(1): 137-145. https://doi.org/10.3897/zse.94.22173
Maximum Likelihood (A) and Bayesian Inference (B) analysis including the 16S gene. : Explanation note: Maximum Likelihood (A) and Bayesian Inference (B) analysis including segments of the nuclear genes ENC1, GLYT, MYH6, SH3PX3, RAG1, the mitochondrial genes 16S and first and second codon position of the COI. In ML, bootstrap values under 50 are not represented, and posterior probability values under 0.5 are not represented in BI.
Supplementary material 5 from: Bragança PHN, Amorim PF, Costa WJEM (2018) Pantanodontidae (Teleostei, Cyprinodontiformes), the sister group to all other cyprinodontoid killifishes as inferred by molecular data. Zoosystematics and Evolution 94(1): 137-145. https://doi.org/10.3897/zse.94.22173
Bayesian Inference tree : Explanation note: Bayesian inference tree comprising segments of the nuclear genes ENC1, GLYT, MYH6, SH3PX3, RAG1 and first and second codon position of the mitochondrial gene COI (5,083 bp). Numbers on each node are posterior probablity values; values under 50 are not present in the tree.
Annotation file (gff3) from the killifish, Fundulus rathbuni (gill epithelium)
<p>Annotation, gff3 file of the reference de novo transcriptome assembly from the killifish, Fundulus rathbuni created with the dammit pipeline using the Pfam, Rfam, OrthoDB databases in addition to Fundulus heteroclitus amino acids (RefSeq).</p> <p>Fish were acclimated to either brackish or fresh water then exposed to an acute brackish water challenge. Transcriptome data from gill epithelium tissue were collected. A reference transcriptome assembly was generated from all individuals then used to analyze transcriptional responses to salinity.</p> <p> </p>
Fig. 3 in Time-calibrated molecular phylogeny reveals a Miocene-Pliocene diversification in the Amazon miniature killifish genus Fluviphylax (Cyprinodontiformes: Cyprinodontoidei)
Fig. 3 Time-calibrated phylogeny of the Cyprinodontoidei and Fluviphylax obtained from the Bayesian dating analysis in Beast v.1.8. Bars represent maximum and minimum date estimates for each node, and the numbers are node divergence mean ages. Arrows indicate the nodes
Fig. 1 in Time-calibrated molecular phylogeny reveals a Miocene-Pliocene diversification in the Amazon miniature killifish genus Fluviphylax (Cyprinodontiformes: Cyprinodontoidei)
Fig. 1 Distribution map of Fluviphylax: F. obscurus (white dot), F. palikur (orange dot), F. pygmaeus (yellow dot), F. simplex (blue dot), F. zonatus (red dot), Fluviphylax sp. A (green dot), Fluviphylax sp. B (brown dot), and Fluviphylax sp. C (purple dot)
Fig. 2 Phylogenetic relationships among 30 in Time-calibrated molecular phylogeny reveals a Miocene-Pliocene diversification in the Amazon miniature killifish genus Fluviphylax (Cyprinodontiformes: Cyprinodontoidei)
Fig. 2 Phylogenetic relationships among 30 species of Cyprinodontoidei, including all nominal species of Fluviphylax and three undescribed species, inferred by using partial sequences of the nuclear-encoded genes GLYT1, ENC1, RAG1, MYH6, and SREB2 and the mitochondrial gene COI, total of 5880 bp. Numbers left to the bar indicate posterior
TABLE 1 in Range extension of the Honduran endemic killifish Tlaloc portillorum (Matamoros & Schaefer 2010) (Cyprinodontiformes: Profundulidae): new records from the upper reaches of the Patuca and Choluteca Rivers
<p><b>TABLE 1.</b> Table with geographic information, for each sample included in Figure one. Type series as in Matamoros & Schaefer, 2010; The source Museum collections LSUMZ= Louisiana State University Museum Zoology, collection of fishes; USM= University of Southern Mississippi, collection of fishes.</p><table><tbody><tr><th><b>ID</b></th><th><b>City or Town</b></th><th><b>Basin/Sub-basin</b></th><th><b>Longitude</b></th><th><b>Latitude</b></th><th><b>Altitude (m)</b></th><th><b>Source</b></th></tr></tbody><tbody><tr><th>1</th><td>Siguatepeque</td><td>Ulúa-Calam</td><td>-87.876</td><td>14.533</td><td>1368</td><td>Holotype/USM31400</td></tr><tr><th>2</th><td>Siguatepeque</td><td>Ulúa-Calam</td><td>-87.876</td><td>14.533</td><td>1368</td><td>Paratypes/USM31589</td></tr><tr><th>3</th><td>Siguatepeque</td><td>Ulúa-Calam</td><td>-87.878</td><td>14.554</td><td>1368</td><td>Paratypes/USM31598</td></tr><tr><th>4</th><td>Siguatepeque</td><td>Ulúa-Calam</td><td>-87.878</td><td>14.554</td><td>1368</td><td>Paratypes/USM31592</td></tr><tr><th>5</th><td>Siguatepeque</td><td>Ulúa-Calam</td><td>-87.881</td><td>14.541</td><td>1414</td><td>Paratypes/USM31597</td></tr><tr><th>6</th><td>Ojojona</td><td>Nacaome-Verdugo</td><td>-87.294</td><td>13.928</td><td>1391</td><td>Salgado-Maldonado <i>et al</i>. 2015</td></tr><tr><th>7</th><td>Lepaterique</td><td>Nacaome-Verdugo</td><td>-87.466</td><td>14.061</td><td>1462</td><td>Salgado-Maldonado <i>et al</i>. 2014</td></tr><tr><th>8</th><td>Lepaterique</td><td>Choluteca-Guacerique</td><td>-87.432</td><td>14.070</td><td>1656</td><td>Salgado-Maldonado <i>et al</i>. 2014</td></tr><tr><th>9</th><td>Ojojona</td><td>Nacaome-Verdugo</td><td>-87.294</td><td>13.928</td><td>1391</td><td>Salgado-Maldonado <i>et al</i>. 2014</td></tr><tr><th>10</th><td>Potrerillos</td><td>Ulúa-Selguapa</td><td>-87.881</td><td>14.541</td><td>1450</td><td>Pinacho-Pinacho <i>et al</i>. 2015</td></tr><tr><th>11</th><td>Ojojona</td><td>Nacaome-Verdugo</td><td>-87.318</td><td>13.930</td><td>1575</td><td>LSUMZ16071</td></tr><tr><th>12</th><td>San Sebastian</td><td>Nacaome-Verdugo</td><td>-87.360</td><td>13.962</td><td>1673</td><td>LSUMZ16073</td></tr><tr><th>13</th><td>Ojojona</td><td>Choluteca-Guacerique</td><td>-87.373</td><td>13.989</td><td>1415</td><td>LSUMZ16075</td></tr><tr><th>14</th><td>Lepaterique</td><td>Nacaome-Verdugo</td><td>-87.466</td><td>14.064</td><td>1464</td><td>USM31628</td></tr><tr><th>15</th><td>Lepaterique</td><td>Choluteca-Guacerique</td><td>-87.432</td><td>14.071</td><td>1675</td><td>USM31631</td></tr><tr><th>16</th><td>Siguatepeque</td><td>Ulúa-Selguapa</td><td>-87.870</td><td>14.535</td><td>1347</td><td>USM45512</td></tr><tr><th>17</th><td>Siguatepeque</td><td>Ulúa-Calam</td><td>-87.875</td><td>14.550</td><td>1337</td><td>USM31652</td></tr><tr><th>18</th><td>Potrerillos</td><td>Ulúa-Canoas</td><td>-87.878</td><td>14.554</td><td>1332</td><td>USM31590</td></tr><tr><th>19</th><td>Ojojona</td><td>Choluteca-Guacerique</td><td>-87.287</td><td>13.954</td><td>1286</td><td>This Publication</td></tr><tr><th>20</th><td>El Aguacatal</td><td>Choluteca-Guacerique</td><td>-87.362</td><td>14.012</td><td>1409</td><td>This Publication</td></tr><tr><th>21</th><td>Tatumbla</td><td>Choluteca-Guacerique</td><td>-87.101</td><td>14.013</td><td>1309</td><td>This Publication</td></tr><tr><th>22</th><td>Tatumbla</td><td>Choluteca-Guacerique</td><td>-87.096</td><td>14.015</td><td>1295</td><td>This Publication</td></tr><tr><th>23</th><td>Tatumbla</td><td>Choluteca-Guacerique</td><td>-87.098</td><td>14.016</td><td>1314</td><td>This Publication</td></tr><tr><th>24</th><td>Tatumbla</td><td>Choluteca-Guacerique</td><td>-87.097</td><td>14.016</td><td>1319</td><td>This Publication</td></tr><tr><th>25</th><td>Tatumbla</td><td>Choluteca-Guacerique</td><td>-87.096</td><td>14.017</td><td>1301</td><td>This Publication</td></tr><tr><th>26</th><td>Tatumbla</td><td>Choluteca-Guacerique</td><td>-87.111</td><td>14.032</td><td>1259</td><td>This Publication</td></tr><tr><th>27</th><td>Tatumbla</td><td>Choluteca-Guacerique</td><td>-87.110</td><td>14.033</td><td>1314</td><td>This Publication</td></tr><tr><th>28</th><td>Tatumbla</td><td>Choluteca-Guacerique</td><td>-87.109</td><td>14.033</td><td>1314</td><td>This Publication</td></tr><tr><th>29</th><td>Tatumbla</td><td>Choluteca-Guacerique</td><td>-87.108</td><td>14.034</td><td>1265</td><td>This Publication</td></tr><tr><th>30</th><td>Tatumbla</td><td>Choluteca-Guacerique</td><td>-87.107</td><td>14.034</td><td>1274</td><td>This Publication</td></tr><tr><th>31</th><td>Tatumbla</td><td>Choluteca-Guacerique</td><td>-87.106</td><td>14.035</td><td>1277</td><td>This Publication</td></tr><tr><th>32</th><td>Tatumbla</td><td>Choluteca-Guacerique</td><td>-87.107</td><td>14.036</td><td>1276</td><td>This Publication</td></tr><tr><th>33</th><td>Lepaterique</td><td>Choluteca-Guacerique</td><td>-87.412</td><td>14.054</td><td>1516</td><td>This Publication</td></tr><tr><th>34</th><td>Lepaterique</td><td>Nacaome-Verdugo</td><td>-87.462</td><td>14.065</td><td>1487</td><td>This Publication</td></tr><tr><th>35</th><td>Tegucigalpa</td><td>Choluteca-Guacerique</td><td>-87.205</td><td>14.084</td><td>942</td><td>This Publication</td></tr><tr><th>36</th><td>Tamara</td><td>Choluteca-El Hombre</td><td>-87.293</td><td>14.175</td><td>1317</td><td>This Publication</td></tr><tr><th>37</th><td>Comayagua</td><td>Ulúa-Humuya Alto</td><td>-87.654</td><td>14.445</td><td>576</td><td>This Publication</td></tr><tr><th>38</th><td>Comayagua</td><td>Ulúa-Humuya Alto</td><td>-87.654</td><td>14.467</td><td>573</td><td>This Publication</td></tr><tr><th>39</th><td>San Francisco de Orica</td><td>Patuca-Guayape</td><td>-86.977</td><td>14.908</td><td>1570</td><td>This Publication</td></tr></tbody></table>
Data from: Functional and population genomic divergence within and between two species of killifish adapted to different osmotic niches
Adaptation to salinity affects species distributions, promotes speciation, and guides many evolutionary patterns in fishes. To uncover the basis of a complex trait like osmoregulation, genome-level analyses are sensible. We combine population genomic scans with genome expression profiling to discover candidate genes and pathways associated with divergence between osmotic environments. We compared transcriptome sequence divergence between multiple freshwater and saltwater populations of the rainwater killifish, Lucania parva. We also compared sequence divergence between L. parva and its sister species, Lucania goodei, a freshwater specialist. We found highly differentiated single nucleotide polymorphisms (SNPs) between freshwater and saltwater L. parva populations in cell junction and ion transport genes, including V-type H+ ATPase. Between species, we found divergence in reproduction and osmotic stress genes. Genes that were differentially expressed between species during osmotic acclimation included genes involved in ion transport and cell volume regulation. Gene sets that were divergent in coding sequence and divergent in expression did not overlap, although they did converge in function. Like many studies using genomic scans, our approach may miss some loci that contribute to adaptation but have complicated patterns of allelic variation. Our study suggests that gene expression and coding sequence may evolve independently as populations adapt to a complex physiological challenge.
Figure 6 in Taxonomic revision of the seasonal killifish genus Nothobranchius from Zanzibar, East Africa (Cyprinodontoidei: Aplocheilidae)
Figure 6. Nothobranchius melanospilus (Pfeffer 1896), live exemplars: (a) UFRJ 6515, male, 32.6 mm SL; (b) UFRJ 6515, female, 31.1 mm SL.
Figure 4 in Taxonomic revision of the seasonal killifish genus Nothobranchius from Zanzibar, East Africa (Cyprinodontoidei: Aplocheilidae)
Figure 4. Geographical distribution of Nothobranchius guentheri (Pfeffer 1893), circles, and Nothobranchius melanospilus (Pfeffer 1896), squares.
Figure 5 in Taxonomic revision of the seasonal killifish genus Nothobranchius from Zanzibar, East Africa (Cyprinodontoidei: Aplocheilidae)
Figure 5. Nothobranchius melanospilus (Pfeffer 1896), BMNH 2016.12.2.1, lectotype, female, 35.2 mm SL.
Figure 3 in Taxonomic revision of the seasonal killifish genus Nothobranchius from Zanzibar, East Africa (Cyprinodontoidei: Aplocheilidae)
Figure 3. Diagrammatic representation of the latero-sensory system and frontal squamation on the dorsal surface of the head in: (a) Nothobranchius melanospilus, UFRJ 6874, male, 39.8 mm SL; (b) Nothobranchius guentheri, UFRJ 8416, male, 34.1 mm SL; asss: anterior section of the anterior supraorbital series; anss: posterior rostral neuromast; psss: posterior section of the anterior supraorbital series; poss: posterior supraorbital series; prn: posterior rostral neuromast. Scale bar: 2 mm.
Figure 2 in Taxonomic revision of the seasonal killifish genus Nothobranchius from Zanzibar, East Africa (Cyprinodontoidei: Aplocheilidae)
Figure 2. Nothobranchius guentheri (Pfeffer 1893), live exemplars: (a) UFRJ 8420, male, 33.1 mm SL; (b) UFRJ 8420, female, 29.3 mm SL.
Figure 2 in Comparative morphology, phylogenetic relationships, and historical biogeography of plesiolebiasine seasonal killifishes (Teleostei: Cyprinodontiformes: Rivulidae)
Figure 2. Jaws, jaw suspensorium, and opercular series: left jaw suspensorium and anterior part of opercular series, lateral view, of Plesiolebias filamentosus (A), and Pituna compacta (B); left autopalatine, medial view, of Kryptolebias brasiliensis (C), Pi. compacta (D), and Maratecoara splendida (E); left maxilla, proximal portion, dorsal view, of Rachovia maculipinnis (F), and Pi. compacta (G); left maxilla, proximal portion, posterior view, of M. splendida (H), and Pl. filamentosus (I). Numbers in brackets are characters and character states numbered according to Appendix S2. Larger stippling indicates cartilage. Scale bars = 0.5 mm.
Figure 4 in Comparative morphology, phylogenetic relationships, and historical biogeography of plesiolebiasine seasonal killifishes (Teleostei: Cyprinodontiformes: Rivulidae)
Figure 4. Neurocranium, vertebrae, and fins: anterior portion of neurocranium, central and left portion, ventral view, of Papiliolebias bitteri (A); median portion of ventral part of neurocranium, left lateral view, of Plesiolebias filamentosus (B), and Maratecoara splendida (C); parasphenoid, ventral view, of Pa. bitteri (D); neurocranium, posterior part, and first two vertebrae, lateral view (ribs excluded), of Pl. filamentosus (E), and Ma. splendida (F); caudal skeleton (fin rays excluded), left lateral view, of Stenolebias damascenoi (G); anal-fin base, anterior portion, left lateral view, of Plesiolebias glaucopterus (H); left post-temporal and supracleithrum, lateral view, of Maratecoara formosa (I), Pa. bitteri (J), and Pterolebias longipinnis (K); left proximal radials of pectoral fin, lateral view, of Pituna poranga (L), and Maratecoara lacortei (M); pelvic bones, dorsal view, of Pituna obliquoseriata (N). Numbers in brackets are characters and character states numbered according to Appendix S2. Larger stippling indicates cartilage. Scale bars = 0.5 mm.
Figure 3 in Comparative morphology, phylogenetic relationships, and historical biogeography of plesiolebiasine seasonal killifishes (Teleostei: Cyprinodontiformes: Rivulidae)
Figure 3. Hyoid and branchial arches: left hyoid bar, lateral view, of Plesiolebias filamentosus (A); basihyal and ventral part of branchial arches (fifth ceratobranchial excluded), central and left portion, dorsal view, of Pl. filamentosus (B), and Pituna poranga (C); urohyal, left lateral view, of Papiliolebias bitteri (D), and Rivulus pictus (E); dorsal branchial arches, anterior portion, ventral view, of Maratecoara splendida (F), and Pi. poranga (G); dorsal branchial arches, posterior portion, ventral view, of M. splendida (H), and Pa. bitteri (I). Numbers in brackets are characters and character states numbered according to Appendix S2. Larger stippling indicates cartilage. Scale bars = 0.5 mm.
Figure 6 in Comparative morphology, phylogenetic relationships, and historical biogeography of plesiolebiasine seasonal killifishes (Teleostei: Cyprinodontiformes: Rivulidae)
Figure 6. Frontal scales (A–H) and cephalic squamation patterns amongst plesiolebiasines: A, Pituna obliquoseriata; B, Maratecoara lacortei; C, Plesiolebias xavantei. Abbreviations: an, anterior supraorbital series, posterior-most neuromast; pn, posterior supraorbital series, anterior-most neuromast. Scale bars = 1.0 mm.
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