Find research datasets worth reusing

Figure 13 in Integrative taxonomy of crustacean y-larvae (Thecostraca: Facetotecta) using laboratory-rearing and molecular analyses of single specimens, with the description of a new vermiform species

Figure 13. Hansenocaris demodex sp. nov., paratypes from Sesoko Island in SEM:, an early nauplius (LSN − 3?) (A–C) and a later nauplius (LSN − 2) with unmoulted later larval stages still inside. A, ventral view; B, lateral view; C, cephalic shield, frontal view; D, ventrolateral view; E, lateral view; F, cephalic shield, frontal view; G, cephalic shield, dorsal view. Abbreviations: a1, first antennae; a2, second antennae; dc sp, dorsocaudal spine; ex, exopod; fur sp, furcal spine; la, labrum; md, mandible. Small Arabic numerals, many annotated with l or r for left and right, respectively, refer to those surface structures that could be matched with those of the LSN (see Table 4). Green overlay, LSN; purple overlay, LSN − 1; red overlay, anterior field of facets. Squares with dotted outline: sample numbers, museum numbers and type status.

Figure 12 in Integrative taxonomy of crustacean y-larvae (Thecostraca: Facetotecta) using laboratory-rearing and molecular analyses of single specimens, with the description of a new vermiform species

Figure 12. Hansenocaris demodex sp. nov., early nauplius (LSN − 5 or LSN − 6?) from Sesoko Island in SEM. A, ventrolateral view; B, lateral view; C, dorsal view; D, frontal view; E, labrum and naupliar appendages, right side; F, naupliar appendages, right side, median view; G, first and second antennae, right side, lateral view, asterisk indicating rudimentary segment of a2 exopod; H, dorsocaudal spine and furcal spines, terminal view. Abbreviations: a1, first antenna; a2, second antenna; ba, basis; co, coxa; dc sp, dorsocaudal spine; en, endopod; ex, exopod; fur sp, furcal spine; F-1 to F-3, frontal plates 1–3; la, labrum complex; md, mandible; rud, rudimentary; W, window plate. Small Arabic numerals, many annotated with l or r for left and right, respectively, refer to those cuticular structures that could be matched with those of the LSN (see Table 4). Yellow overlay, cephalic shield; blue overlay, faciotrunk; red overlay, anterior field of facets. Square with dotted outline: museum number.

Figure 10 in Integrative taxonomy of crustacean y-larvae (Thecostraca: Facetotecta) using laboratory-rearing and molecular analyses of single specimens, with the description of a new vermiform species

Figure 10. Hansenocaris demodex sp. nov., cyprid from Green Island in SEM. A, lateral view; B, cephalic shield, frontal view; C, putative lattice organ, close-up; D, thoracic limbs 1–6; E, thorax, abdomen and telson, dorsal view. Abbreviations: ba, basis; co, coxa; en, endopod; ex, exopod; la, labrum; pro scle, proximal sclerites of thoracopods; thp 1, thoracopod 1; thp 6, thoracopod 6. Small Arabic numerals, many annotated with l or r for left and right, respectively, refer to cuticular structures (see Table 5). Square with dotted outline: sample number and museum numbers. Dotted arrows in D point to long medial setae of thoracopodal endopods.

Figure 9 in Integrative taxonomy of crustacean y-larvae (Thecostraca: Facetotecta) using laboratory-rearing and molecular analyses of single specimens, with the description of a new vermiform species

Figure 9. Hansenocaris demodex sp. nov., holotype (A, B, D, E) and paratype (C) cyprid from Sesoko Island in SEM. A, posterior thoracic segments and anterior abdominal segments, lateral view; B, thoracopods, ventral view; C, telson, dorsall view; D, telson, terminal view; E, right furcal ramus, posterior view. Abbreviations: fur ram, furcal ramus; ba, basis; co, coxa; en, endopod; ex, exopod; pro scle, proximal sclerites of thoracopods; thp 1, thoracopod 1. Roman numerals I–VI – thoracic segments. Large Arabic numerals 1–3 – abdominal segments 1–3. Small Arabic numerals, many annotated with l or r for left and right, respectively, refer to cuticular structures (see Table 5). Square with dotted outlines: sample numbers, museum numbers and type status.

Figure 14 in Integrative taxonomy of crustacean y-larvae (Thecostraca: Facetotecta) using laboratory-rearing and molecular analyses of single specimens, with the description of a new vermiform species

Figure 14. Morphology of four types of y-larvae from Sesoko Island used for molecular analyses (see overview in Table 6), all photographed in life. A, Hansenocaris demodex sp. nov., lecithotrophic LSN (last-stage nauplius) with cyprid inside. B, 'Bumblebee' type, lecithotrophic LSN with cyprid inside. C, 'Big brown' type, lecithotrophic LSN with cyprid inside. D, planktotrophic nauplius, identified as Itô's (1986a) Pacific type I. Squares with dotted outline: sample numbers.

Figure 6 in Integrative taxonomy of crustacean y-larvae (Thecostraca: Facetotecta) using laboratory-rearing and molecular analyses of single specimens, with the description of a new vermiform species

Figure 6. Hansenocaris demodex sp. nov., last-stage nauplius (LSN) from Green Island in SEM. A, lateral view; B, dorsal view. C, ventral view; D, frontal view. Abbreviations: a1, first antenna; a2, second antenna; dc sp, dorsocaudal spine; fur sp, furcal spine; la, labrum; md, mandible. Arabic numerals, many annotated with l or r for left and right, respectively, refer to cuticular structures (see Table 4). Square with dotted outline: sample number and museum number.

Figure 8 in Integrative taxonomy of crustacean y-larvae (Thecostraca: Facetotecta) using laboratory-rearing and molecular analyses of single specimens, with the description of a new vermiform species

Figure 8. Hansenocaris demodex sp. nov., holotype (B, C) and paratypes (A, D), cyprids from Sesoko Island in SEM. A, live specimen in four different positions; B, cephalic shield, frontal view; C, cephalic shield, dorsal view; D, cephalic shield, oblique posterior view. Abbreviations: par occ pro, paraocular process. Small Arabic numerals, many annotated with l or r for left and right, respectively, refer to cuticular structures of the cyprid (see Table 5). Squares with dotted outlines: sample numbers, museum numbers and type status.

Figure 7 in Integrative taxonomy of crustacean y-larvae (Thecostraca: Facetotecta) using laboratory-rearing and molecular analyses of single specimens, with the description of a new vermiform species

Figure 7. Hansenocaris demodex sp. nov., holotype (A, B, D–I) and paratype (C), cyprids from Sesoko Island photographed before drying (A) and in SEM after drying and mounting (B–I). A, lateral view; B, lateral view; C, cephalic region, lateral view; D, cephalic region, ventral view; E, bifid appendix (frontal filaments?); F, labrum; G, pores and sensilla; H, close-up of hook of left first antenna; I, abdomen and telson, dorsal view. Abbreviations: a1, first antenna; a1 hk, first antenna hook; a2 rud, second antenna rudiment; ae, aesthetasc; en, endopod; ex, exopod; la, labrum; md rud, mandible rudiment; par occ pro, paraocular process. Roman numerals I–VI – thoracic segments. Large Arabic numerals 1–3 – abdominal segments. Small Arabic numerals, many annotated with l or r for left and right, respectively, refer to cuticular structures (see Table 5). Square with dotted outline: sample numbers, museum numbers and type status.

Figure 5 in Integrative taxonomy of crustacean y-larvae (Thecostraca: Facetotecta) using laboratory-rearing and molecular analyses of single specimens, with the description of a new vermiform species

Figure 5. Hansenocaris demodex sp. nov., paratype, last-stage nauplius (LSN) from Sesoko Island in SEM. A, lateral view; B, cephalic shield, anterior view; C–J, close-up views of pores and sensilla of cephalic shield and faciotrunk; K–M, labrum and naupliar limbs (a1, a2, md). Abbreviations: a1, first antenna; a2, second antenna; md, mandible; la, labrum; scl, sclerite of a1. Small Arabic numerals, many annotated with l or r for left and right, respectively, refer to cuticular structures (see Table 4). Yellow overlay, cephalic shield; blue overlay, faciotrunk; red overlay, anterior field of facets. Square with dotted outline: sample number, museum number and type status.

Figure 4 in Integrative taxonomy of crustacean y-larvae (Thecostraca: Facetotecta) using laboratory-rearing and molecular analyses of single specimens, with the description of a new vermiform species

Figure 4. Hansenocaris demodex sp. nov., paratype, last-stage nauplius (LSN) from Sesoko Island photographed live before fixation (A) and in SEM after fixation and mounting (B–H). A, ventral view; B, ventral view; C, dorsal view; D, pair of ventral pores situated in front of labrum; E, close-up views of pores and sensilla of cephalic shield; F, caudal end in dorsal view; G, caudal end in ventral view; H, caudal end viewed from behind. Abbreviations: a1, first antenna; a2, second antenna; arthro membr, arthrodial membrane; ce, compound eye; dc sp, dorsocaudal spine; en, endopod; ex, exopod; fur sp, furcal spine; la, labrum; md, mandible; ne, nauplius eye; rud, rudimentary. Small Arabic numerals, many annotated with l or r for left and right, respectively, refer to cuticular structures (see Table 4). Yellow overlay, cephalic shield; blue overlay, faciotrunk; red overlay, anterior field of facets. Square with dotted outline: sample number, museum number and type status.

Figure 3 in Integrative taxonomy of crustacean y-larvae (Thecostraca: Facetotecta) using laboratory-rearing and molecular analyses of single specimens, with the description of a new vermiform species

Figure 3. Overview of most specimens of Hansenocaris demodex sp. nov. collected at Sesoko Island during fieldwork in 2018 and 2019. A, 19 specimens, four of which reached the cyprid stage, collected in the plankton at the naupliar stage and reared individually, with length, dish number, sample number, and museum registration number indicated for each specimen as well as a colour code (red, blue, yellow) showing its ultimate treatment/fixation (respectively, molecular work, SEM or exuvium on slide); some larvae died before preservation and are only referred to by their dish number; B, overview of setup for individual rearing of larvae; top view of green tray containing 31 2.5-cm-wide dishes with tracking information written on lids, each containing one to five live larvae. Live video of H. demodex and other y-larvae can be seen here: https:// youtu.be/seo-63AK10E.

Figure 1 in Integrative taxonomy of crustacean y-larvae (Thecostraca: Facetotecta) using laboratory-rearing and molecular analyses of single specimens, with the description of a new vermiform species

Figure 1. Facetotecta sampling sites in East Asia, 2017–20 and distribution of Hansenocaris demodex sp. nov. A, East China Sea showing distance (780 km) between the two main sampling sites, Sesoko Island (Okinawa, Japan) and Green Island (Taiwan). In 2020, several additional specimens of H. demodex were collected from Xiaoliuqiu Island (MJG, unpublished). B, Sesoko Island (Japan, Okinawa) with indication of sampling site (Sesoko Station); C, Green Island (Taiwan) with indication of sampling site (Gonguan Fishing Harbour); D, pier at Sesoko Station; E, Gonguan Fishing Harbour.

Figure 2 in Integrative taxonomy of crustacean y-larvae (Thecostraca: Facetotecta) using laboratory-rearing and molecular analyses of single specimens, with the description of a new vermiform species

Figure 2. Example of newly collected sample of still living y-larvae collected on 19 October 2018 at Sesoko Island off the pier shown in Figure 1D, representing a variety of developmental stages. A, 25 y-larvae likely representing> 15 different species, including 18 early lecithotrophic nauplii (*), five planktotrophic nauplii (†) and two cyprids (§). None of the depicted specimens can be assigned to already described species, but three of the planktotrophic nauplii are similar to Itô's (1986a) Pacific type I and one is a cyprid of the type nicknamed 'Big brown', both types of which are sequenced as a part of this work and belong to the same clade as Hansenocaris demodex sp. nov. (see Fig. 15). B, close-up of selected y-larvae with same numbers assigned as in A. Live video of H. demodex and other y-larvae can be seen here: https://youtu.be/seo-63AK10E.

Fig. 29 in Morphological and molecular evidence refute a broad circumscription for Pultenaea glabra (Fabaceae: Mirbelieae), with implications for taxonomy, biogeography, and conservation

Fig. 29. Pultenaea praecipua subsp. temperata (=P. sp. Nullo Mountain (M. A. M. Renner et al. 903)) photos. (a) Habit, showing the glaucous adaxial leaf surfaces and irregular branching. (b–f) Flowering shoots, showing buds produced in association with normal vegetative leaves. All from plants growing in the vicinity of NSW 1052799.

Fig. 26 in Morphological and molecular evidence refute a broad circumscription for Pultenaea glabra (Fabaceae: Mirbelieae), with implications for taxonomy, biogeography, and conservation

Fig. 26. Pultenaea praecipua subsp. praecipua (= P. sp. Genowlan Point (NSW 417813)) line drawing. (a) Flowering branch detail; (b) habit; (c) stipule; (d) flower, lateral view; (e) flower, perianth removed, lateral view; (f) standard petal, ventral view; (g) wing, lateral view; (h) keel, lateral view; (l) leaf abaxial surface; (j) leaf, adaxial surface; (k) leaf cross-section and adaxial surface; (l) leaf cross-section and abaxial surface. Scale bar: 10 mm (a); 26.7 mm (b); 2.7 mm (c); 8 mm (d–h); 5 mm (i, j), 1.6 mm (k, l). All from NSW 1052720, b from dried material, a, c–l from spirit.

Fig. 23 in Morphological and molecular evidence refute a broad circumscription for Pultenaea glabra (Fabaceae: Mirbelieae), with implications for taxonomy, biogeography, and conservation

Fig. 23. Pultenaea aculeata (= P. sp. Olinda (R. Coveny 6166)) line drawing. (a) Habit, (b) flowering branch detail, (c) flower, side view, (d) standard petal, (e) wing, (f) keel, (g) flower, side view, petals removed, (h) magnified calyx, side view, (i) ovary detail (j) stem detail showing stipule, (k) leaf, adaxial surface, (l) leaf, abaxial surface, (m) leaf surface detail, adaxial, (n) leaf surface detail, abaxial. Scale bar: 26.7 mm (a); 10 mm (b); 8 mm (c–g); 4 mm (h); 2.67 mm (i, j); 5 mm (k, l); 1.6 mm (m, n). All from NSW 1052810, a from dried material, b–n from spirit.

Fig. 20 in Morphological and molecular evidence refute a broad circumscription for Pultenaea glabra (Fabaceae: Mirbelieae), with implications for taxonomy, biogeography, and conservation

Fig. 20. Pultenaea mutabilis var. mutabilis (= P. sp. Lees Pinch (L. A. S. Johnson s.n.: NSW 17642)) photos. (a) A typical shoot, showing the flowers scattered among the leaves. (b–e) Close-ups of flowering shoot sectors, showing flowers produced in association with normal vegetative leaves, the green upper and lower leaf surfaces, the calyces that appear glabrous, but which bear short antrorse hairs, and the pale yellow keel. Note the short sparse hairs on the branchlets in e. All from plants at Mount Irvine, growing in the vicinity of NSW1058848.

Fig. 17 in Morphological and molecular evidence refute a broad circumscription for Pultenaea glabra (Fabaceae: Mirbelieae), with implications for taxonomy, biogeography, and conservation

Fig. 17. Pultenaea tenebrosa (= P. sp. Wolgan Cliffs (S. Clarke 602)) seedlings observed on 5 June 2021.

Fig. 16 in Morphological and molecular evidence refute a broad circumscription for Pultenaea glabra (Fabaceae: Mirbelieae), with implications for taxonomy, biogeography, and conservation

Fig. 16. Pultenaea tenebrosa (= P. sp. Wolgan Cliffs (S. Clarke 602)) photos. (a) Shoot apex showing flower in frontal view, note the splayed wings and nearly oblong standard which is taller than it is wide, in contrast to other members of the P. glabra complex; note also the strong glaucous bloom on the adaxial surface of the nearly terete leaves. (b) Close-up of flower buds showing the presence of sparse, long spreading hairs and the long, pungent bracteole, which extend as far as or beyond the calyx lobes; note also the production of normal or nearly normal leaves beneath each flower bud. (c) A flowering shoot, showing the flowers at the shoot apex in a close-set series, in which the internodes are of similar spacing to those separating adjacent vegetative leaves, note the leaves spreading perpendicular to the shoot below the flowers. (d) A group of flowers at the shoot apex, note the long pungent bracteoles, and the glaucous leaf adaxial surface, also note that the wings do not conceal the keel apex, in contrast to P. glabra. (e) Another view of flower buds. (f) Habit. (g) Close-up shoot showing mature stipules, which are characteristically charcoal–black and whose lobes are recurved, note again the leaves spreading perpendicular to the stem axis. All from plants growing in the vicinity of voucher NSW 1058842.

Fig. 15 in Morphological and molecular evidence refute a broad circumscription for Pultenaea glabra (Fabaceae: Mirbelieae), with implications for taxonomy, biogeography, and conservation

Fig. 15. Pultenaea tenebrosa (= P. sp. Wolgan Cliffs (S. Clarke 602)) line drawing. (a) Flowering branch detail; (b) habit; (c) stipule; (d) flower, lateral view; (e) flower, perianth removed, lateral view; (f) standard petal, ventral view; (g) wing, lateral view; (h) keel, lateral view; (l) leaf abaxial surface; (j) leaf, adaxial surface; (k) leaf cross section and adaxial surface; (l) leaf cross section and abaxial surface. Scale bar: 10 mm (a); 26.7 mm (b); c, 2.7 mm (c); 8 mm (d–h); 5 mm (i, j); 1.6 mm (k, l). All from NSW 1058844, b from dried material, a, c–l from spirit.