Find research datasets worth reusing

Fig. 1 in DNA barcoding reveals different cestode helminth species in northern European marine and freshwater ringed seals

Fig. 1. (A) Geographic distributions of the three northern European ringed seal subspecies from which cestodes were collected for COI barcoding: Baltic ringed seal (green), Saimaa ringed seal (blue), and Ladoga ringed seal (red). (B) Midpoint-rooted neighbor-joining tree based on K2P distances among COI barcode sequences of 35 cestode individuals collected from the three focal ringed seal subspecies. Individuals are colored according to host subspecies, numbers above or next to branches are bootstrap support values based on 500 resamplings of the data matrix (only values> 70% shown). Cestode species names indicated under the main branches are based on barcode similarity to reference sequences in GenBank. (C) Maximum-likelihood tree based on a 562-bp alignment of the barcode sequences of the focal cestodes and 34 diphyllobothriidean reference taxa obtained from GenBank. Numbers above branches are bootstrap support values based on 100 resamplings of the data (only values> 70% shown). In both trees, individual names include the voucher code or GenBank accession number, seal subspecies abbreviation with seal individual code, barcode-based cestode species name, and name of the host (sub)species from which the cestode specimen was collected. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Figs 11- 18 in Crane flies (Diptera, Tipuloidea) from southern Neotropical salt marshes: survey with DNA barcoding

Figs 11- 18. Morphological characteres for Tipuloidea genera identification. (11) Gonomyia (Neolipophleps), (12) Gonomyia (Paralipophleps), (13) Molophilus and (14) Ormosia, wings; (15) Rhipidia and (16) Dicranomyia, antennae; (17) Symplecta (Symplecta) and (18) Symplecta (Trimicra), wings. Modified from GELHAUS (2009).

Fig. 2 in Crane flies (Diptera, Tipuloidea) from southern Neotropical salt marshes: survey with DNA barcoding

Fig. 2. DNA barcoding gap analysis, with frequency of intra and interspecific distances in COI sequences among Tipuloidea species.

Figs 3-10 in Crane flies (Diptera, Tipuloidea) from southern Neotropical salt marshes: survey with DNA barcoding

Figs 3-10. Morphological characteres for Tipuloidea genera identification: (3) Tipulidae*, (4) Limoniidae, head; (5) Ozodicera*, antennae. (6) Nephrotoma* and (7) Zelandotipula, wings; (8) Toxorhina*, (9) Geranomyia* and (10) Teucholabis*, head (Sc, Subcostal vein; Rs, Radial sector vein; bm-cu, Basalmedial cubital vein; dm, Discal-medial cell). *Modified from GELHAUS (2009).

Fig. 1 in Crane flies (Diptera, Tipuloidea) from southern Neotropical salt marshes: survey with DNA barcoding

Fig. 1. Sampling areas at salt marshes of the Patos Lagoon Estuary, Rio Grande do Sul, southern Brazil.

FIGURE 4 in Integrative taxonomy reveals disjunct distribution and first record of Hoplias misionera (Characiformes: Erythrinidae) in the Amazon River basin: morphological, DNA barcoding and cytogenetic considerations

FIGURE 4 | Neighbor joining (NJ) tree of Hoplias inferred from partial COI (Cytochrome c Oxidase Subunit I gene) sequences using the Kimura 2-parameter model. The lateral bar indicates the partitions of species delimitation performed by the GMYC, ABGD and BIN analysis. The clade Hoplias misionera nested individuals from the La Plata and Amazon basins (blue tips).

FIGURE 7 in Integrative taxonomy reveals disjunct distribution and first record of Hoplias misionera (Characiformes: Erythrinidae) in the Amazon River basin: morphological, DNA barcoding and cytogenetic considerations

FIGURE 7 | Updated distribution map of Hoplias misionera showing former known localities in Argentina and southern Brazil (Rosso et al., 2016) and the new records from Amazon basin (triangles). Star = type locality.

FIGURE 1 in Integrative taxonomy reveals disjunct distribution and first record of Hoplias misionera (Characiformes: Erythrinidae) in the Amazon River basin: morphological, DNA barcoding and cytogenetic considerations

FIGURE 1 | Hoplias misionera, UFOPA AMTRA131-19, 237 mm SL, Amazonas River, Alenquer, Pará, Brazil. Lateral view. Scale bar = 1 cm. Photo by L.R.R. Rodrigues.

FIGURE 2 in Integrative taxonomy reveals disjunct distribution and first record of Hoplias misionera (Characiformes: Erythrinidae) in the Amazon River basin: morphological, DNA barcoding and cytogenetic considerations

FIGURE 2 | Configuration of the medial margins of the dentary in Hoplias misionera. A. Y-shaped, UFOPA AMTRA126-19, 214 mm SL. B. V-shaped, UFOPA AMTRA127-19, 232 mm SL. Scale bars = 1 cm. Photos by L. R. R. Rodrigues. Illustration by T. M. A. Lima.

FIGURE 6 in Integrative taxonomy reveals disjunct distribution and first record of Hoplias misionera (Characiformes: Erythrinidae) in the Amazon River basin: morphological, DNA barcoding and cytogenetic considerations

FIGURE 6 | Partial idiogram of the four largest chromosome pairs of Hoplias malabaricus (karyomorphs C and F) and H. misionera showing marked size reduction from the first to second metacentric pair only in the karyomorph F.

FIGURE 3 in Integrative taxonomy reveals disjunct distribution and first record of Hoplias misionera (Characiformes: Erythrinidae) in the Amazon River basin: morphological, DNA barcoding and cytogenetic considerations

FIGURE 3 | Last vertical series of scales on the base of the caudal-fin rays. Comparison between Hoplias misionera (A), UFOPA AMTRA131-19, 237 mm SL and Hoplias cf. malabaricus (B), UFOPA AMTRA110, 201 mm SL. Scale bars = 1 cm. Photos by L. R. R. Rodrigues. Illustration by T. M. A. Lima.

FIGURE 5 in Integrative taxonomy reveals disjunct distribution and first record of Hoplias misionera (Characiformes: Erythrinidae) in the Amazon River basin: morphological, DNA barcoding and cytogenetic considerations

FIGURE 5 | Karyotype of Hoplias misionera from Amazon basin (2n=40 chromosomes). Conventional Giemsa stained (A), C-banded (B) and mapping of 18S rDNA FISH probes (green signals) (C). The Ag-NOR bearing chromosomes are showed in the box.

FIGURE 3 in DNA Barcoding of Pyrrhulina australis (Characiformes: Lebiasinidae) reveals unexpected cryptic diversity in the group

FIGURE 3 | Dendrogram of the Pyrrhulina species based on a Bayesisan Inference analysis of the COI sequences obtained in the present study. The red bars represent the consensus MOTUs, defined according to the congruity between the results of the species delimitation methods applied in the present study. The black bars represent the Molecular Operational Units (MOTUs) formed by the different species delimitation methods: Optimal Threshold (OT); Assemble Species by Automatic Partitioning (ASAP); Poisson Tree Processes (PTP) and Generalized Mixed Yule Coalescence (GMYC). Bars marked with a star represent the same MOTU under the OT analysis. The sequence codes in bold script indicate the samples obtained from the BOLD systems database.

FIGURE 2 in DNA Barcoding of Pyrrhulina australis (Characiformes: Lebiasinidae) reveals unexpected cryptic diversity in the group

FIGURE 2 | Morphospecies considered in this study: A. Pyrrhulina aff. australis I (Araguaia, modified from Venere, Garuti, 2011); B. P. obermulleri (Madeira); C. P. aff. australis IV (Guaporé); D. P. marilynae (Teles Pires) and E. P. australis (Pantanal, Paraguay basin). Pyrrhulina spilota and P. filamentosa are not shown here because the sequences were obtained from the BOLD systems database, and no physical specimens were collected in the field.

FIGURE 1 in DNA Barcoding of Pyrrhulina australis (Characiformes: Lebiasinidae) reveals unexpected cryptic diversity in the group

FIGURE 1 | Geographic distribution of the Pyrrhulina morphospecies and MOTUs throughout South American river basins.

FIGURE 5 in DNA Barcoding of Pyrrhulina australis (Characiformes: Lebiasinidae) reveals unexpected cryptic diversity in the group

FIGURE 5 | Quadrant plot showing the maximum K2P intraspecific distances and the maximum K2P interspecific in percentages for the MOTUs of Pyrrhulina identified in the present study. The lines indicate the threshold (1.79%) between the intra- and interspecific distances. The morphology of the species in quadrant I is consistent with the molecular identification. The species in quadrant II probably have cryptic forms. Species present in quadrant III are likely the result of recent divergence, hybridization or synonimization, while quadrant IV represents a lack of correspondence between the morphological and molecular identifications.

FIGURE 4 in DNA Barcoding of Pyrrhulina australis (Characiformes: Lebiasinidae) reveals unexpected cryptic diversity in the group

FIGURE 4 | Quadrant plot showing the maximum K2P intraspecific distances and the maximum K2P interspecific in percentages for the nominal Pyrrhulina species analyzed in the present study. The lines indicate the threshold (1.79%) between the intra- and interspecific distances. The morphology of the species in quadrant I is consistent with the molecular identification. The species in quadrant II probably have cryptic forms. Species present in quadrant III are likely the result of recent divergence, hybridization or synonimization, while quadrant IV represents a lack of correspondence between the morphological and molecular identifications.

Fig. 5 in Same information, new applications: revisiting primers for the avian COI gene and improving DNA barcoding identification

Fig. 5 Percentage of template sequences coverage of the primer subsets analyzed on the second evaluation round. The red dashed line represents the complete coverage of the analyzed template sequences

Fig. 4 Primers binding position distributed along the 1,500 in Same information, new applications: revisiting primers for the avian COI gene and improving DNA barcoding identification

Fig. 4 Primers binding position distributed along the 1,500 bp of the avian COI gene. A Binding position of forward primers. B Binding position of reverse primers

Fig. 3 in Same information, new applications: revisiting primers for the avian COI gene and improving DNA barcoding identification

Fig. 3 Effect of the number of allowed primer-template mismatches on primer binding. Y-axis = number of primers with at least one binding event on every scenario of allowed mismatches (X-axis)