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Supplementary material 4 from: Malek-Hosseini MJ, Jugovic J, Fatemi Y, Kuntner M, Kostanjšek R, Douady CJ, Malard F (2022) A new obligate groundwater species of Asellus (Isopoda, Asellidae) from Iran. Subterranean Biology 42: 97-124. https://doi.org/10.3897/subtbiol.42.79447
Table S4. COI alignment
Supplementary material 6 from: Malek-Hosseini MJ, Jugovic J, Fatemi Y, Kuntner M, Kostanjšek R, Douady CJ, Malard F (2022) A new obligate groundwater species of Asellus (Isopoda, Asellidae) from Iran. Subterranean Biology 42: 97-124. https://doi.org/10.3897/subtbiol.42.79447
Table S6. Morphological comparison with other Asellus species
Supplementary material 7 from: Malek-Hosseini MJ, Jugovic J, Fatemi Y, Kuntner M, Kostanjšek R, Douady CJ, Malard F (2022) A new obligate groundwater species of Asellus (Isopoda, Asellidae) from Iran. Subterranean Biology 42: 97-124. https://doi.org/10.3897/subtbiol.42.79447
Table S7. Genetic distances among and within MOTUs
Figure 4 from: Malek-Hosseini MJ, Jugovic J, Fatemi Y, Kuntner M, Kostanjšek R, Douady CJ, Malard F (2022) A new obligate groundwater species of Asellus (Isopoda, Asellidae) from Iran. Subterranean Biology 42: 97-124. https://doi.org/10.3897/subtbiol.42.79447
Figure 4 Asellus ismailsezarii sp. nov., Ganow spring, Western Iran. Paratype male, 8.1 mm (TU-SP. 2), mouthparts: MdL, left mandible; Mx I, maxilla I; Mx II, maxilla II, MxlpR (v), right maxilliped (ventrally).
Figure 6 from: Malek-Hosseini MJ, Jugovic J, Fatemi Y, Kuntner M, Kostanjšek R, Douady CJ, Malard F (2022) A new obligate groundwater species of Asellus (Isopoda, Asellidae) from Iran. Subterranean Biology 42: 97-124. https://doi.org/10.3897/subtbiol.42.79447
Figure 6 Asellus ismailsezarii sp. nov., Ganow spring, Western Iran. Holotype male, 8.2 mm: Plp I, II; II, IV, V – pleopods I, II, III, IV, IV; U – uropod (pleopod VI). Female, 8.1 mm: Plp II (f) – pleopod II. Juvenile male, 7.3 mm: Plp I, II (juv) – pleopods I, II.
Figure 3 from: Malek-Hosseini MJ, Jugovic J, Fatemi Y, Kuntner M, Kostanjšek R, Douady CJ, Malard F (2022) A new obligate groundwater species of Asellus (Isopoda, Asellidae) from Iran. Subterranean Biology 42: 97-124. https://doi.org/10.3897/subtbiol.42.79447
Figure 3 Asellus ismailsezarii sp. nov., Ganow spring, Western Iran. Holotype male, 8.2 mm: A I, II antennae I and II B body dorsal view.
Figure 7 from: Malek-Hosseini MJ, Jugovic J, Fatemi Y, Kuntner M, Kostanjšek R, Douady CJ, Malard F (2022) A new obligate groundwater species of Asellus (Isopoda, Asellidae) from Iran. Subterranean Biology 42: 97-124. https://doi.org/10.3897/subtbiol.42.79447
Figure 7 Asellus ismailsezarii sp. nov., male; SEM micrographs of (A) pleopod I (paratype male BL 8.2 mm: TU-SP.2). Photo also shows one genital papilla inserted on ventral side of pereionite VIII B, C, D pleopod II endopod (holotype male BL 8.2 mm: TU-SP.1) B, C ventral view and D lateral view.
Figure 2 from: Malek-Hosseini MJ, Jugovic J, Fatemi Y, Kuntner M, Kostanjšek R, Douady CJ, Malard F (2022) A new obligate groundwater species of Asellus (Isopoda, Asellidae) from Iran. Subterranean Biology 42: 97-124. https://doi.org/10.3897/subtbiol.42.79447
Figure 2 Habitus of Asellus ismailsezarii sp. nov. (Female AS1). The photo shows a specimen exposed to air after the stone it was under had been turned over. Body length is 8.6 mm.
Figure 9 from: Malek-Hosseini MJ, Jugovic J, Fatemi Y, Kuntner M, Kostanjšek R, Douady CJ, Malard F (2022) A new obligate groundwater species of Asellus (Isopoda, Asellidae) from Iran. Subterranean Biology 42: 97-124. https://doi.org/10.3897/subtbiol.42.79447
Figure 9 Map showing the distribution of molecular operational taxonomic units (MOTU) within the "Asellus pattern". Site locations for Asellus hilgendorfii (Japan) and Mesoasellus dybowskii and Limnoasellus poberezhnii (Lake Baikal, Russia) are not shown. Colors and symbols as in Fig. 8.
Figure 8 from: Malek-Hosseini MJ, Jugovic J, Fatemi Y, Kuntner M, Kostanjšek R, Douady CJ, Malard F (2022) A new obligate groundwater species of Asellus (Isopoda, Asellidae) from Iran. Subterranean Biology 42: 97-124. https://doi.org/10.3897/subtbiol.42.79447
Figure 8 Phylogenetic relationships among taxa belonging to the Asellus pattern derived from Maximum likelihood analysis of (A) 585 COI mtDNA sequences, (B) thirty 28S rDNA sequences. The clade comprised of M. dybowskii and L. poberezhnii was used as outgroup. In A, branches to molecular operational taxonomic units (MOTU) as delimited with the fixed threshold method implemented by Lefébure et al. (2006) were collapsed. In B, branches to the main clades were collapsed. Colors and symbols as in Fig. 9. Branch supports are approximate likelihood ratio test (aLRT) values (black) and bootstrap values (red).
Figure 1 from: Malek-Hosseini MJ, Jugovic J, Fatemi Y, Kuntner M, Kostanjšek R, Douady CJ, Malard F (2022) A new obligate groundwater species of Asellus (Isopoda, Asellidae) from Iran. Subterranean Biology 42: 97-124. https://doi.org/10.3897/subtbiol.42.79447
Figure 1 Photo of Ganow spring, the type locality of Asellus ismailsezarii sp. nov., Tuveh village, Andimeshk, Khuzestan Province, Iran. Stream width is about one meter.
Supplementary material 2 from: Malek-Hosseini MJ, Jugovic J, Fatemi Y, Kuntner M, Kostanjšek R, Douady CJ, Malard F (2022) A new obligate groundwater species of Asellus (Isopoda, Asellidae) from Iran. Subterranean Biology 42: 97-124. https://doi.org/10.3897/subtbiol.42.79447
Table S2. List of primers
Supplementary material 3 from: Malek-Hosseini MJ, Jugovic J, Fatemi Y, Kuntner M, Kostanjšek R, Douady CJ, Malard F (2022) A new obligate groundwater species of Asellus (Isopoda, Asellidae) from Iran. Subterranean Biology 42: 97-124. https://doi.org/10.3897/subtbiol.42.79447
Table S3. COI and 28S sequence data set with sampling localities
Supplementary material 5 from: Malek-Hosseini MJ, Jugovic J, Fatemi Y, Kuntner M, Kostanjšek R, Douady CJ, Malard F (2022) A new obligate groundwater species of Asellus (Isopoda, Asellidae) from Iran. Subterranean Biology 42: 97-124. https://doi.org/10.3897/subtbiol.42.79447
Table S5. 28S alignment
Supplementary material 1 from: Malek-Hosseini MJ, Jugovic J, Fatemi Y, Kuntner M, Kostanjšek R, Douady CJ, Malard F (2022) A new obligate groundwater species of Asellus (Isopoda, Asellidae) from Iran. Subterranean Biology 42: 97-124. https://doi.org/10.3897/subtbiol.42.79447
Table S1. DNA extraction and PCR protocols
Figure 5 from: Malek-Hosseini MJ, Jugovic J, Fatemi Y, Kuntner M, Kostanjšek R, Douady CJ, Malard F (2022) A new obligate groundwater species of Asellus (Isopoda, Asellidae) from Iran. Subterranean Biology 42: 97-124. https://doi.org/10.3897/subtbiol.42.79447
Figure 5 Asellus ismailsezarii sp. nov., Ganow spring, Western Iran. Holotype male, 8.2 mm: Pp I, IV, VII – pereopods I (distal articles), IV, VII. Female, 8.1 mm: Pp I, IV (f) – distal articles of pereopods I, IV.
Supplementary material 2 from: Dooley KE, Niemiller KDK, Sturm N, Niemiller ML (2022) Rediscovery and phylogenetic analysis of the Shelta Cave Crayfish (Orconectes sheltae Cooper & Cooper, 1997), a decapod (Decapoda, Cambaridae) endemic to Shelta Cave in northern Alabama, USA. Subterranean Biology 43: 11-31. https://doi.org/10.3897/subtbiol.43.79993
Table S2
Supplementary material 1 from: Dooley KE, Niemiller KDK, Sturm N, Niemiller ML (2022) Rediscovery and phylogenetic analysis of the Shelta Cave Crayfish (Orconectes sheltae Cooper & Cooper, 1997), a decapod (Decapoda, Cambaridae) endemic to Shelta Cave in northern Alabama, USA. Subterranean Biology 43: 11-31. https://doi.org/10.3897/subtbiol.43.79993
Table S1
Supplementary material 1 from: Rossetti G, Stoch F, Mazzini I (2022) A reassessment of the origin and distribution of the subterranean genus Pseudolimnocythere Klie, 1938 (Ostracoda, Loxoconchidae), with description of two new species from Italy. Subterranean Biology 43: 33-60. https://doi.org/10.3897/subtbiol.43.82158
Figures S1–S5
Supplementary material 1 from: Premate E, Fišer Ž, Kuralt Ž, Pekolj A, Trajbarič T, Milavc E, Hanc Ž, Kostanjšek R (2022) Behavioral observations of the olm (Proteus anguinus) in a karst spring via direct observations and camera trapping. Subterranean Biology 44: 69-83. https://doi.org/10.3897/subtbiol.44.87295
Figure S1
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.