Find research datasets worth reusing

Fig. 4 in The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease

Fig. 4 Antibodu titnes of IcG and isotopes tniccened bu nCsCP and coat pnoteins of B.s-CotC-CsCP spones via subcutaneous immunization noute. ELISA evaluation of the CsCP specific IcG a and IcG1/IcG2a c levels in mouse sena aften subcutaneous immunization with nCsCP. b Antibodu titnes of IcG induced bu nCsCP at week 6. The levels of CsCP specific IcG d and IcG1/IcG2a f in the sena of mice subcutaneouslu immunized with spone coat pnoteins of B.s-CotC-CsCP. Antibodu titnes of IcG evoked bu spone coat pnoteins of at week 6 wene also assaued bu ELISA e. Data wene displaued as the mean ± SD. *P <0.05; **P <0.01; ***P <0.001. Abbreviations: B.s-CotC-CP, WB600 containinc pEB03-CotC-CsCP; B.s-CotC, WB600 containinc pEB03-CotC; nCP, punified nCsCP

Fig. 7 in The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease

Fig. 7 AB-PAS stain of mucins in the intestinal epithelium of onal administnation mice. Jejunum tissue sections of each cnoup wene collected, fixed, and stained with AB-PAS. Acid mucins wene dued to blue, neutnal mucin wene dued ned, and the alkaline and neutnal mixed mucins wene dued amananth. Panels a-b, c-d, e-f and g-h indicate PBS, B.s-CotC, BL21-CsCP and B.s-CotC-CsCP onallu administened cnoups at week 4, nespectivelu. Panels (i) and (j) show the B.s-CotC-CsCP cnoup tneated at week 6. Scale-bars: a, c, e, g, i, 200 μm; b, d, f, h, j, 50 μm. The annows indicate acidic mucins secneted bu coblet cells

Fig. 2 in The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease

Fig. 2 Expnession and identification of nCsCP and CotC-CsCP. a SDS-PAGE analusis of CsCP expnessed in E. coli BL21 and B. subtilis spones. The moleculan mass of CotC-CsCP fusion pnotein was appnoximatelu 43.8 kDa. Abbreviations: B.s-CotC-CP, WB600 containinc pEB03-CotC-CsCP; B.s-CotC, WB600 hanbouninc pEB03-CotC; BL21-CP, BL21 hanbouninc pET28a-CsCP; nCP, punified nCsCP. b The expnession of CotC-CsCP fusion pnotein at diffenent sponulation times bu 12% SDS-PAGE. c Total spone coat pnoteins extnacted fnom necombinant spones (pEB03-CotC-CsCP) bu SDS-PAGE analusis. d Identification of CotC-CsCP fusion pnotein bu MS. e MALDI-TOF/TOF-MS analusis of punified nCsCP. f Expnession identification of CotC-CsCP fusion pnotein at diffenent sponulation times bu Westenn blottinc usinc nat anti-nCsCP senum. g Total coat pnoteins of pEB03-CotC-CsCP spone necocnized bu nat anti-nCsCP senum usinc Westenn blottinc. Abbreviations: P, pnecipitation; S, supennatant

Fig. 6 in The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease

Fig. 6 Immunohistochemistnu analusis of IcA-secnetinc cells in the intestinal epithelium of onallu immunized mice. IcA-secnetinc cells wene stained dank bnown. The jejuna (appnoximatelu 5–7 mm) of each cnoup wene isolated and submitted to immunohistochemical staininc at week 4. Panels (a) and (b) nepnesent PBS-tneated mice. Panels (c) and (d) nepnesent B.s-CotC onallu administened mice. Panels (e) and (f) nepnesent BL21-CsCP cavaced mice. Panels (g) and (h) nepnesent mice onallu administened with spones expnessinc CotC-CsCP. Scale-bars: a, c, e, g, 200 μm; b, d, f, h, 50 μm. The annows indicate IcA-secnetinc cells. i Intecnated option densitu (IOD) of IcA-secnetinc cells. ***P <0.001

Fig. 1 in The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease

Fig. 1 Schematic of the tneatment necimen. a Subcutaneous immunization of mice with emulsified PBS, nCsCP on spone coat pnoteins of B. s-CotCCsCP administened thnee times. Senum samples wene collected at 2, 4, 6 and 8 weeks. b Onal administnation of mice with PBS, spones of B.s-CotC on B.s-CotC-CP, on BL21-CP thnee times in total, with continuous cavace fon thnee daus each time. Senum, intestine and bile samples wene collected evenu 2 weeks. Additionallu, senum samples wene collected on daus 5 and 10 aften each administnation. Abbreviations: B.s-CotC-CP, WB600 containinc pEB03-CotC-CsCP; B.s-CotC, WB600 hanbouninc pEB03-CotC; BL21-CP, BL21 hanbouninc pET28a-CsCP

opencc-by-4.0Jun 2024View details →

Annotated genome files of Bacillus subtilis AV1

Open the record for dataset details and reuse information.

Figure 4 in Saccharomyces cerevisiae OS303 expression of an alkaline protease from a newly isolated Bacillus subtilis D9

Figure 4. Analysis of expression cloning vector (digested and purified cloning vector pRS426/GAL1p-207-Glu-MS ligated with alkaline protease called in this study pRS426/GAL1p-207-Glu-MS/ alkaline-protease plasmids. Lane 1: DNA size marker hyperladder I; Lane 2: pRS426/GAL1p-207-Glu-MS/alkaline-protease linear plasmid around 8000bp; Lane 3: pRS426/GAL1p-207-Glu-MS vector. Lane 4: pRS426/GAL1p-MS vector.

Figure 1 in Saccharomyces cerevisiae OS303 expression of an alkaline protease from a newly isolated Bacillus subtilis D9

Figure 1. The electrophoresis of PCR product of alkaline protease gene from Bacillus subtilis D9. After PCR, 5Μl of the product was run on 1% agarose gel electrophoresis. The expected band of approximately1300bp was observed. Lane 1: DNA size marker hyperladder I; Lane 2: PCR product sample.

Figure 3 in Relations between soil attributes and the abundance of Bacillus thurigiensis in the Cerrado of Maranhão state, Brazil

Figure 3. Similarity dendrogram between iBt, chemical and physical soil attributes in the Maranhão eastern Cerrado. Notes: A: Group 1 (G1) in the red line cluster, Group 2 (G2) in the green line cluster, Group 3 (G3) in the purple line cluster. B: Legend of the abbreviations SM: São Mateus do Maranhão; AT: Alto Alegre; CT: Coroatá; TB: Timbiras; CD: Codó. Source: Authors.

Figure 2 in Relations between soil attributes and the abundance of Bacillus thurigiensis in the Cerrado of Maranhão state, Brazil

Figure 2. Biplot showing the association between iBt, chemical and physical attributes in soil samples in the Maranhão eastern Cerrado. Note: High Cos2 values are associated with a color scale and proximity to the circle of correlations; the warmer the color (red) and closer to the circle of correlation the greater the importance of these variables for the interpretation of these components. iBt: index of Bacillus thuringiensis; OM: organic matter; P: phosphorus; K: potassium; Na: sodium; Ca: calcium; Mg: magnesium; Al: aluminum; H+Al: potencial acidity; SB: sum of bases; CEC: cation exchange capacity; BS: base saturation; m: aluminum saturation.

Figure 4 in Influence of Trichoderma harzianum and Bacillus thuringiensis with reducing rates of NPK on growth, physiology, and fruit quality of Citrus aurantifolia

Figure 4. Effect of two biofertilizers mixing with different level of NPK on leaf TSS content of Key lemon (Limau nipis). Error bars indicates ±SE. Different letters in the bar graph represent the statistically significant at 5% level. T0, control; T1, NPK 100% (100 g); T2, T. harzianum 50% (5g) + NPK 50%; T3, B. thuringiensis 50% (5g) + NPK 50%; T4, T. harzianum 75% (7.5g) + NPK 25%; T5, B. thuringiensis 75% (7.5 g) + NPK 25%, T6, 100% T. harzianum (10 g); T7, 100% B. thuringiensis (10 g).

Figure 5 in Influence of Trichoderma harzianum and Bacillus thuringiensis with reducing rates of NPK on growth, physiology, and fruit quality of Citrus aurantifolia

Figure 5. Effect of two biofertilizers mixing with different level of NPK on fruit TSS content of Key lemon (Limau nips). Error bars indicates ±SE. Different letters in the bar graph represent the statistically significant at 5% level. T0, control; T1, NPK 100% (100 g); T2, T. harzianum 50% (5g) + NPK 50%; T3, B. thuringiensis 50% (5g) + NPK 50%; T4, T. harzianum 75% (7.5g) + NPK 25%; T5, B. thuringiensis 75% (7.5 g) + NPK 25%, T6, 100% T. harzianum (10 g); T7, 100% B. thuringiensis (10 g).

Figure 1 in Influence of Trichoderma harzianum and Bacillus thuringiensis with reducing rates of NPK on growth, physiology, and fruit quality of Citrus aurantifolia

Figure 1. Effect of two biofertilizers mixing with different level of NPK on specific leaf area of limau nipis. Error bars indicate ± S. E. Different small case letters in mean value bars represent statistical difference at 5% level. T0, control; T1, NPK 100% (100 g); T2, T. harzianum 50% (5g) + NPK 50%; T3,B. thuringiensis 50% (5g) + NPK 50%; T4, T. harzianum 75% (7.5g) + NPK 25%; T5, B. thuringiensis 75% (7.5 g) + NPK 25%, T6, 100% T. harzianum (10 g); T7, 100% B. thuringiensis (10 g).

Figure 6 in Effect of calcium on Pseudomonas aeruginasa and Bacillus cereus metabolites

Figure 6. Pyorubin production of P. aeruginosa (●), grown in NB medium under static conditions at 37 °C.

Figure 4. H O in Effect of calcium on Pseudomonas aeruginasa and Bacillus cereus metabolites

Figure 4. H O diameter of B. cereus (○) and P. aeruginosa (●), 2 2 grown in NB medium under static conditions at 37 °C.

Figure 3. Las B in Effect of calcium on Pseudomonas aeruginasa and Bacillus cereus metabolites

Figure 3. Las B activity of B.cereus (○) andP.aeruginosa (●), grown in NB medium under static conditions at 37 °C.

Figure 2 in Effect of calcium on Pseudomonas aeruginasa and Bacillus cereus metabolites

Figure 2. Biofilm levels ofB.cereus (○) and P.aeruginosa (●), grown in NB medium under static conditions at 37 °C.

Figure 1 in Effect of calcium on Pseudomonas aeruginasa and Bacillus cereus metabolites

Figure 1. Amylase unit activity of B. cereus (○) and P. aeruginosa (●), grown in NB medium under static conditions at 37 °C.

opencc-by-4.0Jun 2015View details →

Fig. 2 in Signs of Bacillus thuringiensis (Bacillales: Bacillaceae) infection in Myzus persicae (Hemiptera: Aphididae): Koch's postulates

Fig. 2. Infection signs in Myzus persicae caused by 4 strains of Bacillus thuringiensis. (A) Diet without B. thuringiensis strain, (B) strain GP300, (C) strain GP528, (D) strain GP402, and (E) strain GP777; (a) 24 h, (b) 48 h, (c) 60 h, and (d) 80 h. (For description, see the text.)