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64 results for “Botryosphaeriaceae”
FIGURE 13 in Two new species of Botryosphaeriaceae (Botryosphaeriales) and new host/ geographical records
FIGURE 13. Neodeightonia phoenicum on dead leaf base of Phoenix reclinata (MFLU 22-0096). a, b. Host. c, d. Appearance of conidiomata on host surface. e. Section through conidiomata. f. Section through peridium. g–j. Conidiogenous cells. k−n. Conidia. o. Germinating conidium. p, q. Colony on PDA (p upper, q lower). Scale bars: c = 2 mm, d = 100 μm, e = 50 μm, f, g = 5 μm, h–o = 10 μm.
FIGURE 14 in Two new species of Botryosphaeriaceae (Botryosphaeriales) and new host/ geographical records
FIGURE 14. Botryosphaeria fabicerciana on dead twig of Prunus serrulate (NCYUCC 19-0405). a, b. Appearance of ascostromata on host surface. c. Section through ascoma. d. Peridium. e. Pseudoparaphyses. f–h. Asci. i–l. Ascospores. m. Germinating ascospore. n, o. Colony on PDA (n upper, o lower). Scale bars: a = 500 μm, b, f–h = 50 μm, c = 100 μm, d, e, m = 20 μm, i–l = 10 μm.
FIGURE 2 in Two new species of Botryosphaeriaceae (Botryosphaeriales) and new host/ geographical records
FIGURE 2. Phylogram generated from ML analysis based on combined dataset of ITS, tef1-α and β-tub. The tree is rooted to Barriopsis stevensiana (CBS 174.26) and B. tectonae (MFLUCC 12-0381). Tree topology is similar to previous study conducted by Dissanayake et al. (2021). Bootstrap support values for ML ≥ 70% and Bayesian posterior probabilities (PP) ≥ 0.90 are noted at the nodes. Strain numbers are noted after the species names. Strains isolated in this study are presented in red and type strains are in bold.
FIGURE 5 in Two new species of Botryosphaeriaceae (Botryosphaeriales) and new host/ geographical records
FIGURE 5. Dothiorella obovata on dead twig of Pavonia odorata (holotype: MFLU 22-0094). a, b. Appearance of ascostromata on host surface. c, d. Section through ascomata. e. Section through peridium. f. Pseudoparaphyses. g–j. Asci. k–n. Ascospores. O. Germinating ascospore. p, q. Colony on PDA (p upper, q lower). Scale bars: a = 1 mm, b = 500 μm, c, d =100 μm, e, f = 10 μm, g–j = 20 μm, k–o = 5 μm.
FIGURE 6 in Two new species of Botryosphaeriaceae (Botryosphaeriales) and new host/ geographical records
FIGURE 6. Dothiorella albiziae on dead dry pod of Albizia lebbeck (holotype: MFLU 22-0093). a. Host. b, c. Conidiomata on dry pod surface. d. Vertical section through conidioma. e. Peridium of conidioma. f–i. Conidia attached to conidiogenous cells. j–m. Conidia. n, o. Colony on PDA (n upper, o lower). Scale bars: b = 2 mm, c = 500 μm, d = 100 μm, e–m = 10 μm.
FIGURE 4 in Two new species of Botryosphaeriaceae (Botryosphaeriales) and new host/ geographical records
FIGURE 4. Phylogram generated from ML analysis based on combined dataset of ITS, tef1-α and β-tub. The tree is rooted to Macrophomina phaseolina (CBS 227.33 and CBS 162.25). Tree topology is similar to previous study conducted by Rathnayaka et al. (2021). Bootstrap support values for ML ≥ 75% and Bayesian posterior probabilities (PP) ≥ 0.90 are noted at the nodes. Strain numbers are noted after the species names. The strain isolated in this study is presented in red and type strains are in bold.
FIGURE 10 in Two new species of Botryosphaeriaceae (Botryosphaeriales) and new host/ geographical records
FIGURE 10. Sphaeropsis eucalypticola on dead branch of Tamarindus indica (MFLU 22-0095). a, b. Appearance of ascostromata on host surface. c, d. Sections through ascomata. e. Section through peridium. f. Pseudoparaphyses. g–j. Asci. k–p. Ascospores. Scale bars: a = 2 mm, b, c = 200 μm, d = 100 μm, e, f = 20 μm, g–j = 50 μm, k–p = 10 μm.
FIGURE 3 in Two new species of Botryosphaeriaceae (Botryosphaeriales) and new host/ geographical records
FIGURE 3. Phylogram generated from ML analysis based on combined dataset of ITS, LSU, SSU, and tef1-α. The tree is rooted to Diplodia mutila (CBS 112553) and D. rosulata (CBS 116470). Tree topology is similar to previous study conducted by Phukhamsakda et al. (2022). Bootstrap support values for ML ≥ 70% and Bayesian posterior probabilities (PP) ≥ 0.90 are noted at the nodes. Strain numbers are noted after the species names. Strains isolated in this study are presented in red and type strains are in bold.
FIGURE 1 in Two new species of Botryosphaeriaceae (Botryosphaeriales) and new host/ geographical records
FIGURE 1. Phylogram generated from ML analysis based on combined dataset of ITS, LSU, tef1-α and β-tub. The tree is rooted to Neofusicoccum luteum (CMW 4165 and CBS 562.26). Tree topology is similar to the previous study by Zhang et al. (2021). Bootstrap support values for ML ≥ 70% and Bayesian posterior probabilities (PP) ≥ 0.90 are noted at the nodes. Strain numbers are noted after the species names. Strains isolated in this study are presented in red and type strains are in bold.
FIGURE. Dothiorella viticola on dead branch of Morus sp. (MFLU 19-0621). a, b. Conidiomata on host substrate. c. Vertical section through conidioma. d. Ostiole. e. Peridium of conidioma. f–j. Conidia attached to conidiogenous cells. k–m. Conidia. n. Germinating conidium. o, p. Colony on PDA (o upper, p lower). Scale bars: a = 1 mm, b = 100 μm, c = 50 μm, d = 20 μm, e–n = 10 μm. in Two new species of Botryosphaeriaceae (Botryosphaeriales) and new host/ geographical records
FIGURE. Dothiorella viticola on dead branch of Morus sp. (MFLU 19-0621). a, b. Conidiomata on host substrate. c. Vertical section through conidioma. d. Ostiole. e. Peridium of conidioma. f–j. Conidia attached to conidiogenous cells. k–m. Conidia. n. Germinating conidium. o, p. Colony on PDA (o upper, p lower). Scale bars: a = 1 mm, b = 100 μm, c = 50 μm, d = 20 μm, e–n = 10 μm.
FIGURE. Sphaeropsis linhaiensis on dead branch of Cinnamomum camphora (HKAS 10-2383). a, b. Appearance of ascostromata on host surface. c, d. Section through ascomata. e. Section through peridium. f. Pseudoparaphyses. g–j. Asci. k–p. Ascospores. Scale bars: a = 1 mm, b = 200 μm, c, d = 100 μm, e, f = 20 μm, g–j = 50 μm, k–p = 10 μm. in Two new species of Botryosphaeriaceae (Botryosphaeriales) and new host/ geographical records
FIGURE. Sphaeropsis linhaiensis on dead branch of Cinnamomum camphora (HKAS 10-2383). a, b. Appearance of ascostromata on host surface. c, d. Section through ascomata. e. Section through peridium. f. Pseudoparaphyses. g–j. Asci. k–p. Ascospores. Scale bars: a = 1 mm, b = 200 μm, c, d = 100 μm, e, f = 20 μm, g–j = 50 μm, k–p = 10 μm.
FIGURE 8 in Botryosphaeriaceae associated with Tectona grandis (teak) in Northern Thailand
FIGURE 8. Pseudofusicoccum adansoniae (MFLU 15-0731) a, b. Leaf spot on T. grandis with associated conidiomata. c. Section through conidioma. d–f. Conidia attached to conidiogenous cells. g–o. Conidia. Scale bars: a=1000 μm. b=300 μm.c=30 μm. d=20 μm. e, g–o=10 μm. f=5 μm.
FIGURE 6 in Botryosphaeriaceae associated with Tectona grandis (teak) in Northern Thailand
FIGURE 6. Lasiodiplodia brasiliense (MFLUCC 11-0414) a. Conidia formed on dead branches of Tectona grandis after incubation in a moist chamber for10 days. b. Colony on MEA after 1 week, and inset a conidioma on the agar surface after 1 month. c. Immature conidia attached to conidiogenous cells with paraphyses. d-f. Immature conidia attached to conidiogenous cells. g. Paraphyses. h. Immature conidia. i–k. Mature conidia. Note: c stained with lactophenol cotton blue. c–i, k. Morphology in culture. j morphology on host. Scale bars: a=100 μm. b=300 μm. c–k=10 μm.
FIGURE 7 in Botryosphaeriaceae associated with Tectona grandis (teak) in Northern Thailand
FIGURE 7. Lasiodiplodia pseudotheobromae (MFLU 14-0270) a, b. Conidiomata and conidia on surface of dead twig of Tectona grandis. c. Section through conidioma. d. Conidioma wall. e. Conidia attached to conidiogenous cells with paraphyses. f, g. Immature conidia. h–j. Mature conidia in two different focal planes showing longitudinal striations. k, l. Germinated mature conidia. Note e, g stained with lacto-phenol cotton blue. Scale bars: a=300 μm. b=200 μm. c=100 μm. d, f=20 μm. e, g–l=10 μm.
FIGURE 5 in Botryosphaeriaceae associated with Tectona grandis (teak) in Northern Thailand
FIGURE 5. Dothiorella tectonae (MFLU 14-0272, holotype) a. Conidiomata on dead branch of T. grandis. b. Section through conidioma with conidia becoming dark and aseptate or 1–septate while still attached to conidiogenous cells. c. Conidioma wall. d–e. Conidia attached to conidiogenous cells. f–j. Mature conidia (arrow showing short raised irregular striations on conidia surface). k. Spermatogenous cells and spermatia. l. Germinated mature conidium. Note d, e. stained with lactophenol cotton blue. Scale bars: a=300 μm. b=50 μm. c, e=10 μm. d, f–l=5 μm.
FIGURE 4 in Botryosphaeriaceae associated with Tectona grandis (teak) in Northern Thailand
FIGURE 4. One of 4 most parsimonious trees (CI= 0.912, HI= 0.088, RI= 0.927, RC= 0.845) resulting from combined ITS, TEF1-α and BT analysis for nine taxa in Sphaeropsis species. The tree is rooted to Barriopsis fusca. Maximum parsimony bootstrap values ≥70%, Bayesian posterior probabilities ≥ 0.90 are given at the nodes (MPBS/PP). Type isolates are marked with T. Isolates from this study are in bold.
FIGURE 1 in Botryosphaeriaceae associated with Tectona grandis (teak) in Northern Thailand
FIGURE 1. Phylogenetic trees resulting from individual analyses of each dataset and combined dataset. a. One of>200 most parsimonious trees (CI= 0.622, HI= 0.378, RI= 0.863, RC= 0.537) resulting from ITS analysis for 40 taxa in Dothiorella species, b. Bayesian analysis tree resulting from ITS analysis, c. One of>200 most parsimonious trees (CI= 0.635, HI= 0.365, RI= 0.867, RC= 0.550) resulting from TEF1-α analysis for 39 taxa, d. One of 24 most parsimonious trees (CI= 0.750, HI= 0.250, RI= 0.891, RC= 0.668) resulting from BT analysis for 29 taxa, e. One of 63 most parsimonious trees (CI= 0.642, HI= 0.358, RI= 0.863, RC= 0.554) resulting from combined ITS, TEF1-α and BT analysis for 40 taxa. The tree is rooted to two isolates of Spencermartinsia viticola. Maximum parsimony bootstrap values ≥50%, Bayesian posterior probabilities ≥ 0.90 (MPBS/PP) are given at the nodes. Type isolates are marked with T. Isolates from this study are in blue bold.
FIGURE 2 in Botryosphaeriaceae associated with Tectona grandis (teak) in Northern Thailand
FIGURE 2. One of 48 most parsimonious trees (CI= 0.674, HI= 0.326, RI= 0.881, RC= 0.594) resulting from a combined ITS, TEF1-α and BT analysis for 56 taxa in Lasiodiplodia species. The tree is rooted to isolates of Diplodia mutila and D. seriata. Maximum parsimony bootstrap values ≥50%, Bayesian posterior probabilities ≥ 0.90 (MPBS/PP) are given at the nodes. Type isolates are marked with T. Isolates from this study are in bold.
FIGURE 3 in Botryosphaeriaceae associated with Tectona grandis (teak) in Northern Thailand
FIGURE 3. One of 12 most parsimonious trees (CI= 0.909, HI= 0.091, RI= 0.943, RC= 0.857) resulting from combined ITS and TEF1- α analysis for 20 taxa in Pseudofusicoccum species. The tree is rooted to two isolates of Neofusicoccum parvum. Maximum parsimony bootstrap values ≥50%, Bayesian posterior probabilities ≥ 0.90 (MPBS/PP) are given at the nodes. Type isolates are marked with T. Isolates from this study are in bold.
FIGURE 8 in Resolving Tiarosporella spp. allied to Botryosphaeriaceae and Phacidiaceae
FIGURE 8. Tiarosporella paludosa (CPC 22701). A, B. Conidiomata on PNA. C–E. Conidiogenous cells. F, G. Conidia. Scale bars: A, B = 250 μm, all others = 10 μm.
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