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133 results for “Cactus”
Respirometry data for cactus mice (Peromyscus eremicus) corresponding to Colella et al. 2021
<p>Metabolism is a complex phenotype shaped by natural environmental rhythms, as well as behavioral, morphological, and physiological adaptations. Metabolism has been historically studied under constant environmental conditions, but new methods of continuous metabolic phenotyping now offer a window into organismal responses to dynamic environments, and enable identification of abiotic controls and the timing of physiological responses relative to environmental change. We use indirect calorimetry to characterize metabolic phenotypes of the desert-adapted cactus mouse (<i>Peromyscus eremicus</i>) in response variable environmental conditions that mimic their native environment versus those recorded under constant warm and constant cool conditions, while using a constant photoperiod and full access to resources. We found significant sexual dimorphism, with males being more prone to dehydration than females. Under circadian environmental variation, most metabolic shifts occurred prior to physical environmental change and the timing was disrupted under both constant temperature-humidity treatments. The ratio of CO<sub>2</sub> produced to O<sub>2</sub> consumed (the respiratory quotient) reached greater than 1.0, only during the light phase under diurnally variable conditions, a pattern that strongly suggests that<i> </i>lipogenesis is contributing to the production of energy and endogenous water. Our results are consistent with historical descriptions of circadian torpor in this species (torpid by day, active by night), but reject the hypothesis that torpor is initiated by food restriction or negative water balance.</p>
Effects of powdered cactus pear pruning amendment on the physical and hydraulic properties of two contrasting Mediterranean soils
<p>Full database.</p> <p>A production and consumption paradigm known as "circular economy" (CE) emphasizes sharing, renting, reusing, repairing, refurbishing, and, in particular, recycling materials as much as feasible. Traditional agriculture relied totally on the CE, progressively the search for maximization of yields has produced more and more by-products. Their recovery and reuse are possible with approaches that refer to the CE, for example, with the use of pruning biomasses. The cultivation of the cactus pear annually produces large quantities of pruning residues, which have been shown to be useful for the recovery and reuse of nutrients. This study investigates the hydraulic properties of benchmark soils in which this by-product is incorporated. Here we show that the amendment with powdered cactus pear pruning waste (PCPPW) positively affects soil water retention. However, observable benefits require very high amendment proportions, more than 20% by volume. These quantities make use in the open field unrealistic but offer perspectives in the horticultural and floricultural sectors. These results reveal agreement in direct comparison to what was thought to be the case previously, i.e., a decrease in soil bulk density, an increase in plant available water capacity and an increase in soil swelling. A few per cent application of PCPPW improves the drainable water capacity only in the case of not very clayey soils, where their use becomes useless. The principles of the CE are important, but they must not be pursued a priori. For example, in the use of soil amendments, the behavior in the different soils conditions the suitability of their use.</p>
Respirometry data for cactus mice (Peromyscus eremicus) corresponding to Colella et al. 2021
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A chromosome-level genome assembly of the beavertail cactus, Opuntia basilaris
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Saguaro cactus within-species phylogenomics
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Input data of manuscript "CACTUS: integrating clonal architecture with genomic clustering and transcriptome profiling of single tumor cells"
<p>This is the directory containing input data necessary to reproduce analyses presented in the manuscript:</p> <blockquote> <p><strong>CACTUS: integrating clonal architecture with genomic clustering and transcriptome profiling of single tumor cells</strong><br> Shadi Darvish Shafighi, Szymon M Kiełbasa, Julieta Sepúlveda Yáñez, Ramin Monajemi, Davy Cats, Hailiang Mei, Roberta Menafra, Susan Kloet, Hendrik Veelken, Cornelis A.M. van Bergen, Ewa Szczurek</p> </blockquote>
Large flowers can be short-lived: Insights from a high Andean cactus
<p>Long-lived and large flowers signify high floral maintenance costs. Species of arid/semiarid climates with large flowers are expected to have short flower lifespans and pollination-induced flower longevity in order to curb high floral water and other maintenance costs. We explored the context-dependent large flower/short flower longevity hypothesis in <em>Eriosyce curvispina</em> (Cactaceae), a large-flowered species of the semi-arid central Chilean Andes. We determined breeding system, flower visitation rates, and open-pollination fruit set and quantified floral water content. In a temperature-controlled field manipulative experiment, we measured potential flower longevity and tested for the presence of pollination-induced floral senescence. We measured the timespan of the complete flower cycle defined as from when the flowers began to open to when they were totally closed, and the timespan of fully open flowers defined as from when they were totally open until they began to close. The potential flower lifespan averaged 2.8 days (complete flower cycle) to 2.3 days (fully open flower). The complete flower cycle lasted 21.5 hrs, but flowers were fully open for only 10.1 hrs across days. Flower longevity in days was far shorter than reported for a large sample of species in the area. No evidence was found for pollination-induced flower senescence as a complementary means for reducing floral maintenance costs. <em>Eriosyce curvispina</em> is self-incompatible and abundantly pollinated by two megachilid bees. The level of pollen limitation (<em>L</em>=0.36) was lower than the average reported for self-incompatible angiosperms. Thus, the short flower lifespan in <em>E. curvispina</em> is not an impediment to high fruit set. Flowers contain >5 g of water of which >2 g resides in >40 petaloid tepals. The amount of water is far higher than in another large-flowered, non-cactus species in the area, but only about 15% of that reported in the giant sahuaro cactus, which has larger flowers than <em>E. curvispina</em>.</p>
Variability in mating strategies in a wide spread cactus in the Chihuahuan desert
<p><em>Opuntia</em> is the most diverse and widespread genus in the Cactaceae; several species have broad distribution ranges and have successfully adapted to distinct habitats. One explanation for their success is the diversity of their reproductive systems. <em>Opuntia macrocentra Engelm</em> is one of the most common widespread species in North American deserts. Previous work on a northern population documented that this species has a mixed-mating system and receives a low frequency of pollinators. In a southern population of <em>O. macrocentra</em>, we described its floral visitors, anthesis schedules, and flower morphometry. Through controlled pollination experiments (selfing, outcrossing, supplementary pollen, and natural pollination), we assessed the breeding and mating systems and measured pollen limitation. Eight different bee species visited the flowers. Based on their conduct, <em>Diadasia rinconis</em> and <em>Lasioglossum sp</em>. were the main pollinators. Flowers were homogamous and herkogamous. Floral traits and pollination experiments showed that <em>O. macrocentra</em> is xenogamous and self-incompatible. The fruit set of the natural pollination, outcrossing, and supplementary pollen treatments were not statistically different. The seed set was higher in natural pollination and the pollen limitation index for the seed set was negative. Based on these results, we conclude that <em>O. macrocentra</em> is not pollen limited in the studied population. These results contrast with those of the previously studied population; therefore, we caution about making generalizations about the reproductive system based solely on studies in one population. The diversity of mating systems displayed by <em>O. macrocentra</em> may explain its broad distribution and adaptation to different environmental factors, one of which may be pollinator availability. Our results highlight the need to describe the reproductive ecology of populations instead of species because reproductive systems and ecological factors such as plant-animal interactions may differ across their distribution range.</p>
A hyperspectral scan of a flowering cactus
<p>A hyperspectral scan of a flowering cactus</p>
Data from: Characterization of a male reproductive transcriptome for Peromyscus eremicus (Cactus mouse)
Rodents of the genus Peromyscus have become increasingly utilized models for investigations into adaptive biology. This genus is particularly powerful for research linking genetics with adaptive physiology or behaviors, and recent research has capitalized on the unique opportunities afforded by the ecological diversity of these rodents. Well characterized genomic and transcriptomic data is intrinsic to explorations of the genetic architecture responsible for ecological adaptations. Therefore, this study characterizes the transcriptome of three male reproductive tissues (testes, epididymis and vas deferens) of Peromyscus eremicus (Cactus mouse), a desert specialist. The transcriptome assembly process was optimized in order to produce a high quality and substantially complete annotated transcriptome. This composite transcriptome was generated to characterize the expressed transcripts in the male reproductive tract of P. eremicus, which will serve as a crucial resource for future research investigating our hypothesis that the male Cactus mouse possesses an adaptive reproductive phenotype to mitigate water-loss from ejaculate. This study reports genes under positive selection in the male Cactus mouse reproductive transcriptome relative to transcriptomes from Peromyscus maniculatus (deer mouse) and Mus musculus. Thus, this study expands upon existing genetic research in this species, and we provide a high quality transcriptome to enable further explorations of our proposed hypothesis for male Cactus mouse reproductive adaptations to minimize seminal fluid loss.
PanGenie results generated for Minigraph-Cactus paper
<p><strong>PanGenie results generated for Minigraph-Cactus paper</strong></p> <p>PanGenie (v2.1.0) was used to genotype a subset of 100 trios (300 samples) from the 1000 Genomes based on VCFs generated from the Minigraph-Cactus graphs for GRCh38 and CHM13, as well as the HGSVC VCF. This repository contains the resulting genotypes for SNPs, indels and SVs. See README.txt for details on the files.</p>
Cactus height increases the modularity of a plant-frugivore network in the Caatinga dry forest
<p class="MsoNormal"><span>Cacti fruits are key resources to many frugivorous animals in Neotropical arid and semiarid regions. However, most studies have focused on a particular animal group or cacti species, but few have explored the overall interactions of such species at the community level. Here we monitored frugivory on five cacti species using camera traps that sampled diurnal and nocturnal interactions. We investigated the structure of interactions with bird, mammal, and reptile frugivores in the Brazilian Caatinga dry forest. We hypothesized that the height of cacti limit interactions with different types of frugivores, which would result in highly structured and modular interaction networks. In 2,929 camera-days, we recorded 23 vertebrate species feeding on cacti fruits, including seven new records, all determined to be primary seed dispersers. As predicted, the cacti-frugivore network was modular and non-nested, with the two shortest cacti species grouped in a module dominated by interactions with reptiles and non-flying mammals. The tallest cacti species were dominated by frugivory interactions with birds and had comparatively less interaction diversity than shorter cacti species. Our results support the contention that cacti are keystone species in semiarid ecosystems where they produce small-seeded fleshy fruits year-round.</span></p>
Galindo-Carvajal_Cactus
<p>Structural Analysis of Dried Cactus Leaf</p>
Petit Cactus Study
ClinicalTrials.gov study NCT07380802. IPD Sharing: NO. Countries: 1. Publications: 6.
Data from: Characterization of a male reproductive transcriptome for Peromyscus eremicus (Cactus mouse)
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Living with males leads to female physical injury in the leaf-footed cactus bug
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Cactus height increases the modularity of a plant-frugivore network in the Caatinga dry forest
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Variability in mating strategies in a wide spread cactus in the Chihuahuan desert
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Large flowers can be short-lived: Insights from a high Andean cactus
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Progressive Cactus alignment of 298 drosophilid species
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.